Abstract
This study focused on the function of hnRNP-R in the regulation of c-fos expression. We demonstrated that hnRNP-R accelerated the rise and decline phases of c-fos mRNAs and Fos proteins, allowing PMA to induce an augmented pulse response of c-fos expression. Then, we examined the role of the c-fos-derived AU-rich element (ARE) in hnRNP-R-regulated mRNA degradation. Studies with the ARE-GFP reporter gene showed that hnRNP-R significantly reduced the expression of GFP with an inserted ARE. Moreover, immunoprecipitation-RT-PCR analysis demonstrated that in R28 cells and rat retinal tissues, the c-fos mRNA was co-immunoprecipitated with hnRNP-R. These findings indicate that hnRNP-R regulates the c-fos expression in retinal cells, and that the ARE of c-fos mRNAs contributes to this regulation.
Keywords: hnRNP-R, Retina, c-fos, mRNA turnover, ARE
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Abbreviations
- ARE
AU-rich element
- DMSO
dimethyl sulfoxide
- GFP
green fluorescence protein
- PMA
phorbol 12-myristate 13-acetate
- SMA
spinal muscular atrophy
- UTR
3′-untranslated region
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