The Hedyotis diffusa Willd. (Rubiaceae): A Review on Phytochemistry, Pharmacology, Quality Control and Pharmacokinetics

Rui Chen; Jingyu He; Xueli Tong; Lan Tang; Menghua Liu

doi:10.3390/molecules21060710

. 2016 May 30;21(6):710. doi: 10.3390/molecules21060710

The Hedyotis diffusa Willd. (Rubiaceae): A Review on Phytochemistry, Pharmacology, Quality Control and Pharmacokinetics

Rui Chen ^1,², Jingyu He ³, Xueli Tong ^1,², Lan Tang ^1,², Menghua Liu ^1,^2,^*

Editor: Christopher WK Lam

PMCID: PMC6273454 PMID: 27248992

Abstract

Hedyotis diffusa Willd (H. diffusa) is a well-known Chinese medicine with a variety of activities, especially its anti-cancer effect in the clinic. Up to now, 171 compounds have been reported from H. diffusa, including 32 iridoids, 26 flavonoids, 24 anthraquinones, 26 phenolics and their derivatives, 50 volatile oils and 13 miscellaneous compounds. In vitro and in vivo studies show these phytochemicals and plant extracts to exhibit a range of pharmacological activities of anti-cancer, antioxidant, anti-inflammatory, anti-fibroblast, immunomodulatory and neuroprotective effects. Although a series of methods have been established for the quality control of H. diffusa, a feasible and reliable approach is still needed in consideration of its botanical origin, collecting time and bioactive effects. Meanwhile, more pharmacokinetics researches are needed to illustrate the characteristics of H. diffusa in vivo. The present review aims to provide up-to-date and comprehensive information on the phytochemistry, pharmacology, quality control and pharmacokinetic characteristics of H. diffusa for its clinical use and further development.

Keywords: H. diffusa, phytochemistry, pharmacology, quality control, pharmacokinetics

1. Introduction

Hedyotis diffusa Willd (H. diffusa, Family Rubiaceae), known as Oldenlandia diffusa (Willd) Roxb, is a well-known Chinese medicine used for the treatment of inflammation-linked diseases, such as hepatitis, appendicitis and urethritis, for thousands of years in China [1]. In our previous studies, the water extract of H. diffusa has been proved to have an obvious protective effect in lipopolysaccharide-induced renal inflammation in mice. Recently, H. diffusa has gained increasing attention for its properties of anti-proliferative activity in cancer cells and anti-tumor activity in tumor-bearing animals [2,3,4,5]. It has been proved as the most commonly prescribed single Chinese herb used for colon cancer and breast cancer patients [6,7], according to the statistics from the National Health Insurance Research Database of Taiwan.

H. diffusa is an annual herb, widely distributed in the orient and tropical Asia, such as China, Japan and Indonesia [1,8]. Generally, the plant grows in humid fields and ridges of farmlands, ascending to procumbent, to 50 cm tall; the stem is slightly flattened to terete, glabrescent to glabrous and the papilla was observed in the transverse section of the stem; the leaves are opposite, sessile or subsessile and blade drying membranous, linear, narrowly elliptic, 1–4 × 0.1–0.4 cm; the flowers with pedicels are pairs in axillary racemes and the corolla is white [1,9]. Together with these phenotypic characteristics of H. diffusa, methods of thin-layer chromatography (TLC) [10], gas chromatography-mass spectrometer (GC-MS) [11], high performance liquid chromatography (HPLC) [9] and DNA sequencing [8,12] have been developed to differentiate H. diffusa from related species (e.g., Hedyotis corymbosa (L.) Lam) to give the right prescription for illnesses.

Although there are numbers of published scientific literature on the chemical constituents, pharmacological activities and quantitative analysis of H. diffusa, a systematic and updated review is unavailable. Therefore, the aim of this review is to extensively summarize the phytochemistry, pharmacology, quality control and pharmacokinetic characteristics of H. diffusa, as well as being an evidence for clinical uses and further researches of this herb.

2. Phytochemistry

With the advancement of analysis technologies like mass spectrometer (MS), liquid chromatograph–mass spectrometer (LC-MS), nuclear magnetic resonance–mass spectrometer (NMR-MS) etc., many studies on H. diffusa revealed numbers of important phytochemicals, including iridoids, triterpenes, flavonoids, anthraquinones, phenolic acids and their derivatives, sterols, alkaloids, volatile oils, polysaccharides, cyclotides, coumarins and alkaloids. The detailed information for these compounds is summarized in Table 1.

Table 1.

Compounds of the H. diffusa.

