Abstract
The erythrocytes of 12-month old Sod1 −/− mice showed an increased level of reactive oxygen species (ROS), as estimated by the degree of dihydroethidine and dihydrorhodamine oxidation, and the increased level of Heinz bodies. No indices of severe oxidative stress were found in the red blood cells and blood plasma of Sod1 −/− mice as judged from the lack of significant changes in the levels of erythrocyte and plasma glutathione, plasma protein thiol and carbonyl groups and thiobarbituric-acid reactive substances in the blood plasma. However, a decreased erythrocyte lifespan, increased reticulocyte count and splenomegaly were noted, indicating the importance of superoxide dismutase for maintaining erythrocyte viability. The levels of erythrocyte ROS and Heinz bodies and the reticulocyte count were indistinguishable in Sod1 +/+ and Sod1 +/− mice, suggesting that a superoxide dismutase activity decrease to half of its normal value may be sufficient to secure the protective effects of the enzyme.
Key words: Superoxide dismutase, Erythrocyte, Red blood cell, Reactive oxygen species, Oxidative stress, Heinz bodies, Acetylcholinesterase
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Abbreviations used
- DMSO
dimethylsulfoxide
- DT
dithionite
- DTPA
diethylenetriaminepentaacetatic acid
- EDTA
ethylenediaminetetraacetic acid
- GPx
glutathione peroxidase
- GSH
glutathione
- GSSG
glutathione disulfide
- Hb
hemoglobin
- HB
Heinz bodies
- HBS
Hank’s buffered solution
- Ig
immunoglobulin
- KO
knockout
- NEM
N-ethylmaleimide
- NHS
N-hydroxysuccinimide
- PE
phycoerythrin
- Prdx
peroxiredoxin
- RBC
red blood cell
- SOD
superoxide dismutase
- TBARS
thiobarbituric-acid reactive substances
- tBOOH
tert-butyl hydroperoxide
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