Fig. 5.

The effects on tumor growth after PCAT6 downregulation in vivo.

(a, b and c) The tumor growth curves were measured 4 days after the injection after injection of A549 cells stably transfected with shRNA PCAT6 1#, shRNA PCAT6 2# or empty vector and tumor volume was calculated once every 3 or 4 days. (d) Tumor weight when the tumors were harvested. (e) qRT-PCR analysis of PCAT6 expression level in tumor tissues formed from shRNA PCAT6 1#, shRNA PCAT6 2# or empty vector transfected A549 cells. (e) Representative images of HE staining and Ki-67 immunohistochemistry of the tumor. Up, H & E staining, Scale bar represents 50 μm; Down, immunostaining, Scale bar represents 20 μm. The data represent the mean ± S.D. from three independent experiments. *P < .05, **P < .01.