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. 2016 Oct 21;21(10):1378. doi: 10.3390/molecules21101378

Table 1.

Effect of bovine colostrums protein on the oxidation of 2′-dG to 8-OH-2′-dG induced by Fenton reaction.

Addition to RM * 8-OH-2′-dG (μg/mL)
Whey Casein Skimmed Milk
PBS buffer 0.36 e,** 0.36 b,** 0.36 d,**
15 mM Ascorbic acid 10.03 a 10.03 a 10.03 a
1 mg/mL 0.46 b,c 0.33 b 1.45 b,c
2 mg/mL 0.33 d 0.24 b 1.46 b,c
4 mg/mL 0.35 c,d 0.32 b 1.38 b,c
6 mg/mL 0.24 d 0.38 b 1.24 c
8 mg/mL 0.55 b 0.38 b 1.36 b,c
10 mg/mL 0.33 d 0.41 b 1.49 b

* RM (reaction mixture) containing 0.5 mM 2′-dG, 1.3 mM FeCl2, 50 mM H2O2, 6.5 mM EDTA, 15 mM ascorbic acid, and 0.1 M phosphate buffer (pH 7.4) was shaken at 37 °C for 30 min. ** Values with different superscripts are significantly different (p < 0.05).