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. 2012 Apr 10;17(3):333–348. doi: 10.2478/s11658-012-0014-7

Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)

Ewa Olchowik 1, Karol Lotkowski 1, Saidmukhtar Mavlyanov 2, Nodira Abdullajanova 2, Maksim Ionov 3, Maria Bryszewska 3, Maria Zamaraeva 1,✉
PMCID: PMC6275782  PMID: 22491984

Abstract

Erythrocytes are constantly exposed to ROS due to their function in the organism. High tension of oxygen, presence of hemoglobin iron and high concentration of polyunsaturated fatty acids in membrane make erythrocytes especially susceptible to oxidative stress. A comparison of the antioxidant activities of polyphenol-rich plant extracts containing hydrolysable tannins from sumac leaves (Rhus typhina L.) and condensed tannins from grape seeds (Vitis vinifera L.) showed that at the 5–50 μg/ml concentration range they reduced to the same extent hemolysis and glutathione, lipid and hemoglobin oxidation induced by erythrocyte treatment with 400 μM ONOO− or 1 mM HClO. However, extract (condensed tannins) from grape seeds in comparison with extract (hydrolysable tannins) from sumac leaves stabilized erythrocytes in hypotonic NaCl solutions weakly. Our data indicate that both hydrolysable and condensed tannins significantly decrease the fluidity of the surface of erythrocyte membranes but the effect of hydrolysable ones was more profound. In conclusion, our results indicate that extracts from sumac leaves (hydrolysable tannins) and grape seeds (condensed tannins) are very effective protectors against oxidative damage in erythrocytes.

Key words: Tannins, Erythrocytes, Oxidative stress, Peroxynitrite, Hypochlorous acid, Hemolysis, Fluorescence anisotropy

Full Text

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Abbreviations used

DPH

1,6-diphenyl-1,3,5-hexatriene

GSH

reduced glutathione

HClO

hypochlorous acid

metHb

methemoglobin

ONOO−

peroxynitrite

TMA-DPH

1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene

Footnotes

Paper authored by participant of the international conference: 18th Meeting, European Association for Red Cell Research, Wrocław — Piechowice, Poland, May 12–15th, 2011. Publication cost was covered by the organizers of this meeting.

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