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. 2018 Dec 4;145(23):dev170811. doi: 10.1242/dev.170811

Fig. 3.

Fig. 3.

Behaviour of DBS-S in compromised cells that cannot complete the apoptosis programme. (A) Wing cells mutant for Dronc that ectopically express an activated form of Hemipterous under the control of spalt-Gal4 (SalG4) (DBS-S in green, cleaved caspase-3 in red). (B) Magnifications of the images shown in A. Notice the presence of cells positively labelled with DBS-S (nuclear signal of the reporter in green; white arrows) and cleaved caspase-3 immunoreactivity (red). (C,D) Identification and labelling of undead cells in wing imaginal discs expressing simultaneously Hemipterous activated and P35 under the control of Hedgehog-Gal4 (C) or simultaneously expressing Hid and P35 under the control of Hedgehog-Gal4 (D). xy images, high magnifications and cross-sections of wing epithelium (z-sections) are shown. DBS-S is shown in green, cleaved caspase-3 staining in red and P35 immunostaining in blue. The anterior (left side) and posterior (right side) compartments are separated by the discontinuous white line. The discontinuous yellow line indicates the location of the z-section in the xy image. White arrows indicate delaminated cells positively labelled with DBS-S (green). Blue and red arrowheads indicate the apical and basal parts of the wing epithelium, respectively.