KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Bruchpilot | Developmental studies hybridoma bank |
DSHB Cat# nc82 RRID:AB_2314867 |
| Mouse monoclonal anti-cysteine string protein | Developmental studies hybridoma bank |
DSHB Cat# DCSP-1 (ab49) RRID:0041B_2307345 |
| Mouse monoclonal anti-GluRIIA | Corey Goodman | DSHB Cat# 8B4D2 (MH2B) RRID:AB_528269 |
| Rabbit polyclonal anti-Complexin | Huntwork and Littleton, 2007 |
PMID:17873870 |
| Rabbit polyclonal anti-Synaptotagmin 1 | Littleton et al., 1993 | PMID:8269841 |
| Rabbit polyclonal anti-GluRIIC | Aaron DiAntonio | PMID:15182665 |
| Rabbit polyclonal anti-Dlg | Corey Goodman | DSHB Cat# 4F3 anti-discs large RRID:AB_528203 |
| Mouse monoclonal anti-FLAG | Sigma Aldrich | F1804 SIGMA |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Philanthotoxin-433 | Santa Cruz Biotechnology |
sc-255421 |
| Experimental Models: Organisms/Strains | ||
| W | Bloomington Drosophila Stock Center |
#3605 |
| w; imdNP1182 | Kyoto Stock Center | #112555 |
| w, Tak12 | Bloomington Drosophila Stock Center |
#26272 |
| w, Tak1179 | Bloomington Drosophila Stock Center |
#26275 |
| w; IKKβCR | This work | N/A |
| w; RelE20 | Bloomington Drosophila Stock Center |
#9457 |
| w; GluRIIASP16 | Petersen et al., 1997 | PMID:9427247 |
| PGRP2 |
Choe et al., 2002 (ird72) |
PMID:11872802 |
| w, Df(1)BSC645 | Bloomington Drosophila Stock Center |
#25735 |
| w; Df(3R)BSC728 | Bloomington Drosophila Stock Center |
#26580 |
| w; Ok371-Gal4 | Mahr and Aberle, 2006 |
PMID:16378756 |
| BG57-Gal4 | Budnik et al., 1996 | PMID:8893021 |
| w; UAS-Tak1K46R-FLAG | This work | N/A |
| w−3605 | Yuh Nung Jan Lab | N/A |
| w; nos-Gal4VP14,UAS-cas9 | Kondo and Ueda, 2013 |
PMID:24002648 |
| Oligonucleotides | ||
| gRNA targeting IKKβ AAGTCAAGCTCAGCGAGCGT |
This paper | N/A |
| FWD primer for IKKβ target: CTTATAGCCTTTCGGCTGAGTTAG |
This paper | N/A |
| REV primer for IKKβ target: CCACCGTTACAGTATTCCAGC |
This paper | N/A |
| Tak1DN Duplex PCR primer a: caccATGGCCACAGCATCGCTGG |
This paper | N/A |
| Tak1DN Duplex PCR primer b: GAAGAACTCCcTGACGGCAA |
This paper | N/A |
| Tak1DN Duplex PCR primer c: TTGCCGTCAgGGAGTTCTTC |
This paper | N/A |
| Tak1DN Duplex PCR primer d: CGCATTGTGATGCGGTGGG |
This paper | N/A |
| Recombinant DNA | ||
| Plasmid: pCDF3-dU6:3gRNA | Simon Bullock | Addgene plamid #49410 |
| cDNA LD42274 | Drosophila Genomics Resource Center |
DGRC: 2729, Flybase ID: FBcl0166830 |
| pENTR/D-TOPO | Invitrogen | K240020 |
| pTWF (UAS-C-terminal FLAG) | Drosophila Genomics Resource Center |
DGRC barcode: 1116 |
| Software and Algorithms | ||
| Igor Pro 6.37 | Wavemetrics | N/A |
| MATLAB R2015a | Mathworks | N/A |