Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Genetic reagent (Drosophila melanogaster)	esgGal4	Kyoto DGGR	DGRC:112304; FLYB:FBti0033872;	FlyBase symbol: w[*]; P{w[+mW.hs]=GawB}NP0726/CyO
Genetic reagent (D. melanogaster)	ubi-his2av::mRFP	Bloomington Drosophila Stock Center	BDSC:23650; FLYB:FBti0077846; RRID:BDSC_23650	FlyBase symbol: w[*]; P{w[+mC]=His2Av-mRFP1}III.1
Genetic reagent (D. melanogaster)	breathlessGal4, UAS-cyt-GFP	Other		w; btl-Gal4, UAS-cytGFP shared by Mark Metzstein
Genetic reagent (D. melanogaster)	UAS-LifeactGFP	Bloomington Drosophila Stock Center	BDSC:35544; FLYB:FBti0143326; RRID:BDSC_35544	FlyBase symbol: y[1] w[*]; P{y[+t*] w[+mC]=UAS-Lifeact-GFP}VIE-260B
Genetic reagent (D. melanogaster)	UAS-his2b::CFP	PMID: 24850412		w; UAS-his2b::CFP/ (Cyo); + -- shared by Yoshihiro Inoue
Genetic reagent (D. melanogaster)	GBE-Su(H)-GFP:nls	PMID: 22522699		w?; mw, GBE-Su(H)-GFPnls/(Cyo); Dr/TM6B -- from (de Navascués et al., 2012) shared by Joaquin de Navascues
Genetic reagent (D. melanogaster)	act5c-spaghetti squash::GFP	PMID:12105185		w?; act5c-sqh::GFP; Dr/TM6C -- shared by Denise Montell
Genetic reagent (D. melanogaster)	ubi-E-cadherin::YFP	PMID: 24855950		w; ubi-E-cadherin::YFP; + -- shared by Denise Montell
Chemical compound, drug	Concanavalin-A-Alexa647	Invitrogen	Invitrogen:C21421	25 μg/ml final concentration
Chemical compound, drug	Sytox Green	ThermoFisher	ThermoFisher:S7020	1 μM final concentration
Chemical compound, drug	SiR-tubulin	Cytoskeleton	Cytoskeleton:CY-SC002	0.5 μM final concentration
Chemical compound, drug	Human insulin	Sigma Aldrich	Sigma-Aldrich:I0516	100 μg/ml final concentration
Software, algorithm	Fiji	Other	RRID:SCR_002285	StackRegfrom Arganda-Arganda-Carreras et al., 2006b; Correct 3D drift from Parslow et al. (2014); Bioformats plugin
Software,  algorithm	Bitplane Imaris	Other	RRID:SCR_007370	Surpass module; Surface Recognition Wizard; Measurement Points tool