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. 2016 Jun 1;21(6):721. doi: 10.3390/molecules21060721

Figure 5.

Figure 5

Effect of CSE (0.01–1 mg/mL), CGA (6.88 μg/mL), CAF (19.86 μg/mL) and Vit C (0.1 μg/mL) against oxidative damage induced by t-BOOH 0.5 mM. Cells were pre-treated with CSE, CGA, CAF and Vit C for 24 h, incubated with the 2′,7′-dichloro-dihydro-fluorescein diacetate (DCFH-DA) probe for 30 min and further exposed to 0.5 mM t-BOOH for 1 h. Then, the fluorescence of intracellular ROS was measured. Data represent the means ± SEM of 18 samples per condition. Different letters denote statistically-significant differences between all treatments (p < 0.05).