Skip to main content
PMC home page
Frontiers in Physiology logoLink to Frontiers in Physiology
. 2018 Dec 4;9:1758. doi: 10.3389/fphys.2018.01758

Xenopus Hybrids Provide Insight Into Cell and Organism Size Control

Romain Gibeaux 1,†,‡, Kelly Miller 1,‡, Rachael Acker 1, Taejoon Kwon 2, Rebecca Heald 1,*
PMCID: PMC6288844  PMID: 30564147

Abstract

Determining how size is controlled is a fundamental question in biology that is poorly understood at the organismal, cellular, and subcellular levels. The Xenopus species, X. laevis and X. tropicalis differ in size at all three of these levels. Despite these differences, fertilization of X. laevis eggs with X. tropicalis sperm gives rise to viable hybrid animals that are intermediate in size. We observed that although hybrid and X. laevis embryogenesis initiates from the same sized zygote and proceeds synchronously through development, hybrid animals were smaller by the tailbud stage, and a change in the ratio of nuclear size to cell size was observed shortly after zygotic genome activation (ZGA), suggesting that differential gene expression contributes to size differences. Transcriptome analysis at the onset of ZGA identified twelve transcription factors paternally expressed in hybrids. A screen of these X. tropicalis factors by expression in X. laevis embryos revealed that Hes7 and Ventx2 significantly reduced X. laevis body length size by the tailbud stage, although nuclear to cell size scaling relationships were not affected as in the hybrid. Together, these results suggest that transcriptional regulation contributes to biological size control in Xenopus.

Keywords: Xenopus laevis, Xenopus tropicalis, hybrid, biological scaling, zygotic genome activation

Introduction

Biological size control and scaling are important and fundamental features of living systems. However, the molecular mechanisms that control size at the organism, cell, and subcellular levels are poorly understood. The frog Xenopus has emerged as a powerful system to explore nuclear and spindle size differences that occur between related species with different-sized eggs (Levy and Heald, 2010; Loughlin et al., 2011; Kitaoka et al., 2018), as well as subcellular scaling during early development, when cleavage divisions cause a rapid reduction in cell size (Good et al., 2013; Wilbur and Heald, 2013). We therefore set out to investigate whether Xenopus frogs could also be used to study size control at the level of the cell and the whole organism.

Cell size correlates strongly and linearly with genome size in a myriad of different organisms (Mirsky, 1951; Gregory, 2001; Cavalier-Smith, 2005), and increases in genome copy number through polyploidy have been shown to increase cell size within tissues or cell types (Lee et al., 2009; Frawley and Orr-Weaver, 2015). However, the molecular link between genome size and cell size remains an open question. Although increases in ploidy may globally affect gene expression, work in unicellular organisms such as yeast suggests that the maintenance of scaling between genome size and cell size does not simply reflect gene dosage (Galitski et al., 1999; Neumann and Nurse, 2007; Marguerat et al., 2012). Furthermore, the correlation between genome size and cell size is independent of the proportion of the genome that codes for genes (Gregory, 2001; Cavalier-Smith, 2005; Taft et al., 2007). A number of factors involved in many different processes, such as growth, metabolism and protein synthesis, development, differentiation, and cell cycle regulation (Björklund et al., 2006) can influence cell size in a variety of organisms, from bacteria, to yeast, to Drosophila, to mammals (Marguerat and Bähler, 2012). Many of these genes are conserved and contribute to tissue and organ size in a variety of multicellular organisms, however, how they influence organism size, and how organism size feeds back to organ/tissue/cell size to attain homeostasis remains unclear.

Interestingly, in the related frog species Xenopus laevis and Xenopus tropicalis, the size of the genome, cells, and component subcellular structures scale with body size (Brown et al., 2007; Levy and Heald, 2010). Furthermore, the larger allotetraploid Xenopus laevis (6.2 × 109 base pairs, N = 36 chromosomes, average body length 10 cm) and smaller diploid Xenopus tropicalis (3.4 × 109 base pairs, N = 20 chromosomes, 4 cm in length) can hybridize. While fertilization of an X. tropicalis egg with a X. laevis sperm produces an inviable hybrid embryo that dies as a late blastula (Gibeaux et al., 2018), fertilization of an X. laevis egg with a X. tropicalis sperm (le × ts) produces a viable adult frog intermediate in genome size (N = 28 chromosomes) and body length between the two species (Narbonne et al., 2011). This viable hybrid thus provides a unique in vivo vertebrate model for investigating biological size control at the organismal, cellular, and subcellular levels.

