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. 2011 Jul 18;16(3):431–451. doi: 10.2478/s11658-011-0016-x

Quantitative and dynamic expression profile of premature and active forms of the regional ADAM proteins during chicken brain development

Annett Markus 1, Xin Yan 1, Arndt Rolfs 1, Jiankai Luo 1,✉
PMCID: PMC6276021  PMID: 21786032

Abstract

The ADAM (A Disintegrin and Metalloprotease) family of transmembrane proteins plays important roles in embryogenesis and tissue formation based on their multiple functional domains. In the present study, for the first time, the expression patterns of the premature and the active forms of six members of the ADAM proteins — ADAM9, ADAM10, ADAM12, ADAM17, ADAM22 and ADAM23 — in distinct parts of the developing chicken brain were investigated by quantitative Western blot analysis from embryonic incubation day (E) 10 to E20. The results show that the premature and the active forms of various ADAM proteins are spatiotemporally regulated in different parts of the brain during development, suggesting that the ADAMs play a very important role during embryonic development.

Key words: ADAM, Gene expression, Protein, Brain development, Chicken

Full Text

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Abbreviations used

ADAM

A Disintegrin and Metalloprotease

APP

amyloid precursor protein

BCA

bicinchoninic acid

CNS

central nervous system

ECM

extracellular matrix

EGF

epidermal growth factor

EGFR

epidermal growth factor receptor

ELISA

enzyme-linked immunosorbent assay

HE-EGF

heparin-binding EGFlike growth factor

kDa

kilodalton

GAPDH

glyceraldehyde-3-phosphate dehydrogenase

LGI1

leucine-rich glioma inactivated 1

RT-PCR

reverse transcriptionpolymerase chain reaction

SDS

sodium dodecyl sulfate

TACE

tumor necrosis factor alpha converting enzyme

TBST

tris-buffered saline-Tween

TGF-α

transforming growth factor-α

TNF-α

tumor necrosis factor-α

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