NO.	Compound Name	Molecular Formula	Reference
Iridoids
1	Asperuloside	C₁₈H₂₂O₁₁	[13,14]
2	Deacetyl asperuloside	C₁₆H₂₀O₁₀	[15]
3	Asperuloside acid	C₁₈H₂₄O₁₂	[16]
4	Deacetyl asperulosidic acid	C₁₆H₂₂O₁₁	[15]
5	Deacetyl asperulosidic acid methyl ester	C₁₇H₂₄O₁₁	[15,17]
6	Geniposidic acid	C₁₆H₂₂O₁₀	[18]
7	10-O-Acetyl geniposidic acid	C₁₈H₂₄O₁₁	[15]
8	10-Dehydro geniposide	C₁₇H₂₂O₁₀	[17]
9	10-Dehydro geniposidic acid	C₁₆H₂₀O₁₀	[19]
10	Diffusoside A	C₁₉H₂₈O₁₁	[20]
11	Diffusoside B	C₁₉H₂₈O₁₁	[20]
12	Lupenylacetate	C₃₂H₅₂O₂	[21]
13	Alpigenoside	C₁₈H₂₈O₁₂	[15]
14	Oldenlandoside III	C₃₄H₄₄O₂₀	[22]
15	5-O-Feruloyl scandoside methyl ester	C₂₇H₃₂O₁₄	[23]
16	Hehycoryside C	C₂₃H₂₆O₁₁	[22]
17	6-α-Hydro scandoside	C₁₆H₂₂O₁₁	[24]
18	6-β-Hydro scandoside	C₁₆H₂₂O₁₁	[24]
19	6-Dehydro scandoside	C₁₆H₂₂O₁₀	[19]
20	6-α-Hydro scandoside methyl ester	C₁₇H₂₄O₁₁	[24]
21	6-β-Hydro scandoside methyl ester	C₁₇H₂₄O₁₁	[24]
22	6-α-Hydro-10-acetyl asperuloside acid	C₁₈H₂₄O₁₂	[24]
23	6-β-Hydro-10-acetyl asperuloside acid	C₁₈H₂₄O₁₂	[24]
24	6-O-Methoxyl cinnamoyl scandoside	C₂₇H₃₂O₁₃	[23]
25	6-O-p-Hydro cinnamoyl scandoside	C₂₆H₃₀O₁₃	[23]
26	(E)-6-O-p-Coumaroyl-10-O-formoxyl scandoside methyl ester	C₂₇H₃₂O₁₃	[14]
27	(E)-6-O-p-Coumaroyl scandoside methyl ester	C₂₆H₃₀O₁₃	[14,15,25]
28	(Z)-6-O-p-Coumaroyl scandoside methyl ester	C₂₆H₃₀O₁₃	[18]
29	(E)-6-O-p-Methoxy cinnamoyl scandoside methyl ester	C₂₇H₃₂O₁₃	[15,25,26]
30	(Z)-6-O-p-Methoxy cinnamoyl scandoside methyl ester	C₂₇H₃₂O₁₃	[26]
31	(E)-6-O-Feruloyl scandoside methyl ester	C₂₇H₃₂O₁₄	[15,25,26]
32	(Z)-6-O-Feruloyl scandoside methyl ester	C₂₇H₃₂O₁₄	[27]
Triterpenes
33	Arborinone	C₃₀H₄₈O	[28]
34	Isoarborinol	C₃₀H₅₀O	[28]
35	Oleanolic acid	C₃₀H₄₈O₃	[19]
36	Ursolic acid	C₃₀H₄₈O₃	[19]
Flavonoids
37	Amentoflavone	C₃₀H₁₈O₁₀	[26,29]
38	Chrysin-6-C-glucosyl-8-C-arabinosyl	C₂₆H₂₈O₁₃	[22]
39	Chrysin-6-C-arabinosyl-8-C-glucosyl	C₂₆H₂₈O₁₃	[22]
40	Oroxylin-A-O-glucuronic acid	C₂₂H₂₀O₁₁	[22]
41	Wogonin-O-glucuronic acid	C₂₂H₂₀O₁₁	[22]
42	5,7-Dihydroxy-3-methoxy flavonol	C₁₆H₁₂O₅	[13]
43	5,7,4′-Trihydroxy flavonol	C₁₅H₁₀O₆	[13]
44	5-Hydroxy-6,7,3′,4′-tetramethoxy flavone	C₁₉H₁₈O₇	[21]
45	Quercetin	C₁₅H₁₀O₇	[17,19,30]
46	Rutin	C₂₇H₃₀O₁₆	[15,25,31]
47	Quercetin-3-O-β-d-glucopyranside	C₂₁H₂₀O₁₂	[25,32,33]
48	Quercetin-3-O-β-d-galactopyranoside	C₂₁H₂₀O₁₂	[32]
49	Quercetin-3-O-(2-O-glucopyranosyl)-β-d-glucopyranside	C₂₇H₃₀O₁₇	[15,25,32,33]
50	Quercetin-3-O-(2-O-glucopyranosyl)-β-d-galactopyranoside	C₂₇H₃₀O₁₇	[11,34]
51	Quercetin-3-O-sambubioside	C₂₆H₂₈O₁₆	[15,25]
52	Quercetin-3-O-[2-O-(6-O-E-ferloyl)-β-d-glucopyranosyl]-β-d-galactopyranoside	C₃₇H₃₈O₂₀	[11,34]
53	Quercetin-3-O-[2-O-(6-O-E-feruloyl)-β-d-glucopyranosyl]-β-d-glucopyanoside	C₃₇H₃₈O₂₀	[11,15,25]
54	Quercetin-3-O-[2-O-(6-O-E-sinapoyl)-β-d-glucopyranosyl]-β-d-glucopyanoside	C₃₈H₄₀O₂₁	[15]
55	Quercetin-3-O-[2-O-(6-O-E-sinapoyl)-β-d-glucopyranosyl]-β-d-galactopyranoside	C₃₈H₄₀O₂₁	[25]
56	Kaempferol	C₁₅H₁₀O₆	[17,35]
57	Kaempferol-3-O-β-d-glucopyranside	C₂₁H₂₀O₁₁	[32]
58	Kaempferol-3-O-β-d-galactopyranoside	C₂₁H₂₀O₁₁	[32]
59	Kaempferol-3-O-(2-O-β-d-glucopyranosyl)-β-d-galactopyranoside	C₂₇H₃₀O₁₆	[11,25,34]
60	Kaempferol-3-O-(6-O-α-l-rhamnosyl)-β-d-glucopyranside	C₂₇H₃₀O₁₆	[32]
61	Kaempferol-3-O-[2-O-(E-6-O-feruloyl)-β-d-glucopyranosyl]-β-d-glucopyranosyl	C₃₇H₃₈O₁₉	[11,25,33]
62	Kaempferol-3-O-[2-O-(6-O-E-feruloyl)-β-d-glucopyranosyl]-β-d-galactopyranoside	C₃₇H₃₈O₁₉	[21,34]
Athraquinones
63	2-Methyl-3-methoxy anthraquinone	C₁₆H₁₂O₃	[19]
64	2-Hydroxy-1,3-dimethoxy anthraquinone	C₁₆H₁₂O₅	[29]
65	2-Hydroxy-3-methyl-1-methoxy anthraquinone	C₁₆H₁₂O₄	[36]
66	2-Hydroxy-3-methyl-4-methoxy anthraquinone	C₁₆H₁₂O₄	[37]
67	2-Hydroxy-7-methyl-3-methoxy anthraquinone	C₁₆H₁₂O₄	[36]
68	2-Hydroxy-1-methoxy-3-methyl anthraquinone	C₁₆H₁₄O₄	[31]
69	2-Hydroxy-3-methyl anthraquinone	C₁₅H₁₀O₃	[17,27]
70	2-Hydroxy-1-methoxy anthraquinone	C₁₅H₁₀O₄	[27,29]
71	2-Hydroxy-4-methoxy anthraquinone	C₁₅H₁₀O₄	[38]
72	2-Hydroxy-3-methoxy-7-methyl anthraquinone	C₁₆H₁₂O₄	[36]
73	2-Hydroxy-6-methyl anthraquinone	C₁₅H₁₀O₃	[18]
74	2-Hydroxy-3-methoxy-6-methyl anthraquinone	C₁₆H₁₂O₄	[18]
75	2,7-Dihydroxy-3-methyl anthraquinone	C₁₅H₁₀O₄	[39]
76	3-Hydroxy-2-methyl anthraquinone	C₁₅H₁₀O₃	[19]
77	3-Hydroxy-2-methyl-4-methoxy anthraquinone	C₁₆H₁₂O₄	[40]
78	2,3-Dimethoxy-6-methyl anthraquinone	C₁₇H₁₄O₄	[18]
79	1,3-Dihydroxy-2-methyl anthraquinone	C₁₅H₁₀O₄	[41]
80	1,7- Dihydroxy-6-methoxy-2-methyl anthraquinone	C₁₆H₁₂O₅	[41]
81	3-Hydroxy-2-methyl-4-methoxy anthraquinone	C₁₆H₁₀O₄	[18]
82	2,6-Dihydroxy-3-methyl-4-methoxy anthraquinone	C₁₆H₁₂O₅	[42]
83	2,6-Dihydroxy-1-methoxy-3-methyl anthraquinone	C₁₆H₁₂O₅	[31]
84	1-Hydroxy-4-methoxy anthraquinone	C₁₅H₁₀O₄	[43]
85	2-Hydroxymethy-1-hydroxy anthraquinone	C₁₅H₁₀O₄	[5]
86	2-Hydroxymethyl anthraquinone	C₁₅H₁₀O₃	[5]
Phenolic acids and their derivatives
87	3,4-Dihydroxy benzoic acid	C₇H₆O₄	[21]
88	4-Hydroxy-3-methoxy benzoic acid	C₈H₈O₄	[30]
89	trans-Hydroxybenzoic acid	C₇H₆O₃	[30]
90	4-Hydroxy-3,5-dimethoxy benzoic acid	C₉H₁₀O₅	[30]
91	p-Coumaric acid	C₉H₈O₃	[19,29]
92	p-Coumaric acid-O-glucopyranside	C₁₅H₁₈O₈	[22]
93	Caffeic acid	C₉H₈O₄	[21]
94	Caffeoyl hexoside	C₁₅H₁₈O₉	[22]
95	Ferulic acid	C₁₀H₁₀O₄	[41]
96	Ferulic acid hexoside	C₁₆H₂₀O₉	[22]
97	p-Methoxy cinnamic acid	C₁₀H₁₀O₃	[44]
98	4,4′-Dihydroxy-α-truxillic acid	C₁₈H₁₆O₆	[44]
99	4,4′-Dimethoxyl-α-truxillic acid	C₁₉H₁₈O₆	[45]
100	Octadecyl (E)-p-coumarate	C₂₇H₄₄O₃	[46]
101	3-Caffeoyl quinic acid	C₁₆H₁₈O₉	[22]
102	4-Caffeoyl quinic acid	C₁₆H₁₈O₉	[22]
103	5-Caffeoyl quinic acid	C₁₆H₁₈O₉	[22]
104	3-р-Coumaroyl quinic acid	C₁₆H₁₈O₈	[22]
105	4-р-Coumaroyl quinic acid	C₁₆H₁₈O₈	[22]
106	5-р-Coumaroyl quinic acid	C₁₆H₁₈O₈	[22]
107	3-Feruloyl quinic acid	C₁₇H₂₀O₉	[22]
108	4-Feruloyl quinic acid	C₁₇H₂₀O₉	[22]
109	5-Feruloyl quinic acid	C₁₇H₂₀O₉	[22]
Sterols
110	Daucosterol	C₃₅H₆₀O₆	[19]
111	β-Sitosterol	C₂₉H₅₀O	[19]
112	Stigmasterol	C₂₉H₄₈O	[17,19]
113	Stigmasterol-5,2-diene-3β, 7α-glycol	C₂₉H₄₈O₂	[47]
Volatile oils
114	6,10,14-Trimethyl-2-pentadecanone	C₁₈H₃₆O	[48]
115	Phytol	C₂₀H₄₀O	[48]
116	α-Cedrol	C₁₅H₂₆O	[48]
117	Tetradecanoic acid	C₁₄H₂₈O₂	[48]
118	Hexadecanoic acid, methyl ester	C₁₇H₃₄O₂	[48]
119	Hexadecanoic acid,	C₁₆H₃₂O₂	[48]
121	1,2-Benzenediearboxylic acid isobutyl ester	C₁₆H₂₂O₄	[48]
122	1,2-Benzenediearboxylic acid, bis(2-methylpropyl)ester	C₁₆H₂₂O₄	[48]
123	9,12,15-Octadecatrienoic acid, methyl ester	C₁₉H₃₂O₂	[48]
124	9-Octadecenoic acid	C₁₈H₃₄O₂	[48]
125	9,12-Octadecenoic acid	C₁₈H₃₂O₂	[48]
126	Ethyl linoleate	C₂₀H₃₆O₂	[48]
127	Triethyl phosphate	C₆H₁₅O₄P	[48]
128	4-Vinyl phenol	C₈H₈O	[48]
129	2-Methoxy-4-vinylphenol	C₉H₁₀O₂	[48]
130	n-Pentadecanoic acid	C₁₅H₃₀O₂	[48]
131	4,8,12,16-Tetramethyl heptadecan-4-olide	C₂₁H₄₀O₂	[48]
132	2,6,10,14,18,22-Tetracosahexaene	C₃₀H₅₀	[48]
133	α-Terpineol	C₁₀H₁₈O	[11]
134	Geranyl acetate	C₁₂H₂₀O₂	[11]
135	β-Ionone	C₁₃H₂₀O	[11]
136	Lauric acid	C₁₂H₂₄O₂	[11]
137	Myristic acid	C₁₄H₂₈O₂	[11]
138	Palmitic acid	C₁₆H₃₂O₂	[11]
139	Linoleic acid	C₁₈H₃₂O₂	[11]
140	β-Linalool	C₁₀H₁₈O	[11]
141	Isoborneol	C₁₀H₁₈O	[49]
142	3-(2-Propenyl)-cyclohexene	C₉H₁₄	[49]
143	2-Pentyl-furam	C₉H₁₄O	[49]
144	Cis-2-(2-pentenyl)-furan	C₉H₁₂O	[49]
145	Limonene	C₁₀H₁₈	[49]
146	3,7-Dimethyl-1,6-octadiem-3-ol	C₁₀H₁₈O	[49]
147	trans-5-Methyl-2-(1-methylethyl)-cyclohexanope	C₁₀H₁₈O	[49]
148	(1S-endo)-1,7,7-Trimethyl-bicyclo[2,2,1]heptan-2-ol	C₁₀H₁₈O	[49]
149	p-Menth-1-en-8-ol	C₁₀H₁₈O	[49]
150	Pulegone	C₁₀H₁₆O	[49]
151	4-(2,6,6-Trimethyl-1-cyclohexen-1-yl)-3-buten-2-one	C₁₃H₂₀O	[49]
152	Hexadecanal	C₁₆H₃₂O	[49]
153	2,6,10,14-Tetramethyl-hexadecane	C₂₀H₄₂	[49]
154	(Z,Z)-9,12-octadecadienoic acid	C₁₈H₃₂O₂	[49]
155	(Z)-9,17-octadecadienal	C₁₈H₃₂O	[49]
156	Cis,cis,cis-7,10,13-hexadecatrienal	C₁₆H₂₆O	[49]
157	Oleic acid	C₁₈H₃₄O₂	[49]
158	Hexaldehyde	C₆H₁₂O	[49]
159	Borneol	C₁₀H₁₈O	[49]
160	Docosane	C₂₂H₄₆	[49]
161	Tetracosane	C₂₄H₅₀	[49]
162	Hexacosane	C₂₆H₅₄	[49]
163	Heptacosane	C₂₇H₅₆	[49]
Polysaccharides
164	ODP-1		[50]
Cyclotides
165	CD1		[51]
166	CD2		[51]
167	CD3		[51]
Coumarins
168	7-Hydroxy-6-methoxy-Coumarin	C₁₀H₈O₄	[17]
169	Esculetin	C₉H₆O₄	[46]
Alkaloids
170	10(S)-hydroxy pheophytin a	C₅₅H₇₄N₄O₆	[52]
171	Aurantiamide acetate	C₂₇H₂₈N₂O₄	[46]

