Figure 3.
Effects of single component IMG and ES isolated from natural product mixture (RPH) on the expression of OA-related molecules in IL-1β-stimulated SW1353 cells. SW1353 cells (1 × 106 cells) were stimulated with 20 μg/mL IL-1β for 24 h. IMG (isomucronulatol 7-β-O-glucoside, 30, 50 or 100 μg/mL), ES (ecliptasaponin A, 10, 30 or 50 ng/mL), or combination (each dose of IMG + 50 ng/mL ES) was pretreated at 1 h before IL-1β stimulation. The expression of OA-related molecules including collagen type II was determined in mRNA extracts isolated the cell lysates using RT-PCR, and Western blot, respectively. (Upper panel) The mRNA expression of OA-related molecules (MMP13, TNF-α, IL-1β, COX1/2). (Lower panel) The protein expression of OA-related molecules (MMP13, Collagen type II, TNF-α, IL-1β, and COX-2). #, Numbers below band images are the mean values (n = 4) obtained from as described in the Figure 1 legend. NC, negative control; IL-1β (interleukin 1 beta), IL-1β-stimulated SW1353 cells; DMSO (0.3%), control for the solvent of a single component; Coll-II, collagen type II.