Table 9.

Quantitative apoptosis assay of A549 using Annexin-V/PI dual staining method. The percentage of viable cells, early apoptotic cells, late apoptotic cells, and necrotic cells are presented as mean ± SD.

Group/Concentration	Q1	Q2	Q3	Q4
MCF-7
Negative control	5.95 ± 007	16.80 ± 0.28	76.25 ± 1.77	0.25 ± 0.35
Positive control 3 µM	27.50 ± 0.35	62.5 ± 3.53	12.5 ± 0.35	1.65 ± 0.49
Compound 2c
3 µM	9.10 ± 0.77	67.1 ± 0.64	22.9 ± 0.78	0.8 ± 0.14
1.5 µM	25.5 ± 0.23	48.5 ± 0.21	25.0 ± 1.41	1.0 ± 0.14
0.75 µM	34.5 ± 0.71	31.2 ± 1.10	32.2 ± 0.31	1.7 ± 0.84
HeLa
Negative control	27.45 ± 0.78	31.80 ± 0.25	46.00 ± 0.56	0.05 ± 0.07
Positive control 3 µM	40.40 ± 1.09	58.5 ± 0.21	1.55 ± 0.64	0.95 ± 0.07
Compound 3c
10 µM	52.7 ± 0.92	24.6 ± 0.28	22.5 ± 0.35	0.1 ± 0.03
1 µM	50.15 ± 0.34	39.155 ± 1.20	12.85 ± 1.22	0.05 ± 0.01
0.1 µM	39.15 ± 1.20	49.8 ± 0.25	9.95 ± 0.10	0.15 ± 0.07
A549
Negative control	52.8 ± 0.28	30.8 ± 0.28	16.45 ± 0.64	0.15 ± 0.07
Positive control 3 µM	70.0 ± 1.41	28.4 ± 0.84	0.38 ± 0.07	0.0 ± 0.00
Compound 7a
10 µM	26.9 ± 0.10	4.1 ± 0.78	68.9 ± 0.78	0.1 ± 0.28
5 µM	39.7 ± 0.42	9.8 ± 1.13	51.0 ± 1.41	0.05 ± 0.07
2.5 µM	49.85 ± 0.21	15.2 ± 1.70	36.95 ± 0.43	0.15 ± 0.07