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. 2006 Nov 13;12(1):16–24. doi: 10.2478/s11658-006-0056-9

Prins and C-PRINS: Promising tools for the physical mapping of the lupin genome

Anna Kaczmarek 1, Barbara Naganowska 1, Bogdan Wolko 1
PMCID: PMC6275731  PMID: 17103086

Abstract

Two molecular cytogenetics methods, PRINS (primed in situ DNA labeling) and C-PRINS (cycling PRINS), were optimized for the physical mapping of several types of DNA sequences on the mitotic chromosomes of the narrow-leafed lupin (Lupinus angustifolius L.). The fragment of the FokI element from Vicia faba was localised by indirect PRINS reaction. Two other sequences, fragments of the coding sequences of L. luteus and of L. angustifolius, were localised by indirect C-PRINS. These techniques are faster and more sensitive than FISH, and they allowed the mapping of short DNA fragments. The data obtained shows that both types of PRINS are valuable tools for chromosome identification in lupin.

Key words: PRINS, C-PRINS, Physical mapping, Chromosomes, Lupinus angustifolius L

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Abbreviations used

BAC

bacterial artificial chromosome

C-PRINS

cycling PRINS

DAPI

4′,6′-diamidino-2-phenylindole

FISH

fluorescence in situ hybridization

PCR

polymerase chain reaction

PRINS

primed in situ DNA labeling

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