Table 4.
Phenolics composition of Licania tomentosa by HPLC-DAD analysis.
| Compounds | LtEE | LtMF | LtEAF | LtCF | LtAF | Calibration Curve | RT | LOQ | LOD | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| mg/g | % | mg/g | % | mg/g | % | mg/g | % | mg/g | % | min | μg/mL | μg/mL | ||
| Gallic acid | 5.17 ± 0.03 a | 0.51 | 6.81 ± 0.01 a | 0.68 | 24.35 ± 0.01 a | 2.43 | 3.67 ± 0.01 a | 0.36 | 6.93 ± 0.02 a | 0.69 | Y = 14,063x + 1187.9 (r = 0.9997) | 12.53 | 0.018 | 0.059 |
| Catechin | 1.43 ± 0.02 b | 0.14 | 1.54 ± 0.01 b | 0.15 | 7.81 ± 0.03 b | 0.78 | 3.51 ± 0.02 a | 0.35 | 1.34 ± 0.01 b | 0.13 | Y = 11,964x + 1387.6 (r = 0.9999) | 15.96 | 0.023 | 0.075 |
| Chlorogenic acid | 22.38 ± 0.01 c | 2.23 | 14.63 ± 0.02 c | 1.46 | 46.59 ± 0.02 c | 4.65 | 14.38 ± 0.02 b | 1.43 | 3.81 ± 0.02 c | 0.38 | Y = 12,850x + 1372.5 (r = 0.9993) | 21.09 | 0.007 | 0.023 |
| Caffeic acid | 14.90 ± 0.01 d | 1.49 | 5.57 ± 0.03 d | 0.55 | 42.63 ± 0.01 d | 4.26 | 3.82 ± 0.02 a | 0.33 | 2.67 ± 0.03 d | 0.26 | Y = 12,748x + 1240.8 (r = 0.9991) | 25.01 | 0.034 | 0.112 |
| Epicatechin | 3.25 ± 0.03 e | 0.32 | 1.30 ± 0.01 b | 0.13 | 9.21 ± 0.03 b | 0.92 | 0.59 ± 0.01 c | 0.05 | 2.72 ± 0.02 d | 0.27 | Y = 12,678x + 1329.7 (r = 0.9990) | 31.87 | 0.015 | 0.049 |
| Ellagic acid | 4.61 ± 0.04 a | 0.46 | 2.14 ± 0.02 b | 0.21 | 31.98 ± 0.01 e | 3.19 | 3.16 ± 0.03 a | 0.31 | 6.83 ± 0.01 a | 0.68 | Y = 12,756x + 1367.1 (r = 0.9996) | 37.26 | 0.011 | 0.034 |
| Rutin | 8.73 ± 0.01 f | 0.87 | 14.09 ± 0.03 c | 1.40 | 20.14 ± 0.01 f | 2.01 | - | - | 7.40 ± 0.02 a | 0.74 | Y = 12,694x + 1357.4 (r = 0.9993) | 43.15 | 0.029 | 0.095 |
| Quercitrin | 3.56 ± 0.01 e | 0.35 | 5.32 ± 0.01 d | 0.53 | 24.17 ± 0.04 a | 2.41 | 0.24 ± 0.01 c | 0.02 | 5.01 ± 0.03 e | 0.50 | Y = 12,571x + 1358.5 (r = 0.9997) | 45.16 | 0.042 | 0.138 |
| Quercetin | 14.27 ± 0.02 d | 1.42 | 9.76 ± 0.01 e | 0.97 | 17.54 ± 0.01 a | 2.75 | - | - | 14.27 ± 0.01 f | 1.42 | Y = 14,274x + 1341.5 (r = 0.9995) | 50.02 | 0.008 | 0.029 |
| Kaempferol | 4.83 ± 0.01 a | 0.48 | 5.41 ± 0.02 d | 0.54 | 2.68 ± 0.01 g | 0.26 | 3.47 ± 0.02 a | 0.34 | 0.89 ± 0.02 g | 0.08 | Y = 13,657x + 1293.8 (r = 0.9999) | 55.19 | 0.021 | 0.069 |
| Kaempferol glycoside | 1.56 ± 0.01 b | 0.15 | 1.70 ± 0.01 b | 0.17 | 8.39 ± 0.01 b | 0.83 | 0.73 ± 0.01 c | 0.07 | 3.15 ± 0.01 d | 0.31 | - | 59.83 | - | - |
Results are expressed as means ± S.E. of three determinations. Concentrations are expressed as mg of each compound per g of sample and in % (g of each compound per 100 g of sample). LtMF: L. tomentosa methanolic fraction; LtEAF: L. tomentosa ethyl acetate fraction; LtCF: L. tomentosa chloroformic fraction and LtAF: L. tomentosa aqueous fraction; LOD: Limit of detection; LOQ: limit of quantification; RT: retention time. Values with different letters differ by Tukey test with p < 0.05.