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. 2016 Jan 6;21(1):59. doi: 10.3390/molecules21010059

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© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Proposed biosynthetic machinery of haliamide (1). (a) Genetic organization of the haliamide biosynthetic gene cluster (hla, 21.7 kbp) and a biosynthetic pathway for 1; (b) Decarboxylative chain termination at the final step leading to the formation of the terminal olefin at C-9 and C-10. Abbreviations: A, adenylation domain; ACP, acyl carrier protein; AT, acyl transferase; C, condensation domain; DH, dehydratase; ER, enoyl reductase; KR, ketoreductase; KS, ketosynthase; PCP, peptide carrier protein; ST, sulfotransferase; TE, thioesterase.