Fig. 2.

LncRNA PVT1 functioned as a sponge of miR-128-3p. (A) The predicted wild-type or mutated miR-128-3p binding sites in PVT1. (B, C) RIP assay with antibody Ago2, IgG, or 10% input from U87 and U251 cell extracts. RNA levels of PVT1 and miR-128-3p in immunoprecipitates were examined by qRT-PCR. ^**p < 0.01. (D) Luciferase reporter assay was performed to detect luciferase activity in U87 and U251 cells co-transfected with the constructed luciferase reporter plasmids (PVT1-WT or PVT1-MUT) and miR-128-3p or miR-control. ^**p < 0.01, ^##p < 0.01