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2.1. Iridoids and Triterpenes

Iridoids are one of the most important components in H. diffusa with various bioactivities, such as anti-oxidant, neuroprotective and anti-inflammatory effects [33,53]. Accompanied with the analysis of the NMR spectra of the pure compounds, the methods of tandem mass spectrometry (MSⁿ) and time-of-flight mass spectrometry (TOF/MS) have become more popular for the identification of these compounds [11,14,15,25,52]. To date, thirty-two iridoids and their iridoid glucosides (1–32) have been isolated and identified from H. diffusa (Figure 1).

Chemical structures of iridoids and triterpenes in *H. diffusa*.

Four triterpenes, named arborinone (33), isoarborinol (34), oleanolic acid (35) and ursolic acid (36), were isolated from H. diffusa and their structures were established by 1D-, 2D-NMR spectroscopic analysis and high-resolution electrospray ionization mass spectroscopy (HRESIMS) [53].

2.2. Flavonoids

Flavonoids are a major group presented in H. Diffusa, and most of them are derivatives of the flavonol aglycones of kaempferol and quercentin. Recently, other aglycones, such as chrysin, oroxylin and wogonin, have been characterized by ultra-performance liquid chromatography–diode array detector/quadrupole time-of-flight mass spectrometry (UPLC-DAD/Q-TOF-MS). To date, twenty-six flavonoids (37–62) with various substitutions have been identified and their chemical structures are prescribed in Figure 2.

Chemical structures of flavonoids in *H. diffusa*.

2.3. Anthraquinones

Anthraquinones are also a major group of bioactive components in H. diffusa. Up to now, twenty-four anthraquinones with various substitutions (63–86) have been obtained and identified from this herb. These compounds have a typical characteristic of the 9, 10-anthraquinone skeleton with the presence of hydroxy, methyl and/or methoxy groups, for example, 2-hydroxy-3-methoxy-6-methyl anthraquinone (77). Their chemical structures are shown in Figure 3.

Chemical structures of anthraquinones in *H. diffusa*.

2.4. Phenolic Acids and Their Derivatives

Phenolic acids are very common and important secondary metabolites in nature. To date, twenty-three phenolic acids (87–109) have been identified from the herb of H. Diffusa, including four benzoic acid derivatives (87–90), coumaric acid (91) and its derivative (92), caffeic acid (93) and its derivative (94), ferulic acid (95) and its derivative (96), p-methoxyl cinnamic acid (97), two truxillic acid derivatives (98–99), octadecyl (E)-p-coumarate (100) and nine quinic acid derivatives (101–109). Their chemical structures are prescribed in Figure 4.

Chemical structures of phenolic acids and their derivatives in *H. diffusa*.

2.5. Polysaccharides

The polysaccharides in H. diffusa have been researched for their immuno-enhancing activity. They are mainly composed of glucose, galactose and mannose, with the content of 15.10% determined by the spectrophotometry method at 490 nm [54]. Up to now, only one homogeneous polysaccharide, ODP-1, has been separated from H. diffusa, with the relative molecular weight of 20.88 kDa. It consists of mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose, with the molar ratio of 0.005:0.033:0.575:1.000:0.144:0.143 [50].

2.6. Essential Oils

The reports of essential oils in this plant were mainly on isolating many fatty acids, fatty acid esters, etc. [11]. Yang et al. [49] identified 29 compounds representing 81.45% of the total oil content by GC/MS combined with the Kovats Retention index. n-Hexadecanoic acid (119) (31.22%), oleic acid (157) (6.74%), tetracosane (161) (4.94%) and 9,12-octadacadienoic acid (125) (4.87%) were found to be the main constituents. Liu et al. [48] compared the constituents and their content in H. diffusa collected from the provinces of Guangdong, Jiangxi and Guangxi in China and also revealed that the oil of H. diffusa was mainly composed of fatty acids with an oil extraction rate from 0.25% to 0.30%.

2.7. Cyclotides

Three novel cyclotides from H. diffusa, named CD1 (165), CD2 (166) and CD3 (167), with an anti-cancer effect on prostate cancer cells, were reported by Hu et al. [51]. The primary sequences were GAFLKCGESCVYLPCLTTVVGCSCQNSVCYRD, GAVPCGETCVYLPCITPDIGCSCQNKVCYRD and G-TSCGETCVLLPCLSSVLGCTCQNKRCYKD for DC1, DC2 and DC3, respectively.

2.8. Miscellaneous

Only four sterols of daucosterol (110), β-sitosterol (111), stigmasterol (112) and stigmasterol-5,2-diene-3β, 7α-glycol (113), two coumarins of 7-hydroxy-6-methoxy-coumarin (168) and esculetin (169) and two alkaloids of 10-hydroxypheophytin a (170) and aurantiamide acetate (171) have been purified and characterized from H. diffusa and their structures are shown in Figure 5.

Chemical structures of miscellaneous components in H. *diffusa*.

3. Pharmacology

H. diffusa has long been used therapeutically in China, due to its broad spectrum of biological and pharmacological activities. Now we have enlisted an overview of the modern pharmacological studies in the following sections (Table 2).

Table 2.

Pharmacological effects of H. diffusa.

