Table 3.
Precision and accuracy of LC-MS/MS method.
| Parameters | Lower-Limit of Quantitation (LOQ QC) | Low-Quality Control (LQC) | Medium Quality Control (MQC) | High Quality Control (HQC) |
|---|---|---|---|---|
| Calculated concentration (µg/mL) | 5.416 | 16.237 | 223.708 | 397.879 |
| 5.421 | 16.366 | 252.093 | 462.144 | |
| 5.374 | 13.4 | 216.874 | 409.452 | |
| 4.857 | 15.199 | 264.181 | 449.311 | |
| 5.569 | 13.163 | 269.631 | 400.535 | |
| 5.94 | 16.551 | 229.291 | 356.099 | |
| Mean | 5.4295 | 15.1527 | 242.6297 | 412.5700 |
| Standard deviation (SD±) | 0.34962 | 1.52552 | 22.28179 | 38.38449 |
| Precision (CV%) | 6.4 | 10.1 | 9.2 | 9.3 |
| Nominal value (µg/mL) | 5.326 | 15.218 | 249.480 | 396.000 |
| Accuracy% | 101.9 | 99.6 | 97.3 | 104.2 |
| Number of samples (N) | 6 | 6 | 6 | 6 |