New trends and applications in carboxylation for isotope chemistry

Abstract

Carboxylations are an important method for the incorporation of isotopically labeled ¹⁴CO₂ into molecules. This manuscript will review labeled carboxylations since 2010 and will present a perspective on the potential of recent unlabeled methodology for labeled carboxylations. The perspective portion of the manuscript is broken into 3 major sections based on product type, arylcarboxylic acids, benzylcarboxylic acids, and alkyl carboxylic acids, and each of those sections is further subdivided by substrate.

1. INTRODUCTION

The common precursor for the synthesis of all ¹⁴C‐labeled compounds is Ba¹⁴CO₃. Ba¹⁴CO₃ can be converted to numerous useful starting materials including ¹⁴C cyanides and ¹⁴C acetylene, but arguably, the most used of the ¹⁴C reagents is ¹⁴CO₂. It can be reduced to afford ¹⁴CO, H¹⁴CO₂H, H¹⁴CHO, or ¹⁴CH₃OH, and from these, many other 1‐carbon synthons can be prepared including ¹⁴CH₃I, ¹⁴COCl₂, and ¹⁴CH₃NO₂. ¹⁴CO₂ can also be used directly to give ¹⁴C‐labeled carboxylic acids such as ¹⁴C‐labeled acetate or benzoic acids. This perspective will focus on recent ¹⁴C carboxylations and modern methodology that could be applied to ¹⁴C carboxylations. Carboxylic acids can also be formed in 1 step via carbonylation, but that will not be considered in this review. Neither will multiple step processes such as cyanation followed by hydrolysis or formylation followed by oxidation. This topic has not been reviewed previously, but a monograph on the synthesis of tritium and ¹⁴C‐labeled compounds does cover the traditional carboxylation techniques.1 In addition, there are several literature reviews covering carboxylations which are of potential use in radiochemical applications.2, 3, 4 While this manuscript focuses on the use of ¹⁴CO₂, the methods described could also be applied to ¹³CO₂. The focus on ¹⁴CO₂ is intentional as the need to adhere to the use of stoichiometric or near stoichiometric amounts of CO₂ is more critical for C‐14 than for C‐13 because of the cost of the reagent and the radiochemical waste produced.

2. HANDLING OF ¹⁴CO₂

Ba¹⁴CO₃ can be converted to ¹⁴CO₂ by treatment with H₂SO₄ 5 or by heating with PbCl₂.6 The later method leads to heavy metal radioactive waste, which is best avoided. Once liberated, ¹⁴CO₂ can easily be manipulated because it is highly volatile (BP −78°C) but has a low vapor pressure when cooled in liquid nitrogen (−196°C).7 In 2001, Bannwart and coworkers reported a ¹⁴CO₂ manifold system which greatly facilitates the handling of ¹⁴CO₂.8 The manifold uses ¹⁴CO₂ absorbed on molecular sieves which is stored at room temperature. The ¹⁴CO₂ can be liberated by heating the sieves, and the excess ¹⁴CO₂ can be retrapped onto the molecular sieves. The molecular sieve reservoir is attached to a stainless steel manifold which can be evacuated to very low pressures. The ¹⁴CO₂ released into the manifold can be accurately measured and quantified and the specified amount easily transferred into a reaction flask. This avoids the need to generate ¹⁴CO₂ each time a reaction is run. The manifold greatly improved the speed and efficiency of ¹⁴C carboxylation reactions and greatly facilitates reaction optimization. At the same time, it reduces the waste generated by the reaction as the radioactive sulfuric acid waste is only generated when loading the manifold with ¹⁴CO₂ (if ¹⁴CO₂ is not used directly).

3. RECENT CARBOXYLATIONS USING ¹⁴CO₂

Carboxylation using ¹⁴CO₂ has long been known,5, 9 and it remains a frequently used methodology for the incorporation of ¹⁴C into molecules.1 This is in part because of the robust nature of the reaction, the simplicity of the reaction design, and the relatively low cost of ¹⁴CO₂ compared to other ¹⁴C starting materials. Traditional carboxylation reactions—coupling of organolithium or Grignard reagents with CO₂—require harsh reaction conditions thereby requiring early installation of the ¹⁴C because of functional group incompatibility. For example, Seidel and Pleiss recently reported the synthesis of [¹⁴C]cinaciguat in which bromide 1 was lithiated and the organolithium carboxylated with ¹⁴CO₂ (Scheme 1).10 The resulting acid was converted to the target compound in 4 steps.

