Figure 2. Nephron numbers are reduced in Wnt11^tm1a/tm1a adults leading to compensatory hypertrophy.

(A) The physical disector/fractionator method was used to estimate glomerular number in 6 week old animals. A significant reduction is found in Wnt11 mutants. (B) Renal corpuscle volume is increased in Wnt11 mutant kidneys. (C) Glomerular volume is larger in Wnt11^tm1a/tm1a kidneys. (D) Estimated kidney volume is reduced in Wnt11 mutants. (E) Histological sections stained with hematoxylin and eosin highlight that overall morphology is preserved, although glomerular/corpuscle volume is increased in the Wnt11 mutants. All error bars represent SEM. All significance values were determined by t-test. ns = p > 0.05, * = p < 0.05, ** = p < 0.01, *** = p < 0.001. n = 6 for each genotype.