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Fluorescence synchronous spectra of 5-HOF and complex (2) at Δλ = 15 nm (left) and 60 nm (right). [Tf] = 10 µM, pH = 7.4; [tested compound] = 0; 5; 7.5; 10; 12.5; 15; 17.5; 20 µM. Arrows indicate the changes in fluorescence intensities upon increasing the amounts of the tested compound.