Activities	Model	Formulation/Dosage/Extract		Reference
Anti-tumor activity
Colorectal cancer	HT-29 cells	Ethanol extract	The extract suppressed HT-29 cell growth and induced apoptosis via inactivation of the IL-6/STAT3-signaling pathway.	[2]
	HT-29 cells	Ethanol extract	The extract reduced HT-29 cell viability and survival. It could suppress cancer cell proliferation by blocking the cell cycle, preventing G₁ to S progression, and reducing mRNA expression of pro-proliferative PCNA, Cyclin D1 and CDK4, but increasing that of anti-proliferative p21.	[55]
	HT-29 cells	Ethanol extract	The extract induced the HT-29 cell morphological changes and reduced cell viability. In addition, the extract treatment resulted in DNA fragmentation, loss of plasma membrane asymmetry, collapse of mitochondrial membrane potential, activation of caspase-9 and caspase-3 and increase of the ratio of pro-apoptotic Bax to anti-apoptotic Bcl-2.	[56]
	HT-29 cells	Ethanol extract	The extract treatment downregulated the mRNA and protein expression levels of VEGF-A in HT-29 human colon carcinoma cells.	[57]
	HT-29 cells	Ethanol extract	The extract inhibits colorectal cancer growth in vivo via inhibition of SHH-mediated tumor angiogenesis.	[58]
	CRC mouse xenograft model	Ethanol extract	The extract inhibited the expression of the gene VEGF-A and VEGFR2, thus, suppressed the activation of Sonic hedgehog (SHH)-signaling in CRC xenograft tumors; it inhibits colorectal cancer growth.	[58]
	CRC mouse xenograft model	Ethanol extract	The extract suppressed the STAT3 pathway by suppressing STAT3 phosphorylation in tumor tissues, altering the expression pattern of target genes of Cyclin D1, CDK4 and Bcl-2, as well as upregulating p21 and Bax.	[59]
	CT-26 cells	Ethanol extract	The extract can inhibit the proliferation of CT-26 colon cancer cells from BALB/c mice in a time- and dose- dependent manner.	[60]
	HCT-8/5-FU cells	Ethanol extracts	The extract treatment significantly reduced the cell viability of HCT-8/5-FU cells by downregulating the expression of P-gp and ABCG2.	[61]
	Caco-2 cells	Aqueous extracts	The decoction of H. diffusa and its fraction 9 contained sufficient ursolic acid and oleanolic acid to possibly induce apoptosis of Caco-2 cells.	[62]
	Caco-2 cells	Nine pure compounds isolated from H. diffusa	2-Hydroxymethy-1-hydroxy anthraquinone (IC₅₀ 45 mM) and ursolic acid (IC₅₀ 71 mM) exhibited the highest inhibition of Caco-2 cell proliferation.	[5]
Leukemia	CEM cells	Aqueous extract	The extract inhibited Leukemia CEM cells growth in time- and concentration-dependent manners. And the inhibition mechanism has greater correlation with the upregulation of P53 expression.	[63]
	BALB/c mice	Aqueous extract	The extract had anti-leukemia effects on WEHI-3 cell-induced leukemia in vivo.	[64]
	HL-60 cells	H. diffusa injection	The extract could induce HL-60 cells differentiation, and suppress the expression of the anti-apoptosis-related gene to inhibit the growth of HL-60 cells.	[65]
	HL-60 cells, WEHI-3 cells	Ethanol extract	The extract inhibited the cell proliferation of HL-60 cells. It triggered an arrest of HL-60 cells at the G₀/G₁ phase and sub-G₁ population, provoked DNA condensation and DNA damage, but the activities of caspase-3, caspase-8, and caspase-9 were elevated in H. diffusa-treated HL-60 cells.	[66]
	U937 cells	2-Hydroxy-3-methyl anthraquinone	2-Hydroxy-3-methyl anthraquinone enhanced apoptosis of U937 cells through the activation of p-p38MAPK and downregulation of p-ERK1/2.	[67]
	THP-1 Cells	2-Hydroxy-3-methyl anthraquinone	2-Hydroxy-3-methyl anthraquinone induced THP-1 cell apoptosis, which was associated with a more prominent induction expression of Fas/FasL, DR4 and TRAIL. Moreover, 2-Hydroxy-3-methylanthraquinone treatment resulted in activation of caspase-8.	[68]
Liver cancer	H22 mice	Aqueous extract	The extract had an inhibitory effect on the metastasis of hepatocarcinoma in blood.	[69]
	HepG2 cells	Aqueous extract	The extract remarkably inhibited HepG2 cell proliferation via arrest of HepG2 cells at the G₀/G₁ phase and induction of S phase delay. In addition, the extract potentiated the anticancer effect of low-dose 5-FU in the absence of overt toxicity by downregulating the mRNA and protein levels of CDK2, cyclin E and E2F1.	[70]
	MHCC97-H cells	Total flavones extract	The extract treatment reduced the level of E-cadherin protein and increased the expression of vimentin protein in TGF-β1-induced MHCC97-H.	[71]
	HepG2 cells	1,3-Dihydroxy-2-Methylanthraquinone Ethyl acetate extract	Both 1,3-Dihydroxy-2-Methylanthraquinone and ethyl acetate extract exhibited an inhibitory effect on HepG2 cells, resulting in in upregulation of Bax, p53, Fas, FasL, p21 and cytoplasmic cytochrome C levels and caspase-3, -8, -9 proteases activities, while downregulating Bcl-2, mitochondrial cytochrome C, cyclin E and CDK 2 in a dose-dependent manner.	[72]
	HepG2 cells	Nine pure compounds isolated from H. diffusa	Ursolic acid exhibited a strong inhibition of cell survival with C₅₀ 37 mM.	[5]
	HepG2 cells	2-Hydroxy-3-methyl anthraquinone 1-Methoxy-2-hydroxy anthraquinone	Both compounds showed inhibitory activity against protein tyrosine kinases v-src and pp60src and arrested the growth of HepG2 cancer cells.	[38]
Lung cancer	A549 cells, H1355 cells, LLC cells	Ethanol extract	The extract suppressed the cell proliferation of A549 and H1355 cells as well as reduced cell viability in a concentration-dependent manner.	[66]
	SPC-1-A cells	2-Hydroxy-3-methyl anthraquinone 1-Methoxy-2-hydroxy anthraquinone	Both compounds showed inhibitory activity against protein tyrosine kinases v-src and pp60src and arrested the growth of SPC-1-A.	[38]
Breast cancer	MCF-7 cells	Compounds of anthraquinones, iridoid glucosides, stigmasterols and alkaloids/flavonoids	Alkaloids/flavonoids possessed antitumor activity against the human breast cancer cell line MCF7	[73]
	MCF-7 cells	Methyl anthraquinone	Methyl anthraquinone-induced MCF-7 cells apoptosis via Ca²⁺/calpain/caspase-4 pathway.	[74]
	Bcap37 cells	2-Hydroxy-3-methyl anthraquinone, 1-Methoxy-2-hydroxy anthraquinone	Both compounds showed inhibitory activity against protein tyrosine kinases v-src and pp60src and arrested the growth of Bcap37 cells.	[38]
Cervical tumor	Nude mouse model	Aqueous extract	The extract had an inhibitory effect on cervical cancer cells with the expression of Ki-67 protein significantly decreased, and the mean survival time of the mice was significantly extended.	[3]
	HeLa cells	Nine pure compounds isolated from H. diffusa	2-Hydroxymethy-1-hydroxy anthraquinone exhibited the strongest inhibitory effect on cell viability.	[5]
Prostate Cancer	DU145 cells, PC-3 cells LNCaP cells	Nine pure compounds isolated from H. diffusa	2-Methyl-3-methoxy anthraquinone, 2-hydroxy-3-methyl anthraquinone and ursolic acid exhibited inhibitory effects on prostate cancer cell survival.	[5]
	PC3 cells LNCaP cells	6-O-(E)-p-Coumaroyl scandoside methyl ester 10(S)-Hydroxy pheophytin	Two compounds showed a moderate anti-proliferation effect on PC3 human androgen-independent prostate cancer cells, while 10(S)-hydroxy pheophytin also showed a strong anti-proliferation effect on LNCaP human androgen-sensitive prostate cancer cells.	[52]
Multiple myeloma	RPMI 8226 cells	Nine pure compounds isolated from H. diffusa	2-Hydroxymethy-1-hydroxy anthraquinone exhibited the strongest inhibition of RPMI 8226cells growth.	[5]
	RPMI 8226 cells	Polysaccharides extracts	Polysaccharides extracts suppressed the growth of RPMI 8226 cells in a dose- and time-dependent manner.	[75]
	RPMI 8226 cells	H. diffusa injection	H. diffusa injection could inhibit the proliferation of RPMI 8226 cells.	[76]
Others	B16F10 cells	Ethanol extract	The extract suppressed the cell proliferation of B16F10 cells as well as reducing cell viability in a concentration-dependent manner.	[66]
	S180 cells	Decoction, lipophilic extract, crude polysaccharide	Lipophilic extract and crude polysaccharide showed anti-tumor activities and a protective effect on chemotherapeutic damage. However, the aqueous extract had no marked anti-tumor effect on S-180 cells.	[77]
	MG-63cells	H. diffusa injection	H. diffusa injection could inhibit the proliferation of MG-63 cells, and Bax gene expression was significantly increased.	[78]
	MG-63 cells	H. diffusa injection	H. diffusa injection could induce the apoptosis of MG-63 cells by increasing Bax gene expression in a concentration-dependent manner.	[79]
	MG-63 cells	Aqueous extract	H. diffusa, combined with cisplatin, had a stronger inhibitory effect than the single agents in MG-63 cells with IC₅₀164.6 and 5.0 μL/mL, respectively. As a result, H. diffusa could alter anti-apoptotic (Bax and Bad) and pro-apoptotic protein (Bcl-xl and Bcl-2) expression, and it elevated the levels of caspase-3 and caspase-8.	[80]
	U87 cells	Aqueous extract	The extract suppressed U87 cells growth in a dose- and time-dependent manner.	[4]
Angiogenesis	1.Breast tumor-bearing BALB/c mice 2. Zebrafish embryo model 3. Human endothelial cells 4. C57BL/6 mice	4-Vinyl phenol	4-Vinyl phenol was demonstrated with anti-angiogenic activity in vitro and in vivo.	[81]
Immunomodulatory effect
	Normal BALB/c mice	Ethanol Extract	The extract has promoted immune responses in normal BALB/c mice.	[82]
	Immunosuppression mice induced by cyclophosphamide	Polysaccharides extracts	The extract could improve the clearance index, phagocytic index, and the index of the thymus and spleen of immunosuppression mice.	[50]
	Inmmunosuppressed mice induced by cyclophosphamide	Total flavonoids extract	The extract enhanced specific and non-specific immunity.	[83]
Antioxidant effects
		The extract from methanol, acetone and 80% alcohol	The extraction with 80% alcohol has the strongest antioxidant activity on DPPH assay.	[84]
		The extract from water, ethanol, acetone, chloroform, ether, petroleum benzine	Acetone extract had the strongest antioxidant effect.	[85]
	LO₂ cells	Aqueous extract	The aqueous extract exerted a good antioxidant effect in DPPH assay with a 50% scavenging concentration at 0.153 mg/mL. Aqueous extract treatment reversed H₂O₂-induced activation of the MEK/ERK pathway and H₂O₂-induced inhibition of the P13-K/AKT/GSK3b pathway in LO₂ cells. This may be due to the improvement activity of the aqueous extract of H. diffusa on the antioxidant defense system.	[86]
		Twelve pure compounds isolated from H. diffusa	All compounds showed antioxidant effects on xanthine oxidase inhibition, xanthine-xanthine oxidase cytochrome c and TBA-MDA systems.	[33]
Anti-inflammatory effect
	Lipopolysaccharide-induced renal inflammation mice	Aqueous extract	The extract protected renal tissues, significantly suppressed the production of TNF-α, IL-1, IL-6 and MCP-1, as well as significantly promoted the production of IL-10 in serum and renal tissues.	[87]
	RAW 264.7 cells	Total flavonoids extract	The extract treatment on LPS-stimulated RAW 264.7 cells, reduced expression of iNOS, TNF-α, IL-6 and IL-1β, as well as suppressing phosphorylation of IκB p38, JNK and ERK1/2 in a concentration-dependent manner, indicating that the anti-inflammatory activity of total flavonoids had a close relationship with the NF-κB and MAPK signaling pathways.	[88]
Neuroprotective effect
	Rat cortical cells damaged by l-glutamate	Methanolic extract, five flavonoids and four O-acylated iridoid glycosides	All compounds exhibited significant neuroprotective activity in primary cultures of rat cortical cells damaged by l-glutamate.	[34]
Anti-fibrosis effect
	Ras oncogene-transformed R6 cells	Oleanolic acid	Oleanolic acid inhibits the growth of ras oncogene-transformed R6 cells. Oleanolic acid-mediated growth inhibition of transformed cells does not require direct cell–cell contact between normal and ras-transformed cells.	[89]