Scheme 1.

The carboxylation of an aryllithium reagent en route to [¹⁴C]cinaciguat10

The relative low cost of ¹⁴CO₂ allows for earlier introduction of the ¹⁴C label than would otherwise be considered. For example, in a recent report of the synthesis of [¹⁴C]AZD4694, a synthesis with 10 radioactive steps and 5% overall yield is reported (Scheme 2).11 Such a lengthy synthesis would be much less likely to be pursued with an expensive starting ¹⁴C source.

Scheme 2.

The carboxylation of an aryllithium en route to [¹⁴C]AZD469411

Directed deprotonation still plays a major role in the synthesis of labeled compounds. Elmore and coworkers formed the bisanion of thiophenol and reacted the anion with ¹⁴CO₂ to afford 2‐sulfhydrobenzoic acid (2) in poor yield; the product was then converted to thiazepine 3 (Scheme 3).13 Martinez and coworkers took advantage of the acidity of the protons on the methyl group of thioanisole to generate labeled 2‐(phenylthio)[2‐¹³C]acetic acid (4).12

Scheme 3.

A, Bisdeprotonation of thiophenol and subsequent carboxylation.¹² B, Deprotonation of thioanisole and carboxylation12

Modern methods for the preparation of Grignard reagents are now standard practice in radiochemistry labs. Latli and coworkers14 first formed the enolate of trifluormethylketone 5 using NaH and then the Grignard reagent using the procedure of Knochel15 (Scheme 4). The Grignard was then reacted with ¹⁴CO₂ and the resulting acid converted to glucocorticoid receptor antagonist 6 in 6 steps.

Scheme 4.

Carboxylation of an aryl Grignard reagent en route to glucocorticoid receptor antagonist 6 14

Similarly, Hickey and coworkers used the Knochel conditions to generate the 2‐chlorophenyl Grignard from the corresponding bromide (Scheme 5).16

Scheme 5.

Carboxylation of 1‐bromo‐2‐chlorobenzene via the Grignard reagent16

In an analogous fashion, Zhang generated 2‐bromo‐3‐cyanophenyl Grignard from the corresponding iodide (Scheme 6).17

Scheme 6.

Carboxylation of 2‐bromo‐3iodo‐benzonitrile via the Grignard reagent17

While the work of Hickey¹⁶ and Latli¹⁴ could likely have been accomplished using traditional methods, the arylnitrile in the substrate for Zhang¹⁷ might have precluded the formation of a organolithium or Grignard reagent by traditional means.

4. FUTURE OF CARBOXYLATIONS

While Gringard and organolithium reagents react directly with CO₂ to form carboxylic acids, their poor functional group compatibility ultimately limits their use. The incorporation of ¹⁴CO₂ via late stage functionalization would dramatically increase the applicability of this chemistry for the synthesis of labeled materials and avoid multistep conversions via nitrile formation‐hydrolysis pathways.18 We therefore present below a review of the current literature in the context of applicability to ¹⁴C carboxylation. The review is organized by product type (aromatic acid, benzylic acid, and aliphatic acid), with each area being subdivided by starting material.

4.1. Aromatic acids

In 2010, Knochel showed that organozinc reagents, generated from the corresponding bromo starting materials, reacted with CO₂ in the presence of MgCl₂ at 1 bar of pressure to give carboxylic acids in good yield (Scheme 7).19 Esters and nitriles were demonstrated to be compatible with the zinc reagent, but ketones and aldehydes reacted.

Scheme 7.

Zinc‐mediated carboxylation of aryl bromides19

In 2013, Daugulis demonstrated the use of a copper‐catalyzed carboxylation of aryl iodides, with a wide range of substrates (Scheme 8).20 The reaction proceeds at 1 bar of CO₂ with low catalyst loadings, but uses several equivalents of the pyrophoric reagent Et₂Zn. However, the reaction was shown to tolerate a wide range of functional groups, including bromo, fluoro, hydroxy, and ester moieties. Mechanistically, the reaction is believed to proceed via initial reduction of CuI to Cu(0) with Et₂Zn. Oxidative addition to the aryl iodide and subsequent reaction with CO₂ afford the copper(I) carboxylate. Finally, Et₂Zn reduction regenerates Cu(0) to complete the catalytic cycle.

