Abstract
The complete nucleotide sequence of the cucumber (C. sativus L. var. Borszczagowski) chloroplast genome has been determined. The genome is composed of 155,293 bp containing a pair of inverted repeats of 25,191 bp, which are separated by two single-copy regions, a small 18,222-bp one and a large 86,688-bp one. The chloroplast genome of cucumber contains 130 known genes, including 89 protein-coding genes, 8 ribosomal RNA genes (4 rRNA species), and 37 tRNA genes (30 tRNA species), with 18 of them located in the inverted repeat region. Of these genes, 16 contain one intron, and two genes and one ycf contain 2 introns. Twenty-one small inversions that form stem-loop structures, ranging from 18 to 49 bp, have been identified. Eight of them show similarity to those of other species, while eight seem to be cucumber specific. Detailed comparisons of ycf2 and ycf15, and the overall structure to other chloroplast genomes were performed.
Key words: Organelle, Gene order
Full Text
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Abbreviations used
- cpDNA
chloroplast DNA
- IR
inverted repeat
- JLA
junction IRA/LSC
- JLB
junction IRB/LSC
- JSA
junction IRA/SSC
- JSB
junction IRB/SSC
- LSC
large single copy
- PCR
polymerase chain reaction
- rRNA
ribosomal RNA
- SSC
small single copy
- tRNA
transport RNA
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