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3.1. Anti-Cancer Activity

3.1.1. Anti-Colorectal Cancer Activity

H. diffusa has been used as a major formula for the clinical treatment of colorectal cancer (CRC). In vitro, ethanol extract of H. diffusa treatment significantly suppresses proliferation and induced apoptosis of HT-29 cells, resulting in DNA fragmentation, loss of plasma membrane asymmetry, collapse of mitochondrial membrane potential, activation of caspase-9 and caspase-3, increase of the ratio of pro-apoptotic Bax to anti-apoptotic Bcl-2, reduction of the mRNA expression levels of cyclin D1, cyclin-dependent kinase 4 and B-cell lymphoma-2 (Bcl-2), upregulation of the expression levels of Bcl-2-associated X protein, prevention of G1–S progression, and reduction of mRNA expression of pro-proliferative PCNA, Cyclin D1 and CDK4. These results indicated that the anti-colorectal cancer cells effect of H. diffusa might be carried out via multiple approaches, such as the mitochondria-dependent pathway, IL-6/STAT3 pathway and cell cycle arrest [2,55,56,57,58]. The mechanism was also confirmed by animal experiments [58,59]. Meanwhile, the ethanolic extract of H. diffusa displayed an inhibition effect on CT-26 cells with inhibitory rates from 35.46% ± 3.59% to 71.84% ± 3.12% at different concentrations (0.06 mg/mL, 0.08 mg/mL, 0.10 mg/mL, 0.12 mg/mL, 0.14 mg/mL, 0.16 mg/mL, 0.18 mg/mL and 0.20 mg/mL) and showed a stronger inhibition effect with an increase of concentration [60]. Li et al. [61] revealed that the ethanolic extract treatment could overcome 5-fluorouracil resistance in HCT-8/5-FU cells by downregulating the expression of P-gp and ABCG2. In addition, 2-hydroxymethy-1-hydroxy anthraquinone (IC₅₀ 45 μM) and ursolic acid (IC₅₀ 71 μM)) isolated from H. diffusa exhibited inhibition effects on Caco-2 cell proliferation [5], and the mechanism of the inhibition effect for ursolic acid might include the cleavage of the Poly (ADP-ribose) Polymerase (PARP) [62].

3.1.2. Anti-Leukemia Activity

The anti-leukemia effects of both aqueous and ethanolic extracts of H. diffusa have been investigated in several cancer cell lines. H. diffusa aqueous extract treatment with 0.01–4150 μg/mL restrained the growth of the CEM cells by enhancing the expression of P53 in vitro [63] and influenced murine leukemia WEHI-3 cells, as well as promoting T- and B-cell proliferation in leukemic mice administrated with 16 and 32 mg/kg in vivo [64]. The ethanolic extract of H. diffusa could trigger an arrest of HL-60 cells at the G₀/G₁ phase and sub-G₁ population, provoke DNA condensation and DNA damage, but elevate the activities of caspase-3, caspase-8 and caspase-9, thus, inhibiting the cell proliferation of HL-60 cells with the half maximal inhibitory concentration (IC₅₀) value of 4.62 mg/mL [65,66]. Wang et al. [67] found that 2-hydroxy-3-methyl anthraquinone treatment (0–80 μM) could enhance apoptosis of U937 cells in a dose-dependent manner through the activation of p-p38MAPK and downregulation of p-ERK1/2. Further study verified it could alter the expression of Fas/FasL and activation of caspase-8, thus inducing THP-1 cell apoptosis [68].

3.1.3. Anti-Liver Cancer Activity

Li et al. [69] reported the inhibition of aqueous extract of H. diffusa on blood metastasis in H22 mice. The body and immune organs weights increased after administration with H. diffusa extract at three doses of 0.25, 0.5 and 1.0 mg/kg. In vitro, the aqueous extract of H. diffusa treatment (1.25–10 mg/mL) remarkably inhibited HepG2 cell proliferation in a dose-dependent manner, probably via the arrest of HepG2 cells at the G₀/G₁ phase and the induction of S phase delay [70]. Treatment with total flavones extract from H. diffusa could reverse the invasion of MHCC97-H cells in epithelial-mesenchymal transition induced by TGF-β1 at the dose of 200μg/mL, and the effect might be carried out by decreasing the level of E-cadherin protein and increasing the expression of vimentin protein [71]. Li et al. found that both 1,3-Dihydroxy-2-Methylanthraquinone (79 and 157 μmol/L) and ethyl acetate extract (100 and 200 μg/mL) induced apoptosis on HepG2 cells, resulting in upregulation of Bax, p53, Fas, FasL, p21 and cytoplasmic cytochrome C levels and caspase-3, -8, -9 proteases activities, while downregulation of Bcl-2, mitochondrial cytochrome C, cyclin E and CDK 2 in a dose-dependent manner [72]. Nine compounds from H. diffusa, namely, ethyl 13 (S)-hydroxy-chlorophyllide a, 2-methyl-3-methoxy anthraquinone, 2-hydroxymethyl anthraquinone, 2-hydroxy-3-methyl anthraquinone, 2-hydroxymethy-1-hydroxy anthraquinone, 2-hydroxy-1-methoxy anthraquinone, 2-hydroxy-3-methyl-1-methoxy anthraquinone, oleanolic acid and ursolic acid, have been researched for their anti-liver cancer effect within the concentration range from 1 to 200 μM. As a result, ursolic acid exhibited a strong inhibition of HepG2 cell survival (IC₅₀ 36.63 μM) [5]. Another study revealed that the inhibitory activity of 2-hydroxy-3-methyl anthraquinone (IC₅₀ 51 μM) and 2-hydroxy-1-methoxy anthraquinone (IC₅₀ 62 μM) might be achieved by activity against protein tyrosine kinases v-src and pp60src [38].

3.1.4. Anti-Lung Cancer Activity

Aqueous extract of H. diffusa treatment (0–200 μg/mL) showed a suppression effect on A549 and H1355 cells in a concentration-dependent manner. But this effect was not found in LLC cells [66]. Further, Shi et al. [38] confirmed that two compounds of 2-hydroxy-3-methyl anthraquinone (IC₅₀ 66 μM) and 2-hydroxy-1-methoxy anthraquinone (IC₅₀ 79 μM) from H. diffusa could induce apoptosis on SPC-1-A cells with a close relationship to the mitochondrial apoptotic pathway.

3.1.5. Anti-Breast Cancer Activity

Anthraquinones, iridoid glucosides, stigmasterols and alkaloid/flavonoid extracts were evaluated for anti-breast cancer using human breast cancer cell line MCF7. Dong et al. [73] found that the crude alkaloid/flavonoid extract, but not its three major components, possessed antitumor activity against the human breast cancer cell line MCF7. However, Liu et al. [74] observed that methyl anthraquinone from H. diffusa exhibited an inhibition effect on MCF7 cells with half maximal effective concentration (EC₅₀) of 18.62 ± 2.71 and 42.19 ± 3.84 μM for 24 and 48 h, respectively, and induced MCF-7 cells apoptosis via the Ca²⁺/calpain/caspase-4 pathway. Moreover, compounds of 2-hydroxy-3-methyl anthraquinon (IC₅₀ 57 μM) and 2-hydroxy-1-methoxy anthraquinone) (IC₅₀ 65 μM) inhibited protein tyrosine kinases v-src and pp60src and the growth of Bcap37 cells [38].