In this study, we characterized size scaling in viable le × ts hybrids and used this system to establish a novel screening method for candidate genes involved in size control to identify factors that affect the body size of the frog, as well as the scaling of its component cells and subcellular structures.

Results

Reduced Size in Viable le × ts Hybrids

Whereas, cross-fertilization of X. tropicalis eggs with X. laevis sperm produces hybrid embryos that die during zygotic genome activation (ZGA), the reverse cross of X. laevis eggs and X. tropicalis sperm (le × ts) results in viable hybrid embryos that possess genetic features of both X. laevis and X. tropicalis parents (Bürki, 1985; Lindsay et al., 2003; Narbonne et al., 2011; Elurbe et al., 2017; Gibeaux et al., 2018). Hybrid embryos progressed through tadpole, froglet, and adult stages (Figures 1A,B), although with significant morbidity. Using standard husbandry conditions for X. laevis, only four adults were obtained from hundreds of embryos in two separate attempts to generate le × ts hybrid frogs. Early development in the le × ts hybrid proceeded normally according to Nieuwkoop and Faber staging (Nieuwkoop and Faber, 1994) until the end of neurulation, and at a similar rate compared to wild type X. laevis embryos (Figure 1C, Supplementary Movie S1). However, by the tailbud stage, body length was significantly decreased in le × ts hybrids (Figure 1D). Relative shortening of body length continued, although development remained similar to X. laevis, and both hybrid and control animals initiated metamorphosis with the same timing (Figure 1E). As soon as metamorphosis was complete, size scaling stopped and the body length of both the le × ts hybrid and X. laevis froglets increased at the same rate, retaining the difference in size (Figure 1F). Strikingly, in adult hybrid frogs, both cell and nuclear size of erythrocytes was reduced (Figure 1G). Since the hybrid genome (28 chromosomes) is smaller than the X. laevis genome (36 chromosomes), these observations are consistent with genome size-dependent scaling at the organism, cellular, and subcellular levels.

Figure 1.

Figure 1

Open in a new tab

Growth and development of Xenopus le × ts viable hybrids. (A) Schematic of developmental outcomes of Xenopus laevis and Xenopus tropicalis cross-fertilization. (B) Agarose gel electrophoresis showing PCR amplification of 2 genomic loci in X. laevis (X. l, on chromosomes 5L and S) and one locus in X. tropicalis (X. t, chromosome 5). H1-10 indicates 10 randomly chosen hybrid tadpoles tested, confirming the consistent presence of all 3 subgenomes in hybrids. (C) Developmental timing in X. laevis and le × ts hybrid embryos. Average is plotted for each time point. Error bars show standard deviation. (D) Body length of tailbud stage X. laevis and le × ts hybrids. Box plots show all individual body lengths. Thick line inside box = average length, upper and lower box boundaries = ±standard deviation (SD). P-value was determined by two-tailed heteroscedastic t-test. Representative images of tailbuds at identical scale are shown on the right. (E) Body length of tadpoles throughout metamorphosis for X. laevis and le × ts hybrids. Average is plotted for each time point. Error bars show standard deviation. (F) Body length of X. laevis and le × ts hybrid froglets. Average is plotted for each time point. Error bars show standard deviation. Representative images of froglets at identical scale are shown on the right. (G) Size of erythrocyte cells and nuclei in X. laevis and le × ts hybrid adult frogs. Box plots show all individual cell or nuclear areas. Thick line inside box = average area, upper and lower box boundaries = ± SD. P-values were determined by two-tailed heteroscedastic t-test. Representative images of erythrocytes at identical scale are shown on the right.

