Table 3. Immunofluorescence protocols for primary antibodies.
Secondary
Labeling Antibody |
Manufacturer | Raised in | React with | Recommended
dilution range |
Fluorescence | Targeted primary antibody
(cells) |
---|---|---|---|---|---|---|
Alexa Fluor® 488 | Invitrogen (A-11008)
Carisbad, CA, USA |
Goat | Rabbit | 1:200 – 1:2000 | Green | TLR2 |
Alexa Fluor® 594 | Invitrogen (A-21203)
Carisbad, CA, USA |
Donkey | Mouse | 1:200 – 1:2000 | Red | CD38 (lymphocyte, plasma cell)
CD68 (macrophage) Cd83 (matured dendritic cell) |