Table 1.
Precursor/product ion pairs and parameters for MRM of compounds used in this study.
| No. | Compound | tR (min) | [M − H]− (m/z) | MRM Transitions (Precursor→Product) | Fragmentor (V) | Collision Energy (eV) |
|---|---|---|---|---|---|---|
| 1 | Fumaric acid | 1.42 | 114.9 | 114.9→71.0 | −55 | −11 |
| 2 | Caffeic acid | 3.34 | 179.0 | 179.0→134.9 | −52 | −23 |
| 3 | Catalpol | 3.59 | 361.0 | 361.0→198.9 | −76 | −11 |
| 4 | Aucubin | 5.02 | 345.1 | 345.1→183.1 | −65 | −13 |
| 5 | p-Coumaric acid | 5.35 | 163.0 | 163.0→118.9 | −56 | −19 |
| 6 | Ferulic acid | 6.38 | 192.9 | 192.9→133.9 | −54 | −24 |
| 7 | Harpagide | 6.42 | 363.1 | 363.→182.9 | −90 | −21 |
| 8 | Cinnamic acid | 10.57 | 146.9 | 146.9→103.0 | −64 | −16 |
| 9 | p-Methoxycinnamic acid | 13.38 | 177.0 | 177.0→133.0 | −52 | −16 |
| 10 | Verbascoside | 14.19 | 623.3 | 623.3→161.0 | −116 | −57 |
| 11 | Angoroside C | 15.34 | 783.4 | 783.4→175.1 | −129 | −55 |
| 12 | Harpagoside | 17.34 | 493.4 | 493.4→147.1 | −65 | −31 |