Mouse optical imaging for understanding resting-state functional connectivity in human fMRI

ABSTRACT

Resting-state functional connectivity (FC), which measures the temporal correlation of spontaneous hemodynamic activity between distant brain areas, is a widely accepted method in functional magnetic resonance imaging (fMRI) to assess the connectome of healthy and diseased human brains. A common assumption underlying FC is that it reflects the temporal structure of large-scale neuronal activity that is converted into large-scale hemodynamic activity. However, direct observation of such relationship has been difficult. In this commentary, we describe our recent progress regarding this topic. Recently, transgenic mice that express a genetically encoded calcium indicator (GCaMP) in neocortical neurons are enabling the optical recording of neuronal activity in large-scale with high spatiotemporal resolution. Using these mice, we devised a method to simultaneously monitor neuronal and hemodynamic activity and addressed some key issues related to the neuronal basis of FC. We propose that many important questions about human resting-state fMRI can be answered using GCaMP expressing transgenic mice as a model system.

Temporal correlation of spontaneous hemodynamic signals, commonly referred to as FC [1], is one of the most widely used fMRI methods to study functional network organization of the human brain in a non-invasive manner. The overall pattern of FC closely resembles anatomical connectivity [2] as well as effective connectivity assessed with electrical microstimulation [3,4]. Moreover, recent studies suggest that FC is sensitive enough to detect network-level functional changes due to behavioral training [5], wakefulness levels [6] and psychiatric diseases [7]. A key assumption underlying FC is that it reflects the large-scale spatiotemporal dynamics of spontaneous neuronal activity [8]. For appropriate interpretation of FC, however, we must verify its neuronal basis

Several groups have recently used transgenic mice expressing a genetically encoded calcium indicator (GCaMP) in neocortical neurons [9] to simultaneously observe neuronal calcium and optical intrinsic signals that reflect hemodynamic activity (Figure 1(a)). Some groups including ours monitored neuronal and hemodynamic activity in the entire dorsal neocortex of the transgenic mice using green (530 nm) and red (630 nm) channels, respectively [10–12]. At 630 nm, the optical intrinsic signal primarily reflects the deoxyhemoglobin signal [13]. Other groups have incorporated additional wavelengths to further disambiguate different hemodynamic components [14,15].

Using this method, we examined the relationship between fast spatiotemporal patterns of neuronal calcium activity and the spatial pattern of FC. We found a significant relationship between two seemingly different types of large-scale spontaneous neuronal activities – namely, global waves propagating across the neocortex and transient coactivations among cortical areas sharing high FC. Different sets of cortical areas, sharing high FC within each set, were coactivated at different timings of the propagating global waves, suggesting that spatial information of cortical network characterized by FC was embedded in the phase of the global waves (Figure 1(b)). Furthermore, we confirmed that such transient coactivations in calcium signal were converted into spatially similar coactivations in hemodynamic signal and were necessary to sustain the spatial structure of FC measured in hemodynamic signal. The present method also revealed that the conversion of the spatial pattern of neuronal activity to that of hemodynamic activity was not perfect. The accuracy of conversion between the spatial pattern of neuronal activity and that of hemodynamic activity was modulated as a function of the strength of the vascular signal and neuronal activity. In the presence of strong vascular signal or when neuronal activity was weak, the spatial pattern of neuronal activity was not faithfully converted into that of hemodynamic activity (Figure 1(c)). Together, these results explain how global waves of spontaneous neuronal activity propagating across large-scale cortical network contribute to FC measured in hemodynamic signal in the resting state.

Figure 1.

Simultaneous imaging of calcium and hemodynamic signals to investigate neuronal basis of resting-state FC.(a) Experimental setup. Neuronal activity is monitored with GCaMP fluorescence. Hemodynamic activity is monitored simultaneously with GCaMP by means of optical intrinsic signal. (b) Spatial organization of FC is encoded in the phase of globally propagating spontaneous neuronal activity. Phase is calculated relative to a region-of-interest indicated by white cross. (c) Probability of conversion from spatial pattern of neuronal Ca²⁺ signal to that of hemodynamics depends the strength of neuronal activity (left) and non-neuronal physiological noise as measured by the signal in large blood vessels (right). See [10] for details. (d) An example data showing similar temporal fluctuations of dynamic FC (30sec sliding-window) calculated using neuronal activity and dynamic FC calculated using hemodynamic activity.