Scheme 8.

Cu‐catalyzed carboxylation of aryl iodides20

Correa and Martin also developed a similar methodology using a phosphine containing palladium catalyst to carboxylate aryl bromides with Et₂Zn used to regenerate the catalyst.21 They investigated the effect of CO₂ pressure on the reaction and found that a pressure of 10 atm afforded the best yield and reduced the amount of proto‐debromination. While the reaction showed very good functional group compatibility, the dependence upon CO₂ pressure limits its application for radiochemical uses.

Tsuji and coworkers developed a nickel‐catalyzed carboxylation of aryl chlorides (Scheme 9).22 The method was tolerant of functional groups including esters, ketones, 3°‐amides, and boronic esters. However, alcohols, 2°‐amides, and ortho‐substituted arenes were not compatible with the reaction conditions. Importantly, the use of aryl chlorides gives access to a much larger supply of commercially available starting materials. Aryl bromides, aryl tosylates, and aryl triflates were also effective substrates for the reaction.

Scheme 9.

Ni‐catalyzed carboxylation of aryl chlorides22

The conversion of aryl tosylates to aryl carboxylic acids has been demonstrated by the group of Durandetti²³ using a catalyst system similar to that described by Tsuji²² (Scheme 10). The Durandetti method, however, effectively converts ortho‐substituted tosylates albeit at a slightly elevated temperature. This methodology demonstrates that phenols can be used as precursors for aryl carboxylates. Although not explicitly stated, it is likely that this procedure does not tolerate alcohols or amines. Not surprisingly, the procedures are also efficacious with aryl iodide and aryl bromide substrates.

Scheme 10.

Ni‐catalyzed carboxylation of aryl tosylates23

Cheng and coworkers showed that sodium arylsulfinates serve as efficient precursors of aryl carboxylic acids (Scheme 11).24 Under CuI catalysis, the aryl sulfinate is desulfinated with concomitant carboxylation or in a stepwise process via an arylcopper intermediate. The procedure requires elevated temperatures and prolonged reaction times in a sealed tube which will likely limit its application to radiochemistry. The reaction also shows some sensitivity to steric bulk as sodium 2,4,6‐trimethylbenzenesulfonate was efficiently converted to the corresponding carboxylic acid in 82% yield, but 2,4,6‐triisopropylbenzenesulfonate failed to afford the desired product.

Scheme 11.

Cu‐catalyzed carboxylation of aryl sulphinates24

The conversion of 2‐aryl‐5,5‐dimethyl‐1,3,2‐dioxaborinanes to aryl carboxylic acids has been shown to be effective using CuI catalysis and bisoxazoline ligand 7 (Scheme 12).25 The reaction was tolerant of functionality, but required 3 equivalents of CsF and 90°C. The yields of the reaction were higher when performed in a sealed tube rather than with a balloon of CO₂; the authors postulated that this was because of the sensitivity of the organometallic intermediate to water and oxygen.

Scheme 12.

Cu‐catalyzed carboxylation of arylboronic esters25

Hou and coworkers developed a procedure using a N‐heterocyclic carbene copper(I) complex ([(IPr)CuCl], 8) in refluxing THF to effect the same transformation (Scheme 13).26 This procedure was demonstrated to have broad functional group compatibility and was performed using a balloon of CO₂.

Scheme 13.

Cu‐catalyzed carboxylation of arylboronic esters26

Riss and coworkers extended this methodology to ¹¹C using a mixture of CuI, TMEDA, KF, and cryptofix‐222 in DMF (Scheme 14).27 Bromo, nitrile, nitro, and aldehyde functionalities were compatible with the reaction conditions and afforded products in radiochemical yields over 70%. However, hydroxy and amine containing substrates gave poor yields. They used this methodology to produce ¹¹C‐labeled oxytocin receptor radioligand 9 (Scheme 15). Compehensive reviews of ¹¹C carboxylations have recently been published by Gee and Vasdev.28, 29

Scheme 14.

Cu‐catalyzed ¹¹C carboxylation of arylboronic esters27

Scheme 15.

