Abstract
The Arabidopsis AtGRP7 gene, encoding a glycine-rich RNA-binding protein, has been shown to be involved in the regulation of a circadian-regulated negative feedback loop. However, little is known about the role of AtGRP7 in mediating abscisic acid (ABA) and stress responses. Here, we show that AtGRP7 plays a role in both. AtGRP7 was repressed by ABA, high salt and mannitol. Disruption of AtGRP7 by T-DNA insertion led to hypersensitive responses to ABA in both seed germination and root growth assays. The atgrp7-1 mutant was also hypersensitive to osmotic stress conditions, such as high salt and high concentrations of mannitol. In addition, the atgrp7-1 mutant plants accumulated significantly higher transcript levels of two ABA-and stress-inducible genes, RD29A and RAB18, compared with the wild-type plants. Taken together, these results suggest that AtGRP7 is involved in the regulation of ABA and stress responses.
Key words: AtGRP7 gene, Abscisic acid, Osmotic stress
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Abbreviations used
- ABA
abscisic acid
- GR-RBP
glycine-rich RNA-binding protein
- MS
Murashige and Skoog
- TFs
transcription factors
References
- 1.Fedoroff N.V. Cross-talk in abscisic acid signaling. Sci. STKE. 2002;RE10:1–12. doi: 10.1126/stke.2002.140.re10. [DOI] [PubMed] [Google Scholar]
- 2.Finkelstein R.R., Gampala S.S.L., Rock C.D. Abscisic acid signaling in seeds and seedlings. Plant Cell (Suppl) 2002;14:S15–S45. doi: 10.1105/tpc.010441. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 3.Himmelbach A., Yang Y., Grill E. Relay and control of abscisic acid signaling. Curr. Opin. Plant Biol. 2003;6:470–479. doi: 10.1016/S1369-5266(03)00090-6. [DOI] [PubMed] [Google Scholar]
- 4.Giraudat J. Abscisic acid signaling. Curr. Opin. Cell Biol. 1995;7:232–238. doi: 10.1016/0955-0674(95)80033-6. [DOI] [PubMed] [Google Scholar]
- 5.Pandey G.K., Cheong Y.H., Kim K.N., Grant J.J., Li L., Hung W., D’Angelo C., Weinl S., Kudla J., Luan S. The calcium sensor calcineurin B-like 9 modulates abscisic acid sensitivity and biosynthesis in Arabidopsis. Plant Cell. 2004;16:1912–1924. doi: 10.1105/tpc.021311. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 6.Kim K.N., Cheong Y.H., Grant J.J., Pandey G.K., Luan S. CIPK3, a calcium sensor-associated protein kinase that regulates abscisic acid and cold signal transduction in Arabidopsis. Plant Cell. 2003;15:411–423. doi: 10.1105/tpc.006858. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7.Sridha S., Wu K. Identification of AtHD2C as a novel regulator of abscisic acid responses in Arabidopsis. Plant J. 2006;46:124–133. doi: 10.1111/j.1365-313X.2006.02678.x. [DOI] [PubMed] [Google Scholar]
- 8.Pandey G.K., Grant J.J., Cheong Y.H., Kim B.G., Li L., Luan S. ABR1, an APETALA2-domain transcription factor that functions as a repressor of ABA response in Arabidopsis. Plant Physiol. 2005;139:1185–1193. doi: 10.1104/pp.105.066324. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 9.Razem F.A., El-Kereamy A., Abrams S.R., Hill R.D. The RNA-binding protein FCA is an abscisic acid receptor. Nature. 2006;439:290–294. doi: 10.1038/nature04373. [DOI] [PubMed] [Google Scholar]
- 10.Sato N. A cold-regulated cyanobacterial gene cluster encodes RNAbinding protein and ribosomal protein S21. Plant Mol. Biol. 1994;24:819–823. doi: 10.1007/BF00029864. [DOI] [PubMed] [Google Scholar]
- 11.Sato N. A family of cold-regulated RNA-binding protein genes in the cyanobacterium Anabaena variabilis M3. Nucleic. Acids Res. 1995;23:2161–2167. doi: 10.1093/nar/23.12.2161. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 12.Gómez J., Sánchez M.D., Stiefel V., Rigau J., Puigdomènech P., Pagès M. A gene induced by the plant hormone abscisic acid in response to water stress encodes a glycine-rich protein. Nature. 1988;334:262–264. doi: 10.1038/334262a0. [DOI] [PubMed] [Google Scholar]