The proposed method also allows us to ask questions related to newly developed analyses methods for FC in human fMRI [16]. In contrast with the traditional analysis of “static” FC using many minutes of scans, the temporal fluctuation of FC across short time windows gives the dynamic aspect of FC that could provide information on the functional organizations of healthy and diseased brains that is inaccessible with static FC [17–19]. The presence of temporal fluctuations in FC has also influenced theoreticians to constrain realistic models of brain networks [20–22]. However, it is unclear whether the fluctuations of FC measured in hemodynamics reflect the dynamics of underlying neural activity. Using simultaneous imaging of neuronal calcium and hemodynamic signals in transgenic mice, we found that the dynamics of FC calculated using hemodynamic signals closely resembled those calculated using calcium signals, suggesting a neuronal origin of the temporal fluctuations of hemodynamic FC (Figure 1(d)). Moreover, temporal fluctuations of spatial patterns across different short time windows were similar between FC calculated using neuronal calcium signal and FC calculated using hemodynamic signal.

The temporal fluctuation of FC across short time-windows does not necessarily indicate non-stationary FC. Indeed, recent studies using resting-state fMRI in humans reported that the temporal fluctuation of FC cannot be distinguished from that in a model assuming stationary FC and statistical sampling error [23,24]. Applying the same analysis to the mouse data, we found that, in both neuronal calcium and hemodynamic signals, the temporal dynamics of FC were not fully explained by stationary FC [16]. The difference may be attributed to the superior signal-to-noise ratio of mouse data, for both calcium and optical intrinsic signals, compared to human fMRI. Alternatively, the use of anesthetized preparation could have caused non-stationary FC in the mouse data. A recent report using simultaneous measurement of electrophysiological and optical intrinsic signals in local population of mouse somatosensory neurons showed that “spontaneous” hemodynamic activity is driven by behavior (e.g., whisking) and correlate only weakly with neuronal activity [25]. Whether or not such “spontaneous” hemodynamic activity contains spatial patterns relevant to resting-state FC remains unclear. An alternative possibility is that such “spontaneous” hemodynamic activity is global and thus mostly removed by the global signal regression typically used in the resting-state FC analysis. Future experiments using large-scale imaging in awake mice are needed to clarify these points.

There are several future applications of the proposed method. For example, resting-state FC in mouse models of mental diseases can be used to examine the possibility of diagnosis based on resting-state FC [26]. Genetic tools in mice also allows monitoring of cell-type and cortical layer-dependent neuronal activity [27], which is difficult in other model species such as primates [28]. Furthermore, because GCaMP mice allow monitoring of neuronal activity even at very early stages of development [29,30], developmental changes of resting-state FC can be tracked at a high signal-to-noise ratio. In summary, combined calcium and optical intrinsic signal imaging using GCaMP transgenic mice is becoming a powerful platform to clarify key issues in human fMRI.

Funding Statement

This work was supported by the Japan Society for the Promotion of Sciences [17K14931]; Japan Society for the Promotion of Sciences [18H05116]; AMED [Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS)]; Japan Society for the Promotion of Sciences [World Premium Institute (WPI)].

Acknowledgments

This work was supported by grants from Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS)—AMED (to K.O.), Japan Society for the Promotion of Science (JSPS) KAKENHI(Grant number 17K14931 and 18H05116 to T. Matsui and 18H06084 to T.Murakami), World Premium Institute (WPI), JSPS (to K.O.), Strategic International Research Cooperative Program (SICP)—AMED (to K.O.), Asashi Glass Foundation (to T.Matsui).

Disclosure statement

No potential conflict of interest was reported by the authors.