Synthesis of a ¹¹C‐labeled oxytocin receptor PET ligand 9 27

Hou and coworkers demonstrated that a combination of deprotonation ortho to a directing group to give an arylaluminum species followed transmetallation with [(IPr)CuCl] (8) and subsequent capture of the anion by CO₂ resulted in good to excellent yields of several carboxylic acids (Scheme 16).30 The reaction affords products with an excellent regioselectivity and modest functional group tolerance; nitriles, diisopropylamides, halides, an alkene, and a t‐butoxycarbonyl‐protected indole were unreactive under the conditions that were used. However, the reaction has a number of potential drawbacks for radiochemical synthesis. The reaction was performed under strict anhydrous conditions (glovebox) and uses triisobutylaluminum, which is very air and moisture sensitive. More significantly, the isobutyl groups of triisobutyl aluminum react with the CO₂ to generate isovaleric acid generating more by‐product than desired product (mol/mol). Obviously, this is a serious drawback for radiochemistry purposes, but perhaps the use of CO₂ as the limiting reagent might limit this by‐product, and the direct use of an arene is very attractive.

Scheme 16.

Directed deprotonation and Cu‐catalyzed carboxylation of arenes30

The Hou group has developed a method to carboxylate aromatic systems with relatively acidic protons (pKa = 25) (Scheme 17).31 The N‐heterocyclic carbene‐copper(I) complex [(IPr)CuCl] (8) deprotonates the arene to generate an arylcuprate which reacts with CO₂ to give the corresponding acid. While this works well for benzoxazoles (yields 50%‐86%) and tolerates halides, esters, nitros, and nitriles, it is much less effective for substrates with less acidic protons such as benzimidazoles, benzothiazoles, benzofurans, and 1,3,4‐oxadiazoles.

Scheme 17.

Deprotonation and Cu‐catalyzed carboxylation of benzoxazoles31

A more general method for carboxylating acidic heterocycles has been reported by Boogaerts and Nolan (Scheme 18).32 Under a pressure of 1.5 bar of CO₂, the N‐heterocyclic carbene gold(I) hydroxide complex [(IPr)AuOH] successfully carboxylates a range of heterocycles including oxazole, isoxazole, benzoxazole, thiazole, and N‐methylimidazole. The methodology was also extended to electron‐deficient arenes. The functional group capability for this reaction was not investigated.

Scheme 18.

Au‐catalyzed carboxylation of arenes and heteroarenes32

Cazin and coworkers also investigated the application of the N‐heterocyclic carbene‐copper(I) hydroxide complex [Cu(IPr)(OH)] for the same transformation, because of its ease of synthesis.33 Comparable results were achieved for benzoxazole, benzothiazole, oxazole, and electron‐deficient arenes. An inherent drawback for carboxylation of acidic substrates in labeled syntheses is the potential for the products to decarboxylate. However, the products may still prove useful as synthetic intermediates.

4.2. Benzylic acid

The method reported in Section 4.1.1 was also used by Knochel and coworkers to convert benzylic chlorides to phenylacetic acids (Scheme 19).19 The method was used to synthesize ibuprofen (10) from 1‐(1‐chloroethyl)‐4‐isobutyl‐benzene.

Scheme 19.

Zinc‐mediated carboxylation of benzyl chlorides19

Martin and coworkers also have developed a Ni‐catalyzed carboxylation of benzylic halides to afford phenyl acetic acids (Scheme 20).34 The method uses catalytic NiCl₂‐dimethoxyethane with zinc dust as the stoichiometric oxidant and tricyclopentylphosphine tetrafluoroborate to ligate the zinc. The reaction is run in DMF or DMA at room temperature. The addition of 2 equivalents of MgCl₂ improved the yield substantially, but the role of the metal is not clear. These conditions were not effective for secondary benzylic substrates, but substitution of tetrabutylammonium iodide for MgCl₂ and DMA for DMF gave modest yield of the target acids.

Scheme 20.

Ni‐catalyzed carboxylation of benzyl chlorides and bromides34

He and coworkers have demonstrated that benzyl chlorides can be carboxylated to afford phenylacetic acids using Pd catalysis (Scheme 21).35 The reaction is conducted with catalytic Pd(OAc)₂ ligated with 2‐dicyclohexylphosphino‐2′,6′‐dimethoxybiphenyl (SPhos) and a large excess of MgCl₂ and Mn in DMF at 0°C under 1 bar of CO₂. The reaction has a similar functional group compatibility to the Ni‐catalyzed reaction as substrates containing a chloride, an ester, a ketone, and a vinyl group were demonstrated to give good yields.

