Figure 2.

HaCaT cells were pre-incubated with TNF-α (10 ng/mL) and IFN-γ (10 ng/mL) to stimulate the cells. After 6 h of stimulation, cells were harvested and total RNA was isolated. Gene expression levels of pro-inflammatory cytokines (TNFα, CCL17, IL-1β, and IL-6) were measured by conventional PCR (A); After 6 h of stimulation, cells were harvested and total protein was isolated. Phosphorylation of ERK1/2 (B) and JNK (D) was inhibited by treatment with L. edodes extract, but that of p38 was not (C). The results provided are representative of three independent experiments. * CSA, cyclosporine A (1 μg/mL); +, treatment; −, no treatment.