Figure 4.

The effects of compounds 1–7 on the differentiation of MSCs toward osteoblasts or adipocytes. The mouse mesenchymal stem cell line, C3H10T1/2, was treated with compounds 1–7. After osteogenic differentiation, the cells were stained with ALP (A) and ALP enzyme activity was measured (B). In the separate plates, the cells were differentiated into adipocytes prior to ORO staining (C). Stained cells were quantitatively evaluated by resolving stained lipid droplets and measuring absorbance at the red wavelength (D). Ctrl represents untreated negative control. 5 μM of oryzativol A (OryA) was added to the experimental set as a positive osteogenesis control. 20 μM of resveratrol (Res) was used as a positive control in adipogenesis. 10 μM of each of the compounds was added to the osteogenesis- or adipogenesis-differentiation medium. * denotes p < 0.05 and *** denotes p < 0.001.