Table 3.
Enzymatic screening of peracetylated nucleosides (1, 5) by immobilized protease N.
| Substrate | Support | t (h) | Vh | Conversion (%) | Product (Yield %) 5′-OH |
|---|---|---|---|---|---|
| 1 | Native enzyme | 22 | 0.52 | 100 | 1a (93) |
| 1 | Agarose CNBr | 15 | 0.14 | 99 | 1a (94) |
| 1 | Agarose glutaraldehyde | 24 | 0.14 | 81 | 1a (69) |
| 5 | Native enzyme | 48 | 0.16 | 92 | 5a (84) |
| 5 | Agarose CNBr | 48 | 0.05 | 94 | 5a (66) |
| 5 | Agarose glutaraldehyde | 216 | 0.06 | 88 | 5a (54) |
Experimental conditions: 10% CH3CN in 25 mM phosphate buffer pH 7, immobilized protease N 2 IU, reaction volume 10 mL, substrate: 10 mM, r.t.