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. 2018 Oct 27;23(11):2785. doi: 10.3390/molecules23112785

Figure 3.

Figure 3

Dynamic of permeation of SYTO 9 into S. aureus 209 P (a) and E. coli MG 1655 (b) cells treated with emericellipsin A. Designations: 1—75 μg/mL; 2—32.3 μg/mL; 3—16 μg/mL; 4—negative control; 5—positive control. If bacterial membranes are permeabilized, PI penetrates into the cell. What follows is SYTO 9 getting displaced from DNA, which leads to a decrease in luminescence intensity in a green region of the spectrum. Pure-water and 20% alcohol served as negative and positive controls, respectively. Arrows show the time of test-substance.