Table 3.
Amino Acids | PC1 | PC2 | PC3 | PC4 |
---|---|---|---|---|
Threonine | 129.97 ± 2.6 d | 141.18 ± 1.7 c | 167.03 ± 3.6 b | 289.078 ± 2.64 a |
Valine | 140.51 ± 3.4 d | 186.62 ± 3 c | 228.35 ± 3.21 b | 269.18 ± 3.3 a |
Methionine | 36.32 ± 4.3 d | 44.6 ± 2.6 c | 93.45 ± 2 b | 127.63 ± 1.2 a |
Isoleucine | 98.85 ± 1.1 d | 134.41 ± 2.7 c | 142.04 ± 2.5 b | 251.11 ± 2 a |
Leucine | 94.48 ± 2.16 d | 123.59 ± 2 c | 218.31 ± 3.5 b | 293.87 ± 2 d |
Phenylalanine | 66.04 ± 4.5 d | 74.24 ± 1.1 c | 119.13 ± 0.9 b | 168.9 ± 3 a |
Lysine | 52.28 ± 1.2 d | 60.67 ± 4.6 c | 102.46 ± 2.2 b | 157.9 ± 4 a |
Histidine | 18.49 ± 1.3 a | ND c | 14.59 ± 3.8 b | ND c |
Aspartic acid | 292.98 ± 3.6 b | 275.4 ± 3.01 c | 253.26 ± 1.7 d | 662.81 ± 1.1 a |
Alanine | 458.46 ± 1.3 b | 522.44 ± 5.1 a | 436.25 ± 1.6 c | 304.55 ± 2.2 d |
Cystine | 24.14 ± 1.09 d | 64.53 ± 3.1 b | 73.93 ± 4.3 a | 42.17 ± 2.01 c |
Glutamc acid | 932.74 ± 1.2 a | 444.02 ± 3 b | 264.24 ± 4.4 d | 369.56 ± 2.0 c |
Glycine | 51.31 ± 3.2 c | 139.61 ± 1.7 a | 126.54 ± 2.2 b | 140.77 ± 4.03 a |
Sarcosine | 101.44 ± 4.2 a | 55.96 ± 3.0 b | 101.17 ± 2.1 a | 84.94 ± 2.1 c |
Tyrosine | 94.39 ± 2.9 d | 118.34 ± 1.05 c | 148.29 ± 2.01 b | 194.21 ± 1.6 a |
Serine | 205.95 ± 3.01 c | 231.66 ± 2.03 b | 207.79 ± 2.2 c | 416.29 ± 3.1 a |
Arginine | ND a | ND a | ND a | ND a |
Citrulline | 1777.15 ± 19.1 d | 2205.1 ± 15.6 c | 2914.29 ± 14.7 a | 2454.09 ± 12.4 b |
β-Alanine | 42.89 ± 2.05 c | 55.34 ± 2.2 b | 76.81 ± 3.4 a | 53.47 ± 3.1 b |
γ-Amino-n-butyric acid | 174.81 ± 7.77 c | 620.44 ± 11.1 a | 599.41 ± 12.87 ab | 577.91 ± 18.01 b |
Ethanolamine | 44.65 ± 3.3 b | 65.05 ± 4.8 a | 63.81 ± 2.0 a | 45.4 ± 4.1 b |
Ammonia | 26.64 ± 1.2 bc | 28.7 ± 2.2 b | 24.52 ± 1.04 c | 33.01 ± 2.9 a |
1-Methylhistidine | 174.88 ± 3.1 d | 254.58 ± 1.4 c | 322.48 ± 3.2 b | 399.01 ± 3.02 a |
Total A. A | 2798.35 | 2617.31 | 2696.91 | 3773.05 |
Total essential A. A | 636.98 | 765.34 | 1085.4 | 1557.69 |
Total Metabolites | 2241.0005 | 3229.21 | 4001.33 | 3562.89 |
Average ± standard error from three separate replicates. Values with different lower-case letters in the same line are significantly different at p < 0.05 as determined by Duncan’s test. Total essential amino acids = sum of Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, and Valine. Total = sum of 17 amino acids. Total metabolites = sum of Citrulline, β-Alanine, γ-Amino-n-butyric acid, Ethanolamine, Ammonia, 1-methylhistidine.