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. 2016 Jun 8;21(6):745. doi: 10.3390/molecules21060745

Figure 1.

Figure 1

Cytotoxicity testing with IBH, IBM, IBB, and IBW crude fractions. (A) MTT assay for 3T3-L1 preadipocytes (1 × 104 cells/cm2) treated with 0.1 mg/mL of IBH, IBM, IBB, and IBW; (B) Trypan blue assay for 3T3-L1 preadipocytes treated with extract (0.1 mg/mL) for 72 h. Survival and mortality rates of cells are marked in black and gray, respectively; (C) MTT assay of 3T3-L1 differentiated adipocytes (1 × 105 cells/well, 12 wells) treated with 0.1 mg/mL of IBH, IBB, and IBW in 10% FBS medium for 72 h; (D) MTT assay of primary rat hepatocytes (1 × 105 cells/well, 12 wells collagen coating) treated with 0.1 mg/mL of IBH, IBB, and IBW for 24 h. ANOVA statistical analysis, * p < 0.05.