Nuclear to Cell Size Scaling in le × ts Hybrids Is More Similar to That of X. laevis Haploids, Despite a Larger Genome Size

We wondered whether the scaling observed in hybrid embryos was due to the decrease in genome size, or if the paternal X. tropicalis genome also influenced size scaling. To examine the effect of altering genome size alone, we utilized haploid X. laevis embryos produced by fertilizing wild type X. laevis eggs with irradiated X. laevis sperm (Figure 2A). While the sperm DNA is inactivated and does not contribute to the genome of the offspring, the sperm centrosome induces embryonic development yielding haploid embryos containing only the N = 18 maternal genome (Hamilton, 1957). Haploid embryos developed normally to the tailbud stage, and at a similar developmental rate to wild type X. laevis embryos (Figure 2B, Supplementary Movie S2). However, by the tailbud stage, body length was significantly reduced in X. laevis haploids (Figure 2C). Haploid embryos never reach metamorphosis and stop developing as stunted tadpoles (Hamilton, 1963).

Figure 2.

Figure 2

Open in a new tab

Nuclear to cell size relationships pre- and post-zygotic genome activation in le × ts hybrids compared to X. laevis diploids and haploids. (A) Schematic of generation of haploid X. laevis tadpoles via UV irradiation of sperm. (B) Developmental timing in X. laevis and haploid X. laevis embryos. Average is plotted for each time point. Error bars show standard deviation. (C) Body length of tailbud stage X. laevis and haploid X. laevis. Box plots show all individual body lengths. Thick line inside box = average length, upper and lower box boundaries = ± SD. P-value was determined by two-tailed heteroscedastic t-test. (D) Nuclear diameter vs. cell diameter in X. laevis, X. laevis haploid, and le × ts hybrid embryos. (E) Nuclear diameter vs. cell diameter in X. laevis, X. laevis haploid, and le × ts hybrid embryos at developmental stages 6 and 8. (F) Nuclear diameter vs. cell diameter in X. laevis, X. laevis haploid, and le × ts hybrid embryos at developmental stage 10. (G) Nuclear diameter vs. cell diameter in X. laevis, X. laevis haploid, and le × ts hybrid embryos at developmental stage 21. For (E–G), we ran an analysis of covariance (ANOCOVA test) to determine whether the nuclear to cell size scaling significantly depends on the embryo types. At stage 6, p = 0.132, at stage 8, p = 0.126, at stage 10, p = 2.558 × 10−6, and at stage 21, p = 1.110 × 10−7.

We then evaluated nuclear to cell size scaling relationships before and after ZGA, comparing X. laevis, le × ts hybrids and haploid X. laevis embryos (Figures 2D–G). Interestingly, no difference in the nuclear to cell size ratio was observed at early stages (6 and 8) among the 3 embryo types (Figure 2E). However, we found that, from stage 10, haploid embryos possessed reduced nuclear sizes at similar cell sizes compared to X. laevis (Figure 2F). Consistent with their intermediate genome size (36 > 28 > 18 chromosomes), the scaling curve of hybrids fell between that of X. laevis and haploids. Strikingly however, by stage 21, nuclear to cell size scaling in hybrids was more similar to that of haploids than to wild type X. laevis (Figure 2G). Therefore, we hypothesized that upon ZGA, gene expression of the X. tropicalis paternal genome, rather than bulk genome size alone, contributes to the reduced size of le × ts hybrids.

Transcriptome Analysis Identifies 12 X. tropicalis Transcription Factors Expressed in Hybrids

To identify paternal X. tropicalis genes that could contribute to size control in hybrid embryos at ZGA, we performed RNA sequencing and transcriptome analysis of embryos at stage 9. We detected many tropicalis-derived paternally expressed genes in hybrid embryos. Differential expression analysis revealed one maternally expressed X. laevis gene that was significantly less abundant in the le × ts hybrid (Figure 3A, Supplementary Table S1), and 41 paternally expressed X. tropicalis genes that were significantly more abundant in le × ts hybrid, compared to X. laevis embryos (Figure 3B, Supplementary Table S1). Gene ontology (GO) term analysis of differentially expressed paternal genes revealed significant overrepresentation of the molecular function “DNA binding” (GO:0003677; 4.38-fold enrichment, with a 2.90e-3 false discovery rate), and of the biological process “transcription, DNA-templated” (GO:0006351; 4.65-fold enrichment, with a 3.93e-04 false discovery rate). Therefore, we conclude that transcriptional regulators with DNA binding functions are significantly enriched in paternally expressed genes in hybrid embryos. To finalize our list of candidates, we used Xenbase (James-Zorn et al., 2012; Karimi et al., 2018) to validate the transcription factor function of the candidate genes. From this, we set out to screen the following 12 transcription factors, Ers10, Hes7, Mix1, Ventx2, Foxi4, Sox3, Tgif2, Klf17, Sia2, Id3, Not, and Oct 25 as potential paternal scaling factors.

