Table 1.
Table represents the quantification data of trastuzumab signature peptide (IYPTNGYTR) in human serum batch; calibration curve (CC) extends from 5 µg/mL to 500 µg/mL; their corresponding accuracy percentage and R2 = 0.99 was observed.
| Sample Name (CC) | Analyte Peak Area | Analyte Retention Time (RT) (min) | Internal Standard (IS) Peak Area | IS RT (min) | Area Ratio | Analyte Conc (µg/mL) | Calculated Conc (µg/mL) | Accuracy (%) |
|---|---|---|---|---|---|---|---|---|
| STD_8 | 2201571 | 4.476 | 1269609 | 4.471 | 1.734 | 501.6 | 526.911 | 105 |
| STD_7 | 1764292 | 4.563 | 1184001 | 4.557 | 1.49 | 451.44 | 452.73 | 100.3 |
| STD_6 | 1523150 | 4.528 | 1249879 | 4.523 | 1.219 | 375.598 | 370.177 | 98.6 |
| STD_5 | 1075654 | 4.46 | 1216198 | 4.456 | 0.884 | 251.651 | 268.549 | 106.7 |
| STD_4 | 289982 | 4.537 | 1226623 | 4.534 | 0.236 | 75.495 | 71.487 | 94.7 |
| STD_3 | 101021 | 4.534 | 1244545 | 4.531 | 0.081 | 25.668 | 24.281 | 94.6 |
| STD_2 | 44585 | 4.514 | 1299658 | 4.511 | 0.034 | 10.267 | 10.029 | 97.7 |
| STD_1 | 23549 | 4.501 | 1264843 | 4.5 | 0.019 | 5.134 | 5.259 | 102.4 |