Figure 2.
Apoptotic cell death and regulation of tumor suppressor gene or oncogene of HT-29 cells treated with mature wheat bran (MWB), immature wheat bran (IWB), and steamed immature wheat bran (SIWB) extracts. (A) depicts relative apoptotic cell death results measured with a Cell Death Detection enzyme-linked immunosorbent assay (ELISA) kit; (B) shows Western blotting results; (C) represents observation of cells stained with Annexin V and propidium iodide (PI) by fluorescent microscopy with 40× magnification. ** p < 0.01, *** p < 0.001 vs. C (C, cells without treatment).