Abstract
MicroRNAs (miRNAs) regulate gene expression by inhibiting translation or targeting messenger RNA (mRNA) for degradation in a posttranscriptional fashion. In this study, we show that ectopic expression of miR-34a-5p reduces the mRNA and protein levels of Krüppel-like factor 4 (KLF4). We also demonstrate that miR-34a targets the 3′-untranslated mRNA region of KLF4 and show that overexpression of miR-34a induces a significant level of apoptosis in BNL CL.2 cells exposed to doxorubicin or 10 Gy X-ray. Our data suggest that the effects of miR-34a on apoptosis occur due to the downregulation of KLF4.
Key words: MiR-34a, KLF4, Apoptosis, Liver, Irradiation, Doxorubicin
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Abbreviations used
- ALDH2
aldehyde dehydrogenase-2
- BCL2
B-cell lymphoma-2
- FCM
flow cytometry
- GAPDH
glyceraldehyde-3-phosphate dehydrogenase
- HEK
human embryonic kidney
- KLF4
Krüppel-like factor 4
- TBST
Tris-buffered saline with Tween
- UTR
untranslated region
Footnotes
Qiu Chen and Lei Li contributed equally to this work
Contributor Information
Feng Mei Cui, Email: cuifengmei@suda.edu.cn.
Jun Hou, Email: hou.jun@zs-hospital.sh.cn.
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