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Characterization of TsFDH-RhM. (A) NAD+-dependent conversion of formate to carbon dioxide by TsFDH-RhM (40 nM), TsFDH-V13pAzF (40 nM), TsFDH-WT (40 nM), and RhM (40 nM); (B) Photo-induced generation of NADH. The reaction mixture consisting of TEOA (5%), eosin Y (20 µM), NAD+ (0.8 mM), and TsFDH-RhM (40 μM) or free RhM (40 μM) was illuminated by white light at 37 °C.