3.1.6. Anti-Cervical Tumor Activity

Zhang et al. [3] discovered that the aqueous extract of H. diffusa treated (0.5 g/kg bw) by intragastric administration on human cervical carcinoma xenograft in nude mice showed an inhibitory effect on cervical cancer cells and induced apoptosis of Hela cells. Meanwhile, anthraquinones, especially 2-hydroxymethy-1-hydroxy anthraquinone, showed a strong inhibitory effect on Hela cells with IC₅₀ 45 μM in vitro [5].

3.1.7. Anti-Prostate Cancer Activity

The potential anti-prostate cancer effect of H. diffusa, mainly the active compounds, has previously been provided on a variety of cell lines. 2-Methyl-3-methoxy anthraquinone (IC₅₀ 64.72–105.90 μM), 2-hydroxy-3-methyl anthraquinone (IC₅₀ 28.82–159.20 μM) and ursolic acid (IC₅₀ 22.33–36.08 μM) exhibited inhibitory effects on DU145, PC-3 and LNCaP cells [5]. 6-O-(E)-p-coumaroyl scandoside methyl esterand 10(S)-hydroxy pheophytin a showed an anti-proliferation effect on PC-3 cells in a dose-dependent manner from 0 to 60 μM, while 10(S)-hydroxy pheophytin a also showed a strong anti-proliferation effect on LNCaP cells with a significant effect, with an IC₅₀ value of 20 μM [52]. Hu et al. [51] isolated three cyclotides (DC 1-3) and studied their anti-prostate cancer effect. Thus, three cyclotides, especially DC 3 (1 mg/kg) showed inhibition against PC3, DU145 and LNCap cells. In addition, DC3 significantly inhibited development of the tumor in weight and size in the model of a prostate xenograft, and showed significant anti-cancer effect (p < 0.01) at a dose of 1 mg/kg, with 40.23% inhibition of the rate of tumor growth (weight).

3.1.8. Anti-Multiple Myeloma Activity

Up to now, the anti-multiple myeloma effect of H. diffusa has been proved in RPMI 8226 cells. The polysaccharides extracts (1, 2 and 3 mg/mL) [75], the compound of 2-hydroxymethyl-1-hydroxy anthraquinone (1–200 μM) [5], as well as H. diffusa injection (20, 40 and 60 μL/mL) [76], exhibited an inhibitory effect on RPMI 8226 cells growth in a dose-dependent manner.

3.1.9. Other Anti-Cancer Effects

Other anti-cancer effects have also been reported during these years. The ethanolic extract of H. diffusa (0–200 μg/mL) suppressed the proliferation of B16F10 cells in a dose-dependent manner [66]. The lipophilic extract (50 and 100 mg/kg) and crude polysaccharide (31.25 and 62.5 mg/kg) from H. diffusa showed anti-tumor activities on S-180 cells and a protective effect on chemotherapeutic damage [77]. H. diffusa injection could induce the apoptosis of MG-63 cells by increasing the Bax gene expression in a concentration-dependent manner from 50 to 400 μg/mL [78,79]. When it is used with cisplatin, the combined use exhibited a stronger inhibitory effect than the single agents. This might be due to its property of elevating the levels of Bax, Bad, caspase-3 and caspase-8 expression and decreasing the levels of Bcl-xl and Bcl-2 [80]. Meanwhile, Zhang et al. [4] found that the aqueous extract of H. diffusa (2–8 mg/mL) inhibited the growth of U87 cells in a dose-dependent manner by inducing mitochondrial apoptosis via the AKT/ERK pathways. Moreover, the compound, 4-vinyl phenol, was demonstrated to have anti-angiogenic activity in human endothelial cells of HUVEC (IC₅₀ 15.31 μg/mL) and HMEC_-1 (IC₅₀ 21.43 μg/mL), breast tumor-bearing BALB/c mice (0.2–2 mg/kg), C57BL/6 mice (20–100 μg/mL matrigel) and zebrafish embryo models (6.25–12.5 μg/mL matrigel), and this effect had a close relationship with the PI3K/AKT pathway [81].

3.2. Immunomodulatory Effect

Lin et al. [64] found that aqueous extract of H. diffusa (16 and 32 mg/kg) affected immune responses by promoting T- and B-cell proliferation in leukemic mice in WEHI-3-generated leukemia mice. Meanwhile, Kuo et al. [82] discovered that the ethanolic extract (16, 32 and 64 mg/kg) could also promote immune responses in normal BALB/c mice by promoting CD11b, CD19 and Mac-3 levels, increasing phagocytosis activity of macrophages obtained from the peritoneal cavity and increasing NK cell activity and B- and T-cell proliferation. The polysaccharides extracts (2.25, 4.5 and 9.0 mg/mL) could improve the clearance index, phagocytic index and the index of the thymus and spleen of immunosuppression mice [50]. When inmmunosuppressed mice were orally administrated total flavonoids of H. diffusa (15, 30 and 60 mg/kg), the levels of interleukin-2 (IL-2) and interferon-γ (INF-γ) were enhanced and the proliferation of T and B lymphocytes was increased, indicating the immunomodulatory effect of total flavonoids [83].

3.3. Antioxidant Effect

The aqueous, methanolic and 80% acetonic extracts were evaluated for antioxidant activity and the extraction from 80% alcohol (0.1–4.5 mg/mL) showed the strongest antioxidant activity, by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay [84]. Yu et al. [85] compared the antioxidant effects of aqueous, alcoholic, acetonic, chloroform, ether and petroleum benzene extracts from H. diffusa, and found that the acetonic extracts (0.03%–0.18%), especially the 0.12% acetone extract, had the strongest antioxidant effect, by determination of peroxide value. In addition, the aqueous extract (0.3–10 mg/mL) treatment could protect human hepatocyte cells from H₂O₂-induced cytotoxicity in a dose-dependent manner as the probable result of the improvement activity of the aqueous extract of H. diffusa on the antioxidant defense system by reversing H₂O₂-induced activation of the MEK/ERK pathway and H₂O₂-induced inhibition of the P13-K/AKT/GSK3β pathway in LO₂ cells [86]. The antioxidant effect of H. diffusa may be due to its compounds, like flavonoids and iridoids. Three flavonol glycosides (quercetin 3-O-sambubioside, kaempferol-3-O-[2-O-(E-6-O-feruloyl)-β-d- glucopyranosyl]-β-d-galactopyranoside and quercetin 3-O-sophoroside) and six known iridoid glycosides (asperuloside, asperulosidic acid methyl ester, (E)-6-O-p-methoxy cinnamoyl scandoside methyl ester, (E)-6-O-feruloyl scandoside methyl ester and (E)-6-O-coumaroyl scandoside methyl ester) were determined for their antioxidant effects on xanthine oxidase inhibition, xanthine-xanthine oxidase cytochrome c and TBA-MDA systems. In consequence, asperuloside (IC₅₀ 118.5 ± 0.70 μM) and kaempferol-3-O-(2-O-β-d-glucopyranosyl)-β-d-galactopyranoside (IC₅₀ 98.7 ± 0.16 μM) showed a minor anti-lipid peroxidation effect and quercetin di-glycosides exerted a remarkable antioxidant effect as superoxide anion scavengers [33].

3.4. Anti-Inflammatory Effect

The aqueous extract (5.0 g/kg bw) treatment exhibited an anti-inflammatory effect in lipopolysaccharide-induced renal inflammation of mice by significantly suppressing the production of tumor necrosis factor-α (TNF-α), IL-1, IL-6 and monocyte chemotactic protein 1 (MCP-1) in renal tissues, as well as significantly promoting the production of IL-10 in serum and renal tissues. Moreover, two main chemotypes, including eight flavonoids and four iridoid glycosides were found in renal tissues after H. diffusa treatment, indicating that the anti-inflammatory effect may be due to these constituents [87]. In vitro, Chen et al. found that the flavonoids extract treatment (50−100 μg/mL) on LPS-stimulated RAW 264.7 cells reduced expression of iNOS, TNF-α, IL-6 and IL-1β, as well as suppressing phosphorylation of IκB p38, JNK and ERK1/2 in a concentration-dependent manner, indicating that the anti-inflammatory activity of total flavonoids had a close relationship with the NF-κB- and MAPK-signaling pathways [88].

3.5. Others

Five flavonol glycosides (kaempferol-3-O-[2-O-(6-O-E-feruloyl)-β-d-glucopyranosyl]-β-d-galactopyranoside, quercetin-3-O-[2-O-(6-O-E-feruloyl)-β-d-glucopyranosyl]-β-d-galactopyranoside, quercetin-3-O-[2-O-(6-O-E-feruloyl)-β-d-glucopyranosyl]-β-d-glucopyranoside, kaempferol-3-O-(2-O-β-d-glucopyranosyl)-β-d-galactopyranoside and quercetin-3-O-(2-O-β-d-glucopyranosyl)-β-d-galactopyranoside) and four O-acylated iridoid glycosides (6-O-Z-p-methoxy cinnamoyl scandoside methyl ester, 6-O-E-p-methoxy cinnamoyl scandoside methyl ester, 6-O-Z-p-coumaroyl scandoside methyl ester and 6-O-E-p-coumaroyl scandoside methyl ester) isolated from H. diffusa exhibited a significant neuroprotective effect on l-glutamate-damaged rat cortical cells in the concentration from 0.1 to 10 μM; further, the structure–activity study proved di-OH in the B ring and an acyl substituent in flavonoids, a p-methoxy group in the aromatic ring and a trans double bond in the acyl moiety of acylated iridoid glycosides might be crucial for the biological response [34]. Wu et al. [89] found the inhibitory effect of oleanolic acid (2 and 8 μg/mL) isolated from H. diffusa against ras-transformed fibroblasts on R6 cells, and this inhibition might cause normal cells to secrete an inhibitory factor against the transformed cells, but did not require direct cell–cell contact.

