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. 2010 Aug 28;15(4):600–610. doi: 10.2478/s11658-010-0031-3

TRPC expression in mesenchymal stem cells

Frederic Torossian 1,2, Aurelie Bisson 1, Jean-Pierre Vannier 2, Olivier Boyer 1, Marek Lamacz 1,3,✉
PMCID: PMC6275936  PMID: 20803258

Abstract

Transient receptor potential canonical (TRPC) channels are key players in calcium homeostasis and various regulatory processes in cell biology. Little is currently known about the TRPC subfamily members in mesenchymal stem cells (MSC), where they could play a role in cell proliferation. We report on the presence of TRPC1, 2, 4 and 6 mRNAs in MSC. Western blot and immunofluorescence staining indicate a membrane and intracellular distribution of TRPC1. Furthermore, the decrease in the level of TRPC1 protein caused by RNA interference is accompanied by the downregulation of cell proliferation. These results indicate that MSC express TRPC1, 2, 4 and 6 mRNA and that TRPC1 may play a role in stem cell proliferation.

Key words: Mesenchymal stem cells, TRPC1, Cell proliferation

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Abbreviations used

MSC

Mesenchymal stem cells

TRP

Transient receptor potential

TRPC

Transient receptor potential canonical

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