Abstract
Heterogeneous human amniotic fluid contains various cell types. Herein, we report on the possibility of simultaneously isolating three subtypes of cells from one primary culture. Using a stainless steel instrument named a colony poculum, two of the three cell subtypes could be efficiently cultured, and these were further characterized. The results indicated that these two cell subtypes had different morphologies and were characterized by different cell marker expression profiles, including the differential expression of CD105, CD117 and EBAF. Furthermore, their gene expression array data revealed their different gene expression profiles. Although both cell types expressed several embryonic stem cell-specific markers, they were non-tumorigenic in vivo. This paper not only provides new insight into the heterogeneity of human amniotic fluid, it also presents a simple yet efficient cell isolation method. These results will contribute to the thorough investigation of the properties and potential future applications of human amniotic fluid-derived cells.
Electronic Supplementary Material
Supplementary material is available for this article at 10.2478/s11658-010-0017-1 and is accessible for authorized users.
Electronic Supplementary Material
Supplementary material is available for this article at 10.2478/s11658-010-0017-1 and is accessible for authorized users.
Key words: Human amniotic fluid, Cell subtypes, Isolation, Colony poculum
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Abbreviations used
- AF-type
amniotic fluid-specific
- DAPI
dipeptidyl aminopeptidase
- EC cells
embryonal carcinoma cells
- EG cells
embryonic germ cells
- ES cells
embryonic stem cells
- E-type
epitheloid type
- FITC
fluorescein isothiocyanate
- F-type
fibroblastic type
- GO
gene ontology
- hAFC
human amniotic fluid-derived cells
- PBS
phosphate-buffered saline
- PE
phycoerythrin
- RT
room temperature
- RT-PCR
reverse transcriptase polymerase chain reaction
Contributor Information
Junmei Zhou, Phone: 86-21-62470059, FAX: 86-21-62790494, Email: jemyzh@vip.sina.com.
Fang Chen, Email: cdbxh@yahoo.cn.
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Supplementary Materials
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Supplementary material, approximately 16.6 MB.