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(a) Gel retardation assay of nanocomplexes. 1. Naked pDNA; 2. pDNA/PEI (1:8); 3. pDNA/PEI/HA (1:8:5); 4. pDNA/PEI/HA (1:8:10); and 5. pDNA/PEI/HA (1:8:20); (b) Viability of hASCs treated with each nanocomplex formulation as measured by MTS assay (n = 3). hASCs were incubated with various nanocomplexes for 24 h and cell viability was measured. ** p < 0.01 vs. control. n.s = not significant vs. control.