Scheme 21.

Pd‐catalyzed carboxylation of benzyl chlorides35

The Ni‐catalyzed method of Martin³⁴ and the Pd‐catalyzed reaction of He³⁵ give similar yields, have comparable functional group compatibility, and, operationally, appear to be of the same complexity. Therefore, for a primary benzylic chloride, either method is an appropriate choice. However, for a secondary or tertiary benzylic halide, the method of Martin has been demonstrated to work while the method of He will presumably lead to β‐elimination.

The Skrydstrup group (Scheme 22) have developed a method to carboxylate benzyl‐9‐borabicyclononane (benzyl‐9‐BBN) compounds using copper(I) catalysis (Scheme 22).36 Using 2 equivalents of CO₂ and [(IPr)CuF] formed in situ from CuI, 1,3‐bis‐(2,6‐diisopropylphenyl)imidazolinium, and 3 equivalents of CsF, they demonstrated that styrenes and stilbenes could be successfully carboxylated (via the benzyl‐9‐BBN adduct). The reaction was run at elevated temperatures and tolerated a wide range of substrates including phosphonates, thioethers, boronic acids, halides, and methylindoles. The regioselectivity is derived from the regiospecificity of the initial hydroboration.

Scheme 22.

Cu‐catalyzed carboxylation of benzyl‐9‐BBN compounds36

4.3. Aliphatic acid

Sawamura and coworkers developed a method to carboxylate alkyl‐9‐borabicyclononane (alkyl‐9‐BBN) compounds by using copper(I) catalysis (Scheme 23).37 The procedure consists of the addition of the alkyl‐9‐BBN compound in toluene to a solution of CuOAc, 1,10‐phenylanthroline, and KOtBu under 1 bar of CO₂. The reaction is heated at 100°C for 12 h to afford modest to good yields of the target alkyl acids. The main side product results from the protic deborylation. The reaction was demonstrated to tolerate silyl‐protected alcohols, esters, an acetal, a phthalimide, and a bromide. Secondary alkylboranes do not react under these conditions.

Scheme 23.

Cu‐catalyzed carboxylation of alkyl‐9‐BBN compounds37

A similar method has been developed by Hou which also uses copper(I) catalysis to convert alkyl‐9‐BBN compounds to acids (Scheme 24).38 In this case, [(IPr)CuCl] (8) was used as the catalyst with MeOLi as base in THF at 70°C. The functional group compatibility is the same as the Sawamura method³⁷ with the exception that an aryl iodide, a diaryl ketones, an aryl alkynes, and a thiophene were also demonstrated to be stable to the reaction conditions. In general, the yields were higher for this method; for example, aryl bromide 11 gave a 91% yield with the method of Hou versus 47% with that of Sawamura. However, 9‐BBN adduct of 1,1‐diphenylethylene gave a 54% yield using the method of Sawamura while the method of Hou failed to carboxylate the compound.

Scheme 24.

Cu‐catalyzed carboxylation of alkyl‐9‐BBN compounds38

The methodology of Skrydstrup reported in Section 4.2.2 has also been applied to affect the carboxylation of primary and secondary alkyl‐9‐BBN compounds (Scheme 25).36 The regioselectivity of the carboxylation is determined by the regiochemistry of the boronic acid.

Scheme 25.

Cu‐catalyzed carboxylation of primary and secondary alkyl‐9‐BBN compounds

Martin and coworkers have developed a method for the conversion of alkyl chlorides, bromides, and tosylates to alkylacids.39 While the procedure differs slightly for each substrate, the dimethoxyethane complex of NiCl₂ is used catalytically with a substituted phenanthroline ligand and Mn as the stoichiometric oxidant in DMF (chlorides and tosylates) or DMA (bromides) under 1 bar pressure of CO₂. The optimal conditions for the reaction with bromides (Scheme 26)39 occurred at room temperature; that of tosylates (Scheme 27)39 required heating to 50°C while chlorides (Scheme 28)40 required heating to 60°C and the addition of tetrabutylammonium bromide. The reaction of bromides was demonstrated to tolerate a wide range of functionality as was the reaction of chlorides. It is likely that the functional group tolerance of the reaction of tosylates will be similar to that of the chlorides, but that was not demonstrated. Three secondary and 1 tertiary chloride were also successfully carboxylated using the conditions similar to that developed for primary chlorides.

