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. 2011 Dec 28;17(1):153–170. doi: 10.2478/s11658-011-0039-3

Developmental expression of P5 ATPase mRNA in the mouse

Lisa S Weingarten 1, Hardi Dave 2, Hongyan Li 2, Dorota A Crawford 1,2,3,✉
PMCID: PMC6275865  PMID: 22207337

Abstract

P5 ATPases (ATP13A1 through ATP13A5) are found in all eukaryotes. They are currently poorly characterized and have unknown substrate specificity. Recent evidence has linked two P5 ATPases to diseases of the nervous system, suggesting possible importance of these proteins within the nervous system. In this study we determined the relative expression of mouse P5 ATPases in development using quantitative real time PCR. We have shown that ATP13A1 and ATP13A2 were both expressed similarly during development, with the highest expression levels at the peak of neurogenesis. ATP13A3 was expressed highly during organogenesis with one of its isoforms playing a more predominant role during the period of neuronal development. ATP13A5 was expressed most highly in the adult mouse brain. We also assessed the expression of these genes in various regions of the adult mouse brain. ATP13A1 to ATP13A4 were expressed differentially in the cerebral cortex, hippocampus, brainstem and cerebellum while levels of ATP13A5 were fairly constant between these brain regions. Moreover, we demonstrated expression of the ATP13A4 protein in the corresponding brain regions using immunohistochemistry. In summary, this study furthers our knowledge of P5-type ATPases and their potentially important role in the nervous system.

Key words: P5-type ATPases, mRNA expression, Neurogenesis, Parkinson’s disease, Autism spectrum disorders, Real-time PCR, Immunohistochemistry

Full Text

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Abbreviations used

ASD

autism spectrum disorders

B2m

beta-2-microglobulin

DAB

3,3′-diaminobenzidine

DTT

dithiothreitol

Gapdh

glyceraldehyde 3-phosphate dehydrogenase

Gusb

beta-glucuronidase

HPRT

hypoxanthine phosphoribosyl transferase

NGS

normal goat serum

PBS

phosphate buffered saline

PBS-T

phosphate buffered saline Tween 20

PCR

polymerase chain reaction

PD

Parkinson’s disease

PFA

paraformaldehyde

Pgk1

phosphoglycerate kinase 1

qPCR

quantitative polymerase chain reaction

Tfrc

transferring receptor

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