Table A3.
Residues detected as putative HS on mtGS PPI and their classification as HS, WS or NS. In the columns “A” to “L”, each one referring to a GS chain, an x indicates that the residue on that monomer was detected as a potential HS (buried area ≥ 40 Å2). If the residue was detected in at least 10 monomers, ΔΔGbind was determined by cASM and his classification (HS/WS/NS) is presented in the last column.
| Resid | A | B | C | D | E | F | G | H | I | J | K | L | Tested | Classif |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 20 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | NS |
| 31 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | NS |
| 32 | x | x | x | No | ||||||||||
| 33 | x | x | No | |||||||||||
| 34 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| 35 | x | x | x | x | x | x | x | x | x | x | x | Yes | NS | |
| 36 | x | x | x | x | x | x | x | x | x | x | No | |||
| 40 | x | x | x | No | ||||||||||
| 54 | x | x | x | x | x | x | x | x | x | No | ||||
| 59 | x | x | x | x | x | No | ||||||||
| 64 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| 65 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | NS |
| 66 | x | x | No | |||||||||||
| 68 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | NS |
| 84 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| 99 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | NS |
| 141 | x | x | x | x | x | No | ||||||||
| 142 | x | x | x | x | x | x | x | x | x | x | x | Yes | WS | |
| 143 | x | x | x | x | x | No | ||||||||
| 144 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | WS |
| 146 | x | No | ||||||||||||
| 147 | x | x | x | x | x | x | No | |||||||
| 149 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | WS |
| 151 | x | x | x | x | x | x | x | x | x | x | x | Yes | NS | |
| 152 | x | x | x | x | x | x | x | x | x | No | ||||
| 153 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| 154 | x | x | x | x | x | x | x | x | x | x | x | Yes | NS | |
| 161 | x | x | x | x | x | x | x | No | ||||||
| 162 | x | x | No | |||||||||||
| 164 | x | x | x | x | x | x | x | x | x | x | No | |||
| 175 | x | x | x | x | x | x | x | No | ||||||
| 176 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| 178 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | WS |
| 179 | x | x | x | x | x | No | ||||||||
| 181 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| 186 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | WS |
| 187 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | WS |
| 189 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | WS |
| 194 | x | No | ||||||||||||
| 196 | x | x | x | x | x | x | x | x | x | x | x | Yes | NS | |
| 197 | x | No | ||||||||||||
| 199 | x | x | x | x | No | |||||||||
| 200 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | NS |
| 203 | x | x | x | x | x | x | x | x | x | No | ||||
| 207 | x | x | No | |||||||||||
| 212 | x | x | No | |||||||||||
| 213 | x | x | x | No | ||||||||||
| 214 | x | x | x | x | x | No | ||||||||
| 215 | x | x | x | x | x | x | x | x | x | x | No | |||
| 247 | x | x | x | x | x | No | ||||||||
| 250 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| 251 | x | x | x | x | x | x | No | |||||||
| 254 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| T261 | x | x | x | x | x | No | ||||||||
| M263 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| P265 | x | x | No | |||||||||||
| F268 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | WS |
| N325 | x | x | x | No | ||||||||||
| Y327 | x | No | ||||||||||||
| K328 | x | x | x | x | x | x | x | x | x | x | x | Yes | HS | |
| V331 | x | x | x | No | ||||||||||
| R345 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| N346 | x | No | ||||||||||||
| R347 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| R352 | x | x | No | |||||||||||
| I355 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | WS |
| T356 | x | No | ||||||||||||
| D404 | x | x | x | x | x | x | x | x | No | |||||
| T421 | x | x | x | x | No | |||||||||
| Q422 | x | x | x | x | x | x | x | x | x | x | No | |||
| S424 | x | No | ||||||||||||
| E457 | x | No | ||||||||||||
| E459 | x | x | x | No | ||||||||||
| V463 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| N464 | x | x | x | x | x | x | x | x | x | x | No | |||
| I465 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | NS |
| R466 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| H468 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| Y470 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | WS |
| E471 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| F472 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| L474 | x | x | x | x | x | x | x | x | x | x | x | Yes | WS | |
| Y475 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| Y476 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | HS |
| D477 | x | x | x | x | x | x | x | x | x | x | x | x | Yes | NS |
| V478 | x | x | x | x | x | x | x | x | x | x | x | x | No |