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. 2006 Dec 28;12(2):240–252. doi: 10.2478/s11658-006-0069-4

The molecular diversity of different isolates of Beauveria bassiana (Bals.) Vuill. as assessed using intermicrosatellites (ISSRs)

M Elena Estrada 1,2, Manuel V Camacho 1, César Benito 1,✉
PMCID: PMC6275939  PMID: 17192823

Abstract

Inter-microsatellite PCR (ISSR-PCR) markers were used to identify and to examine the genetic diversity of eleven Beauveria bassiana isolates with different geographic origins. The variability and the phylogenetic relationships between the eleven strains were analyzed using 172 ISSR-PCR markers. A high level of polymorphism (near 80%) was found using these molecular markers. Seven different isolates showed exclusive bands, and ISSR primer 873 was able to distinguish between all the strains. The dendrogram obtained with these markers is robust and in agreement with the geographical origins of the strains. All the isolates from the Caribbean region were grouped together in a cluster, while the other isolates grouped in the other cluster. The similarity exhibited between the two clusters was less than 50%. This value of homology shows the high genetic variability detected between the isolates from the Caribbean region and the other isolates. ISSR-PCR markers provide a quick, reliable and highly informative system for DNA fingerprinting, and allowed the identification of the different B. bassiana isolates studied.

Key words: Beauveria bassiana, Strain identification, Inter-microsatellite, Phylogenetic relationships

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Abbreviations used

AFLP

amplified fragment length polymorphism

EST

expressed sequence tag

ISSR

inter simple sequence repeat

ITS

internal transcribed spacer

PCR

polymerase chain reaction

RAPD

random amplified polymorphic DNA

RFLP

restriction fragment length polymorphism

Rp

resolving power

SSCP

single-strand conformation polymorphism

SSR

simple sequence repeat

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