Scheme 26.

Ni‐catalyzed carboxylation of alkyl bromides39

Scheme 27.

Ni‐catalyzed carboxylation of alkyl tosylates39

Scheme 28.

Ni‐catalyzed carboxylation of alkyl chlorides40

5. CONCLUSION

The use of modern chemical methods to incorporate ¹⁴CO₂ has been very limited. However, the progress made over the past 10 years, detailed herein, demonstrates that excellent methods for late‐stage incorporation of labeled CO₂ exist. Method development will be required because all of these methods with the exception of that reported by Riss²⁷ use an excess amount of CO₂; however, it is likely that at least a few of these methods will be useful with stoichiometric quantities. All catalysts are commercially available thereby removing a frequent barrier to the use of the chemistry. A table summarizing the methods reported in this manuscript is presented herein (Table 1), indexed by reaction product and substrate. It details the functional group compatibility for each method.

Table 1.

Target acid with the methods reported and the demonstrated functional group compatibilities of the described methods

Target	Substrate	Method	Functional Group Tolerance										Incompatibility/Drawbacks	References
			Ester	Amide	Aldehyde	Ketone	Nitrile	Halide	Alkene	Alkyne	Nitro	Other
Aromatic
ArCO 2 H	ArI/ArBr	Zn activated by Mg	L	L				F				SiMe3, OSiR3, NMe2		Ref. 19
	ArI	CuI, ZnEt2	X			X		X				ArOH, indole NH		Ref. 20
	ArCl or ArBr	NiCl2, Mn	X	X		X		F				Boronic ester, OSiR3	Ortho substitution, alcohols, amines prohibited	Ref. 22
	ArOTs	NiCl2, Mn	X			X	X	F					Likely alcohols and amines	Refs. 22, 23
	ArOTf	NiCl2, Mn	X	X		X		F				Boronic ester, OSiR3	Ortho substitution, alcohols, and amines prohibited	Ref. 22
	ArSO 2 Na	CuI		X		X	X	Cl, Br					Requires sealed tube, 140°C	Ref. 24
	ArB(OR) 2	CuI	X			X	X	X	X	X	X		90°C, sealed tube (maybe), CsF	Ref. 25
		CuI	X	X	X	X	X	X	X	X	X	Epoxide, NR2, OSiR3, ArOH, ArNH2	KOtBu can lead to transesterification	Refs. 26, 27
	Directed C‐H insertion	Al, Cu		If sterically congested			X	X	X			SMe	Al(iBu)3, glove box	Ref. 30
HetCO 2 H	Benzoxazoles	Cu	X				X	X			X		KOtBu can lead to transesterification	Ref. 31
HetCO 2 H	Heterocycles with pH < 30	Au											Functional group compatibility not probed, but esters will likely hydrolyze because of use of KOH	Ref. 32
Benzylic
ArCH2 CO 2 H	ArCH2 Cl	Mg, ZnCl2	L	L				F				SiMe3, OSiR3, NMe2		Ref. 19
		NiCl2, MgCl2, Zn	X			X		F, cl	X	X		OSiR3		Ref. 34
		Pd(OAc)2, MgCl2, Mn	X			X		F, cl	X					Ref. 35
ArCHRCO 2 H	ArCHRCl	Mg, ZnCl2	L	L				F						Ref. 19
	ArCRHBr	NiCl2, MgCl2, Zn,	X			X		X						Ref. 34
ArCR2 CO 2 H	ArCR2 Br	NiCl2, MgCl2, Zn,											Only 1 example: Ph3Br	Ref. 34
ArCH(CO 2 H)CRR′	ArCH=CRR′ or ArCH(9‐BBN)CRR′	CuI				X		F, Cl				Thioether, phosphonate, indole	Silyl ethers, carbon‐carbon double and triple bonds.	Ref. 36
Alkyl
RR′CHCH2 CO 2 H	RR′CHCH2 (9‐BBN) or RR′C=CH2	Cu(I)	X	X				X		X		OSiR3		Refs. 37, 38
RCH(  CO 2 H)CRR′	RR′CHCHR″(9‐BBN) or RR′C=CR″H	CuI				X		F, Cl				Thioether, phosphonate, indole	Silyl ethers, carbon‐carbon double and triple bonds.	Ref. 36
RCH2CH2 CO 2 H	RCH2CH2 Br	NiCl2, Mn	X	Secondary	X	X	X	F, Cl				OTs, SnBu3, OH,		Ref. 39
	RCH2CH2 OTs	NiCl2, Mn	X										Functional group compatibility not probed, but should be similar to bromide	Ref. 39
	RCH2CH2 Cl	NiCl2, Mn	X	X	X	X	X	F	X			OSiR3, OH,		Ref. 40