- 13.Bergeron D., Beauseigle D., Bellemare G. Sequence and expression of a gene encoding a protein with RNA-binding and glycine-rich domains in Brassica napus. Biochim. Biophys. Acta. 1993;1216:123–125. doi: 10.1016/0167-4781(93)90047-h. [DOI] [PubMed] [Google Scholar]
- 14.Hirose T., Sugita M., Sugiura M. cDNA structure, expression and nucleic acid-binding properties of three RNA-binding proteins in tobacco: occurrence of tissue-specific alternative splicing. Nucleic. Acids Res. 1993;21:3981–3987. doi: 10.1093/nar/21.17.3981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 15.Carpenter C.D., Kreps J.A., Simon A.E. Genes encoding glycinerich Arabidopsis thaliana proteins with RNA-binding motifs are influenced by cold treatment and an endogenous circadian rhythm. Plant Physiol. 1994;104:1015–1025. doi: 10.1104/pp.104.3.1015. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 16.Derry J.M., Kerns J.A., Francke U. RBM3, a novel human gene Xp11.23 with putative RNA-binding domain. Hum. Mol. Genet. 1995;4:2307–2311. doi: 10.1093/hmg/4.12.2307. [DOI] [PubMed] [Google Scholar]
- 17.Kwak K.J., Kim Y.O., Kang H. Characterization of transgenic Arabidopsis plants overexpressing GR-RBP4 under high salinity, dehydration, or cold stress. J. Exp. Bot. 2005;56:3007–3016. doi: 10.1093/jxb/eri298. [DOI] [PubMed] [Google Scholar]
- 18.Heintzen C., Nater M., Apel K., Staiger D. AtGRP7, a nuclear RNA-binding protein as a component of a circadian-regulated negative feedback loop in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA. 1997;94:8515–8520. doi: 10.1073/pnas.94.16.8515. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 19.Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 1962;15:473–497. doi: 10.1111/j.1399-3054.1962.tb08052.x. [DOI] [Google Scholar]
- 20.Alonso J.M., Stepanova A.N., Leisse T.J., Kim C.J., Chen H., Shinn P., Stevenson D.K., Zimmerman J., Barajas P., Cheuk R., Gadrinab C., Heller C., Jeske A., Koesema E., Meyers C.C., Parker H., Prednis L., Ansari Y., Choy N., Deen H., Geralt M., Hazari N., Hom E., Karnes M., Mulholland C., Ndubaku R., Schmidt I., Guzman P., Aguilar-Henonin L., Schmid M., Weigel D., Carter D.E., Marchand T., Risseeuw E., Brogden D., Zeko A., Crosby W.L., Berry C.C., Ecker J.R. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science. 2003;301:653–657. doi: 10.1126/science.1086391. [DOI] [PubMed] [Google Scholar]
- 21.Staiger D., Apel K. Circadian clock-regulated expression of an RNA-binding protein in Arabidopsis: characterisation of a minimal promoter element. Mol. Gen. Genet. 1999;261:811–819. doi: 10.1007/s004380050025. [DOI] [PubMed] [Google Scholar]
- 22.Zimmermann P., Hirsch-Hoffmann M., Hennig L., Gruissem W. GENEVESTIGATOR. Arabidopsis microarray database and analysis toolbox. Plant Physiol. 2004;136:2621–2632. doi: 10.1104/pp.104.046367. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 23.Rock C.D. Pathways to abscisic acid-regulated gene expression. New Phytol. 2000;148:357–396. doi: 10.1046/j.1469-8137.2000.00769.x. [DOI] [PubMed] [Google Scholar]
- 24.Leung J., Giraudat J. Abscisic acid signal transduction. Annu. Rev. Plant Physiol. Plant Mol. Biol. 1998;49:199–222. doi: 10.1146/annurev.arplant.49.1.199. [DOI] [PubMed] [Google Scholar]
- 25.Nishiyama H., Itoh K., Kaneko Y., Kishishita M., Yoshida O., Fujita J. A glycine-rich RNA-binding protein mediating cold-inducible suppression of mammalian cell growth. J. Cell Biol. 1997;137:899–908. doi: 10.1083/jcb.137.4.899. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 26.Seo M., Koshiba T. Complex regulation of ABA biosynthesis in plants. Trends Plant Sci. 2002;7:41–48. doi: 10.1016/S1360-1385(01)02187-2. [DOI] [PubMed] [Google Scholar]
- 27.Zeevaart J.A.D., Creelman R.A. Metabolism and physiology of abscisic acid. Annu. Rev. Plant Physiol. Plant Mol. Biol. 1988;39:439–473. doi: 10.1146/annurev.pp.39.060188.002255. [DOI] [Google Scholar]