4. Quality Control

Quality control of herbal medicines is necessary to ensure their stability, efficiency and safety. Modern analytical techniques provide simpler, more accurate and reliable methods for the quality control for H. diffusa. Besides the macroscopic and microscopic characters of H. diffusa [9], DNA sequence has become a powerful tool for the distinguishing H. diffusa from counterfeits, such as H. corymbosa and H. tenelliflora [8,12]. Chemical fingerprint is a comprehensive method accepted by the Food and Drug Administration, European Medicines Agency, and China Food and Drug Administration [90]. It can provide information about the types of compounds, as well as their relative ratios. A HPLC-MS fingerprint method was applied to 10 batches of H. diffusa materials from nine regions in China. The results showed that this method could differentiate samples from different geographical origins or processing methods [91]. Liang et al. [92] analyzed the chemical fingerprints of 17 batches of H. diffusa and found that the contents of asperuloside and (E)-6-O-p-coumaroyl scandoside methyl ester were quite different in samples collected from different habitats.

The quantitative analysis for the quality control of H. diffusa has mostly focused on the diversity of components by a series of analytical methods, such as UV, HPLC, TLC and LC/MS. Up to now, triterpenes (ursolic acidand oleanulic acid), iridoids ((E)-6-O-p-coumaroyl scandoside methyl ester), geniposidic acid, deacetyl asperulosidic acid methyl ester, asperuloside acid, asperulosideand (E)-6-O-feruloyl scandoside methyl ester), phenolic acid (p-coumaric acid and ferulic acid), flavonoids (quercetin, rutin, quercetin-3-O-β-d-glucopyranside, quercetin-3-O-sambubioside, kaempferol, kaempferol-3-O-β-d-glucopyranside), anthraquinones (2-hydroxy-3-methoxy-7-methyl anthraquinone and 2-hydroxy-1-methoxy anthraquinone), polysassharides and one miscellaneous compound have been quantified as mark compounds for the quality control of H. diffusa. However, there were wide variations in the contents of these compounds, caused by samples from different sources and different collecting times (Table 3). Therefore, it is very urgent that a comprehensive method for ensuring the quality of H. diffusa be established.

Table 3.

Quantitative analysis for the quality control of H. diffusa.

Analytes	Method	Results	Reference
Deacetyl asperulosidic acid methyl ester	HPLC	The contents of deacetyl asperulosidic acid methyl ester of 22 batches were from 0.31 to 3.34 mg/g.	[93]
Oleanolic acid	TLC	The contents of oleanolic acid of 3 batches were from 1.63% to 1.72%	[94]
Isoscutellarein	HPLC	The contents of isoscutellarein have a close relationship with the collecting times and were also different in leaves (1.11–2.72 mg/g) and stem (0.35–0.94 mg/g).	[95]
p-Coumaric acid	HPLC	The contents of p-coumaric acid in the injection of H. diffusa from four manufacturers ranged from 0.34 to 0.49 mg/mL.	[96]
p-Coumaric acid	HPLC	The contents of p-coumaric acid of 13 batches were from 0.46 to1.88 mg/mL	[97]
3,4-Dihydroxy methyl benzoate	HPLC	The contents of 3,4-dihydroxy methyl benzoate of 8 batches were from 40.8 to 87.0 μg/g.	[98]
Polysassharides	UV	Polysassharides have been determined by the phenol-sulfuric acid method by spectrophosured at 490 nm, and the content was 15.10%.	[99]
Ursolic acid Oleanolic acid	HPLC	Six batches have been determined with the contents of 1.75–3.37 mg/g for ursolic acid and 0.50–0.80 mg/g for oleanolic acid, indicating that the ursolic acid and oleanolic acid content in the samples from different sources were significantly different.	[100]
Ursolic acid Oleanolic acid	HPLC	The contents of ursolic acid and oleanolic acid have a close relationship with the collecting time. The range of contents was 1.17–3.75 and 0.19–0.96 mg/g for ursolic acid and oleanolic acid, respectively.	[101]
Ursolic acid Oleanolic acid	HPLC	The contents of ursolic acid and oleanolic acid were 0.51%–0.58% and 0.11%–0.14%, respectively. And the contents of the whole herb were slightly lower than those of the overground part for both of the two compounds.	[102]
Ursolic acid Oleanolic acid	HPLC-MS/MS	The contents of ursolic acid and oleanolic acid for 10 batches were 0.15%–0.65% and 0.06%–0.17%, respectively.	[103]
2-Hydroxy-3-methoxy-7-methyl anthraquinone 2-Hydroxy-1-methoxy anthraquinone	HPLC	The contents were 0.16–0.51 and 0.22–0.49 mg/g for 2-hydroxy-3-methoxy-7-methyl anthraquinone and 2-hydroxy-1-methoxyanthraquinone, respectively.	[104]
Asperuloside E-6-O-p-Coumaroyl scandoside methyl ester E-6-O-p-Coumaroyl scandoside methyl ester-10-methyl ether	HPLC	The contents of asperuloside, E-6-O-p-coumaroyl scandoside methyl ester and E-6-O-p-coumaroyl scandoside methyl ester-10-methyl ether have been determined in twenty-three batches. The result was that the contents of the compounds were significantly varied among the different samples. The concentration ranges were 0–7.885, 1.104–7.159 and 0–1.795 mg/g for asperuloside, E-6-O-p-coumaroyl scandoside methyl ester and E-6-O-p-coumaroyl scandoside methyl ester-10-methyl ether, respectively.	[105]
3,4-Dihydroxy methyl benzoate p-Coumaric acid Ferulic acid (E)-6-O-p-Coumaroyl scandoside methyl ester	HPLC	Four compounds have been quantified in the injection of H. diffusa with contents of 2.25–2.63, 7.02–7.15, 0.96–1.17 and 7.16–7.33 g/L for 3,4-dihydroxy methyl benzoate, p-coumaric acid, ferulic acid and (E)-6-O-p-coumaroyl scandoside methyl ester, respectively.	[106]
Geniposidic acid Ursolic acid Quercetin p-Coumaric acid	CE	Four compounds have been quantified in the injection of H. diffusa with contents of 1.004, 1.182, 0.110 and 0.067 mg/g for ursolic acid, geniposidic acid, quercetin and p-coumaric acid, respectively.	[107]
Asperuloside acid Asperuloside (E)-6-O-Feruloyl scandoside methyl ester (E)-6-O-p-Coumaroyl scandoside methyl ester Scandoside methyl ester	HPLC	The contents were 1.57–5.93, 1.45–3.86, 1.82–3.23, 1.54–3.82 and 1.49–4.11 mg/g for asperuloside acid, asperuloside, (E)-6-O-feruloyl scandoside methyl ester, (E)-6-O-p-coumaroyl scandoside methyl ester and scandoside methyl ester, respectively, and they were very different in different batches.	[108]
Quercetin-3-O-sambubioside Quercetin-3-O-β-d-glucopyranside Kaempferol-3-O-β-d-glucopyranside Rutin Quercetin Kaempferol	HPLC	Six compounds from eight batches of H. diffusa have been quantified with contents of 1.36–6.32, 0.98–10.23, 0.79–7.98, 4.92–15.78, 0.52–1.72 and 0.75–2.15 mg/g for quercetin-3-O-sambubioside, quercetin-3-O-β-d-glucopyranside, kaempferol-3-O-β-d-glucopyranside, rutin, quercetin and kaempferol, respectively, indicating that the contents for these compounds were quite different from different regions.	[109]
Desacetyl asperulosidic acid Asperuloside Aesculetin Coumaric acid Ferulic acid Quercetin Kaempferol	HPLC	Seven compounds from six batches of H. diffusa have been quantified with contents of 42.48 ± 1.43, 63.76 ± 1.01, 1765 ± 0.69, 881.9 ± 0.74, 86.99 ± 1.65, 1395 ± 0.731 and 902.2 ± 0.82 μg/g for desacetyl asperulosidic acid, asperuloside, aesculetin, coumaric acid, ferulic acid, quercetin, kaempferol, respectively.	[110]

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5. Pharmacokinetics

The investigations about pharmacokinetics of H. diffusa are very scarce. After oral administration of H. diffusa in lipopolysaccharide-induced renal inflammation in mice, most compounds, including flavonoids, iridoid glycosides and anthraquinone, were found in plasma, and 12 compounds (eight flavonoids and four iridoid glycosides) were found in kidney, determined by UPLC-Q-TOF-MS/MS. The results indicated that flavonoids, iridoids and anthraquinones might be responsible for the protective effect of H. diffusa on renal inflammation [87]. Liu et al. [111] found that p-coumaric acid was a major metabolite of (E)-6-O-p-coumaroyl scandoside methyl ester in rat plasma after oral administration of a dose of 20 mg/kg. Compared with direct administration of p-coumaric acid, the absorption and elimination of p-coumaric acid were slower with administration of (E)-6-O-p-coumaroyl scandoside methyl ester. This was also confirmed by Yan et al. at 2011 [112]. Moreover, Ganbold et al. [62] investigated the bioavailability of H. diffusa by production of post-absorption samples using the Caco-2 cell model and confirmed that the decoction has good permeability (Papp = 3.575 × 10⁻⁶ cm/s) in vitro with no cytotoxic effect.