X indicates that the compatibility was demonstrated. L indicates that while the carboxylation reaction was not probed with these functional groups, a very similar reaction is reported in which they are tolerated. For halides, X indicates that all F, Cl, Br, and I are stable to the conditions; otherwise, the specific halide that is tolerated is depicted.

Biographies

Ryan Bragg studied Chemistry at the University of Manchester (UK), staying on to study for his PhD in Organic Chemistry under the supervison of Prof. Jonathan Clayden. He then spent 1 year as a post‐doctoral fellow at the University of Geneva (Switzerland), in the group of Prof. E. Peter Kündig. After Medicinal Chemistry positions within British Biotech (UK) and Evotec OAI (UK), he then moved into the field of Isotope Chemistry at AstraZeneca (UK), initially as a Senior Scientist. He is currently an Associate Principle Scientist within the Isotope Chemistry Team in Cambridge (UK), with responsibility for delivering carbon‐14, tritium, and stable isotope‐labeled compounds. Ryan is an author of more than 25 publications and a patent owner.

Malvika Sardana graduated with a Bachelor's degree in Pharmaceutical Sciences and Master's degree in Medicinal Chemistry from the VU University Amsterdam (The Netherlands) in 2015. During her studies, she did a short internship with Griffin Discoveries (The Netherlands) supervised by Dr Mounir Andaloussi, major internship for her Master's degree at the VU University under the supervision of Dr Maikel Wijtmans, and an industrial internship at Boehringer Ingelheim (Austria) with Dr Simon Lucas as the supervisor. After graduating, she started working as a Senior Research Chemist in the department of Parallel Chemistry at Mercachem (The Netherlands). In 2017, she started as an Industrial PhD student at the Isotope Chemistry group of Dr Charles Elmore at AstraZeneca (Sweden). Her work is focused on the development of new methodologies of labeling with unlabeled, stable labeled, and radiolabeled carbon monoxide. Her PhD work is part of the ISOTOPICS project. The ISOTOPICS project has received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska‐Curie grant agreement no. 675071.

Markus Artelsmair studied Chemistry at Imperial College London, completing his BSc project under the supervision of Prof. Ed Tate. He then moved to pursue an MPhil at the University of Cambridge with Prof. Oren Scherman in the field of supramolecular catalysis. He is currently an industrial PhD student at AstraZeneca as part of the Marie Skłodowska‐Curie Innovative Training Network “PET3D,” working on PET radiotracers for the CCR2 receptor. Markus has received a number of awards for academic excellence throughout his studies and has published several scientific articles.

Charles (Chad) Elmore obtained a BS degree in Chemistry from Rose‐Hulman Institute of Technology where he performed research with Prof. Bruce Allison and a PhD from the University of Illinois‐Urbana, Champaign under the direction of Prof. Robert M. Coates. After graduation, he joined Merck Research Laboratories in Rahway, NJ as a Senior Research Fellow in the Labeled Compound Synthesis Group. After 7 years, he moved to AstraZeneca in Wilmington, DE in the Isotope Chemistry Group as a Scientist and later a group leader. He relocated with AstraZeneneca in 2011 to Mölndal, Sweden as the Director of Isotope Chemistry and is currently serving in that capacity. Chad currently has 3 graduate students, 2 of which are supported by the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska‐Curie Actions. Chad is the author of over 65 publications.

Bragg RA, Sardana M, Artelsmair M, Elmore CS. New trends and applications in carboxylation for isotope chemistry. J Label Compd Radiopharm. 2018;61:934–948. 10.1002/jlcr.3633