Table 4.

Enantiomeric excess (e.e) and configuration of chiral terpenes extracted from three different cultivars based on three different extraction methods.

Volatile Compounds 1	Extraction Methods	Enantiomeric Excess(e.e, %) 2
		CP		CO		TO
		Confgn. 3	e.e	Confgn.	e.e	Confgn.	e.e
α-Pinene	SPME	+	86.96	+	90.31	+	71.55
	SD	+	87.89	+	93.91	+	66.91
	SE	+	87.79	+	94.25	+	67.78
Sabinene	SPME		nd 4	+/−	100.00	+/−	18.26
	SD		nd	+/−	100.00	+/−	41.51
	SE		nd	+/−	100.00	+/−	38.29
Limonene	SPME	+	49.57	+	91.52	+	24.09
	SD	+	56.70	+	96.74	+	23.29
	SE	+	58.54	+	97.48	+	29.89
β-Phellandrene	SPME	−	100.00	−	100.00	−	100.00
	SD	−	52.92	+	17.25	−	96.68
	SE	−	41.44	−	100.00	−	98.01
4-Terpineol	SPME	−	100.00	−	66.05	+	57.09
	SD	+	12.08	−	100.00	+	4.27
	SE	+	18.95		nd	+	6.65
Bornyl acetate	SPME	−	100.00	−	87.59	−	100.00
	SD	−	100.00	−	75.16	−	55.63
	SE	−	96.59	−	78.93	−	100.00
γ-Muurolene	SPME	+/−	47.84	+/−	100.00	+/−	72.85
	SD		nd	+/−	100.00	+/−	61.79
	SE	+/−	2.51	+/−	13.46	+/−	22.32
Caryophyllene	SPME	−	64.50	−	56.53	−	92.56
	SD	−	100.00	+	100.00	−	91.06
	SE	−	45.95	−	19.85	−	93.47
γ-Curcumene	SPME	+/−	52.10		nd	+/−	51.40
	SD	+/−	100.00		nd	+/−	100.00
	SE		nd		nd		nd

¹ The enantiomeric isomers separated on Cyclodex-B column; ² The enantiomer excess (e.e, %) = ((predominant enantiomer − minor enantiomer)/(predominant enantiomer + minor enantiomer)) × 100; ³ Confgn. = The optical configuration of predominant enantiomer as following criteria: +, (+)-isomers identified by authentic chiral standard or retention index; −, (−)-isomers identified by authentic chiral standard or retention index; +/−, were identified without distinction of chirality; ⁴ nd = not detected.