6. Conclusions

Although H. diffusa has been used in China for thousands of years as a heat-clearing and detoxifying medicine, it has become popular for its anti-cancer effect, especially in the Taiwan district. Modern research on H. diffusa has provided much evidence for its anti-cancer effect using in vitro and in vivo experiments and has tried to clarify the mechanism of its action. Meanwhile, its other activities, such as anti-oxidant, anti-inflammatory, anti-fibroblasts, immunomodulatory and neuroprotective effects, have been reported. The achievement of these therapeutic effects is due to the chemical composition of H. diffusa. One hundred and seventy-one compounds have been reported, including iridoids, flavonoids, anthraquinones, phenolic acids and their derivatives, sterols, triterpenes, polysaccharides, cyclotides, coumarins, alkaloids and volatile oils. Among these constituents, iridoids, flavonoids and anthraquinones are three main ingredients and may play an essential role in its activities. However, there is no official quality standard for the quality control of H. diffusa. The contents of bioactive compounds are significantly different in the samples from different sources and different collecting times. So, a feasible and reliable approach is urgently needed in considering the botanical origin and bioactive effects. Moreover, a relatively small number of pharmacokinetics studies have been summarized, and, therefore, it is difficult to evaluate the function of H. diffusa in the human body. Altogether, this review gives comprehensive information about H. diffusa and provides evidence for its clinical application and further development.

Acknowledgments

The project was financially supported by the National Natural Science Foundation of China (81503376) and Guangdong Natural Science Foundation (2014A030310280).

Author Contributions

Rui Chen wrote the Introduction, Phytochemistry and Pharmacology sections; Jingyu He wrote the Quality control and Pharmacokinetics sections; Xueli Tong sorted out the references; Lan Tang performed the English editing; Menghua Liu designed this review and wrote the Conclusions section.

Conflicts of Interest

The authors declare no conflict of interest.

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Compounds	R₁	R₂
45. Quercetin	OH	H
46. Rutin	OH	rutinose
47. Quercetin-3-O-β-d-glucopyranside	OH	β-d-Glc
48. Quercetin-3-O-β-d-galactopyranoside	OH	β-d-Gal
49. Quercetin-3-O-(2-O-glucopyranosyl)-β-d-glucopyranside	OH	β-d-Glc-(1→2)-d-Glc
50. Quercetin-3-O-(2-O-glucopyranosyl)-β-d-galactopyranoside	OH	β-d-Glc-(1→2)-d-Gal
51. Quercetin-3-O-sambubioside	OH	β-d-Xyl-(1→2)-d-Glc
52. Quercetin-3-O-[2-O-(6-O-E-ferloyl)-β-d-glucopyranosyl]-β-d-galactopyranoside	OH	6′-O-E-feruloyl-β-d-Glc-(1→2)-d-Gal
53. Quercetin-3-O-[2-O-(6-O-E-feruloyl)-β-d-glucopyranosyl]-β-d-glucopyanoside	OH	6′-O-E-feruloyl-β-d-Glc-(1→2)-d-Glc
54. Quercetin-3-O-[2-O-(6-O-E-sinapoyl)-β-d-glucopyranosyl]-β-d-glucopyanoside	OH	6′-O-E-sinapoyl-β-d-Glc-(1→2)-d-Glc
55. Quercetin-3-O-[2-O-(6-O-E-sinapoyl)-β-d-glucopyranosyl]-β-d-galactopyranoside	OH	6′-O-E-sinapoyl-β-d-Glc-(1→2)-d-Gal
56. Kaempferol	H	H
57. Kaempferol-3-O-β-d-glucopyranside	H	β-d-Glc
58. Kaempferol-3-O-β-d-galactopyranoside	H	β-d-Gal
59. Kaempferol-3-O-(2-O-β-d-glucopyranosyl)-β-d-galactopyranoside	H	β-d-Glc-(1→2)-d-Gal
60. Kaempferol-3-O-(6-O-α-l-rhamnosyl)-β-d-glucopyranside	H	α-l-Rha-(1→6)-β-d-Glc
61. Kaempferol-3-O-[2-O-(6-O-E-ferloyl)-β-d-glucopyranosyl]-β-d-glucopyranside	H	6′-O-E-feruloyl-β-d-Glc-(1→2)-d-Glc
62. Kaempferol-3-O-[2-O-(6-O-E-feruloyl)-β-d-glucopyranosyl]-β-d-galactopyranoside	H	6′-O-E-feruloyl-β-d-Glc-(1→2)-d-Gal

Compounds	R₁	R₂	R₃	R₄	R₅	R₆
63. 2-Methyl-3-methoxy anthraquinone	H	CH₃	OCH₃	H	H	H
64. 2-Hydroxy-1,3-dimethoxy anthraquinone	OCH₃	OH	OCH₃	H	H	H
65. 2-Hydroxy-3-methyl-1-methoxy anthraquinone	OCH₃	OH	CH₃	H	H	H
66. 2-Hydroxy-3-methyl-4-methoxy anthraquinone	H	OH	CH₃	OCH₃	H	H
67. 2-Hydroxy-7-methyl-3-methoxy anthraquinone	H	OH	OCH₃	H	H	CH₃
68. 2-Hydroxy-1-methoxy-3-methyl anthraquinone	OCH₃	OH	CH	H	H	H
69. 2-Hydroxy-3-methyl anthraquinone	H	OH	CH₃	H	H	H
70. 2-Hydroxy-1-methoxy anthraquinone	OCH₃	OH	H	H	H	H
71. 2-Hydroxy-4-methoxy anthraquinone	H	OH	H	OCH₃	H	H
72. 2-Hydroxy-3-methoxy-7-methyl anthraquinone	H	OH	OCH₃	H	H	CH₃
73. 2-Hydroxy-6-methyl anthraquinone	H	OH	H	H	CH₃	H
74. 2-Hydroxy-3-methoxy-6-methyl anthraquinone	H	OH	OCH₃	H	CH₃	H
75. 2,7-Dihydroxy-3-methyl anthraquinone	H	OH	CH₃	H	H	OH
76. 3-Hydroxy-2-methyl anthraquinone	H	CH₃	OH	H	H	H
77. 3-Hydroxy-2-methyl-4-methoxy anthraquinone	H	CH₃	OH	OCH₃	H	H
78. 2,3-Dimethoxy-6-methyl anthraquinone	H	OCH₃	OCH₃	H	CH₃	H
79. 1,3-Dihydroxy-2-methyl anthraquinone	OH	CH₃	OH	H	H	H
80. 1,7- Dihydroxy-6-methoxy-2-methyl anthraquinone	OH	CH₃	H	H	OCH₃	OH
81. 3-Hydroxy-2-methyl-4-methoxy anthraquinone	H	CH₃	OH	OCH₃	H	H
82. 2,6-Dihydroxy-3-methyl-4-methoxy anthraquinone	H	OH	CH₃	OCH₃	OH	H
83. 2,6-Dihydroxy-1-methoxy-3-methyl anthraquinone	OCH₃	OH	CH₃	H	OH	H
84. 1-Hydroxy-4-methoxy anthraquinone	OH	H	H	OCH₃	H	H
85. 2-hydroxymethyl-1-hydroxy anthraquinone	OH	CH₂OH	H	H	H	H
86. 2-hydroxymethyl anthraquinone	H	CH₂OH	H	H	H	H

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The Hedyotis diffusa Willd. (Rubiaceae): A Review on Phytochemistry, Pharmacology, Quality Control and Pharmacokinetics

Rui Chen

Jingyu He

Xueli Tong

Lan Tang

Menghua Liu

Roles

Abstract

1. Introduction

2. Phytochemistry

Table 1.

2.1. Iridoids and Triterpenes

Figure 1.

2.2. Flavonoids

Figure 2.

2.3. Anthraquinones

Figure 3.

2.4. Phenolic Acids and Their Derivatives

Figure 4.

2.5. Polysaccharides

2.6. Essential Oils

2.7. Cyclotides

2.8. Miscellaneous

Figure 5.

3. Pharmacology

Table 2.

3.1. Anti-Cancer Activity

3.1.1. Anti-Colorectal Cancer Activity

3.1.2. Anti-Leukemia Activity

3.1.3. Anti-Liver Cancer Activity

3.1.4. Anti-Lung Cancer Activity

3.1.5. Anti-Breast Cancer Activity

3.1.6. Anti-Cervical Tumor Activity

3.1.7. Anti-Prostate Cancer Activity

3.1.8. Anti-Multiple Myeloma Activity

3.1.9. Other Anti-Cancer Effects

3.2. Immunomodulatory Effect

3.3. Antioxidant Effect

3.4. Anti-Inflammatory Effect

3.5. Others

4. Quality Control

Table 3.

5. Pharmacokinetics

6. Conclusions

Acknowledgments

Author Contributions

Conflicts of Interest

References

ACTIONS

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RESOURCES

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