Table 3.
Primers | Sequence (5′ to 3′) | Base Numbers | Purification Method | |
---|---|---|---|---|
β-actin | Forward | AAGGCTGTGGGCAAGG | 16 | HAP * |
β-actin | Reverse | TGGAGGAGTGGGTGTCG | 17 | HAP |
MDR1 | Forward | GGAGCGGTTCTACGA | 15 | HAP |
MDR1 | Reverse | ACGATGCCCAGGTGT | 15 | HAP |
MRP1 | Forward | GTCGGAACAAGTCGTGCCTG | 20 | HAP |
MRP1 | Reverse | CAAAGCCTCCACCTCCTCA | 19 | HAP |
LRP | Forward | GGCTCCTTCCGCTACGT | 17 | HAP |
LRP | Reverse | GCCGAGACCGCTCAATAC | 18 | HAP |
GST-π | Forward | CTGGAAGGAGGAGGTGGTG | 19 | HAP |
GST-π | Reverse | GACGCAGGATGGTATTGGAC | 20 | HAP |
PKC | Forward | CCCAAACATTGACAAATCCTAACC | 24 | HAP |
PKC | Reverse | CAACCAAGGAGGGTACCAGATG | 22 | HAP |
Topo II β | Forward | GAAACGGAATCCTTGGTCAGAT | 22 | HAP |
Topo II β | Reverse | TTTCGGCTGCTGCTCTCCTA | 20 | HAP |
* HAP (High affinity purification), is a new purification method of DNA which purity is up to 98%.