Table 1.
Binding selectivity assays for CAF and THP MIPs and NIPs.
| Polymers | Template | Single Analyte Binding | Competitive Binding | ||||
|---|---|---|---|---|---|---|---|
| CAF (µmol) | THP (µmol) | CAF | THP | ||||
| µmol | fB 1 | µmol | fB 1 | ||||
| IM1000 | CAF | 3.61 ± 0.10 | - | 1.93 ± 0.07 | 0.63 | 1.12 ± 0.13 | 0.37 |
| THP | - | 4.59 ± 0.21 | 0.97 ± 0.02 | 0.28 | 2.52 ± 0.20 | 0.72 | |
| NIP | 3.32 ± 0.10 | 3.86 ± 0.28 | 1.50 ± 0.03 | 0.52 | 1.36 ± 0.04 | 0.48 | |
|
IM100 2
MX5 TM4 |
CAF | 4.70 ± 0.20 | - | 3.76 ± 0.01 | 0.77 | 1.15 ± 0.07 | 0.23 |
| THP | - | 5.40 ± 0.20 | 0.79 ± 0.12 | 0.13 | 5.92 ± 0.10 | 0.88 | |
| NIP | 3.59 ± 0.15 | 3.55 ± 0.29 | 1.33 ± 0.15 | 0.58 | 0.96 ± 0.15 | 0.42 | |
| IM5 | CAF | 4.91 ± 0.24 | - | 4.52 ± 0.25 | 0.84 | 0.87 ± 0.04 | 0.16 |
| THP | - | 5.97 ± 0.36 | 0.40 ± 0.01 | 0.07 | 5.38 ± 0.02 | 0.93 | |
| NIP | 2.82 ± 0.12 | 3.07 ± 0.29 | 1.38 ± 0.03 | 0.51 | 1.32 ± 0.01 | 0.49 | |
| MX2 | CAF | 14.10 ± 0.33 | - | 9.64 ± 0.05 | 0.69 | 4.31 ± 0.01 | 0.31 |
| THP | - | 14.7 ± 1.10 | 1.36 ± 0.01 | 0.11 | 11.83 ± 0.61 | 0.89 | |
| NIP | 12.25 ± 0.07 | 11.97 ± 1.21 | 5.28 ± 0.05 | 0.47 | 6.01 ± 0.02 | 0.53 | |
| MX10 | CAF | 3.80 ± 0.01 | - | 2.28 ± 0.06 | 0.73 | 0.85 ± 0.01 | 0.27 |
| THP | - | 4.00 ± 0.20 | 0.23 ± 0.01 | 0.06 | 3.75 ± 0.04 | 0.94 | |
| NIP | 2.36 ± 0.01 | 2.12 ± 0.15 | 1.40 ± 0.13 | 0.59 | 0.98 ± 0.11 | 0.41 | |
1 Binding with respect to total CAF + THP binding. 2 This polymer has been used for the I:tM, fM:X and T:fM studies and thus has been assigned 3 different codes.