Abstract
Preservation of hepatocyte functions in vitro will undoubtedly help the management of acute liver failure. The coculture system may be able to prevent functional decline of hepatocytes. It has already been shown that hepatocytes, when cocultured with bone marrow mesenchymal stem cells, could undergo long-term culture in vitro without loss of functions. In this study, human orbital fat-derived stem cells were isolated and cocultured with rat hepatocytes. When treated with serum from an acute liver failure patient, rat hepatocyte monoculture showed reduction of cell viability and loss of liverspecific functions. However, rat hepatocytes in the coculture system were still able to secret albumin and synthesize urea. IL-6 was significantly elevated in the coculture of rat hepatocyte with orbital fat-derived stem cells, and it might be the key immunoregulator which protects rat hepatocytes against inflammation. Our data confirmed that orbital fat-derived stem cells, or other adipose tissue-derived stem cells, are an ideal candidate to support rat hepatocyte functions in vitro.
Key words: Acute liver failure, Coculture, Differentiation, Fat tissue, Hepatocytes, Inflammation, Interleukin-6, Mesenchymal stem cells, Orbital fat-derived stem cells, Serum
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Abbreviations used
- ALF
acute liver failure
- ADSCs
adipose tissue-derived stem cells
- ALB
albumin
- CK32
connexin 32
- CYP3A4
cytochrome P450 subtype 3A4
- IDO
indoleamine 2,3-dioxygenase
- IFN
interferon
- IL
interleukin
- GAPDH
glyceraldehyde-3-phosphate dehydrogenase
- MSCs
mesenchymal stem cells
- OFSCs
orbital fat-derived stem cells
- PAS
periodic acid-Schiff
- TDO2
tryptophan 2,3-dioxygenase
- TNF
tumor necrotic factor
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