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Cellular & Molecular Biology Letters logoLink to Cellular & Molecular Biology Letters
. 2007 Mar 15;12(3):448–456. doi: 10.2478/s11658-007-0017-y

The effect of growth medium on the antioxidant defense of Saccharomyces cerevisiae

Ewa Macierzyńska 1,✉, Agnieszka Grzelak 1, Grzegorz Bartosz 1,2
PMCID: PMC6275951  PMID: 17361365

Abstract

We compared the oxidation of dihydrorhodamine 123, glutathione contents and activities of superoxide dismutase (SOD) and catalase for three wild-type strains of Saccharomyces cerevisiae grown on media with different carbon sources. The rate of oxidation of dihydrorhodamine 123 was much higher in respiring cells grown on ethanol or glycerol media than in fermenting cells grown on glucose medium. The total SOD activity was highest on glycerol medium and lowest on ethanol medium, while the catalase activity was highest on glycerol medium. The sequence of glutathione content values was: glucose > ethanol > glycerol.

Key words: Yeast, Saccharomyces cerevisiae, Reactive oxygen species, Superoxide dismutase, Catalase, Glutathione

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Abbreviations used

DTNB

5,5′-dithiobis-(2-nitrobenzoic acid)

H2R 123

dihydrorhodamine 123

MES

2-morpholinoethanesulfonic acid

ROS

reactive oxygen species

SOD

superoxide dismutase

YPD medium

1% yeast extract, 1% Bacto-peptone, 2% glucose, YPG medium, 1% yeast extract, 1% Bacto-peptone,2% glycerol

YPE medium

1% yeast extract, 1% Bacto-peptone, 3% ethanol

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