Figure 3.

Figure 3

Open in a new tab

Transcriptome analysis of le × ts hybrid embryos at the onset of zygotic genome activation. (A) Differential expression analysis of X. laevis maternal genes in stage 9 le × ts hybrid vs. X. laevis embryos. (B) Differential expression analysis of X. tropicalis paternal genes in stage 9 le × ts hybrid vs. X. laevis embryos. For both figures, RNA-seq reads are mapped to a database of combined X. laevis and X. tropicalis transcriptomes and significantly differentially expressed genes (DE; fold-change >2 and false discovery rate < 0.05) are marked in orange (see section Materials and Methods for more information).

X. tropicalis Transcription Factors Hes7 and Ventx2 Reduce Body Length in Tailbud Stage X. laevis Embryos

To test whether identified candidate transcription factors were responsible for reducing the size of hybrid embryos, we mimicked overexpression of each transcription factor (as in the le × ts hybrid) by microinjecting mRNA encoding each X. tropicalis candidate gene into fertilized one-cell X. laevis embryos. Cell and nuclear size were assessed in embryos fixed for immunofluorescence around the time of ZGA (10 h post-fertilization, ~ stage 10) and several hours post ZGA (24 h post-fertilization, ~ stage 21). Head to tail body length was measured at late tailbud stage, 48 h post-fertilization (Figure 4A). Two candidate transcription factors, Hes7 and Ventx2, significantly reduced overall body length (Figures 4B,C). Interestingly, the body length of embryos injected with Hes7 or Ventx2 was not significantly different from the body length in the le × ts hybrid (p = 0.64 and p = 0.48, respectively; two-tailed heteroscedastic t-test). It is not clear whether this effect is due to the level of overexpression, or to sequences differences between X. laevis and X. tropicalis proteins. Sequence comparisons revealed that X. laevis homeologs from L and S chromosomes share approximately 90% similarity, while the X. tropicalis Ventx2 and Hes7 from X. tropicalis are ~85% similar to the X. laevis proteins (Supplementary Figures S1A,B). The reduction in body length was however not accompanied by a change in nuclear to cell size scaling relationships as significant as that observed in the le × ts hybrid (Supplementary Figures S2A,B). To test whether co-expression of both genes had an additive or synergistic effect, Hes7 and Ventx2 were co-injected. This caused embryo death (27.39 ± 9.25% lethality on average) with viable embryos more similar in size distribution to Ventx2 than to Hes7-injected embryos (Supplementary Figure S2C; p = 0.18 and p = 0.04, respectively, two-tailed heteroscedastic t-test). We also observed significant embryo death in Sia2-injected embryos (to 100% by 48 h post-fertilization), preventing measurement at tailbud stage. Embryo death may be due to higher levels of Sia2 expression in injected embryos compared to the hybrid. However, neither nuclear nor cell size at stage 10 or 21 was altered as observed in hybrid embryos, indicating that death was not due to size scaling defects (Supplementary Figure S2D). Altogether, while the screen did not reveal factors that significantly affected cell and nuclear size, overexpression of either X. tropicalis Hes7 and Ventx2 resulted in a decrease in embryo size that could potentially contribute to organism size scaling in le × ts hybrids.

Figure 4.

Figure 4

Open in a new tab

Organismal size in X. laevis embryos upon overexpression of candidate X. tropicalis transcription factors. (A) Workflow of candidate scaling factor screen. (B) Body length of tailbud stage injected X. laevis embryos. Thick line inside box = average length, upper and lower box boundaries = ±SD. Stars indicate the significance of the pooled results of 3 experiments with p < 0.05 (two-tailed heteroscedastic t-test). Red coloring indicates significance of each 3 individual technical replicates with p < 0.05 (two-tailed heteroscedastic t-test). Reduced number of measured embryos in Not is due to the fact that, overall, 67.5% of injected-embryos exogastrulated, indicating a developmental defect. (C) Representative images of injected X. laevis embryos 48 h post-fertilization. Hes7- (left) and Ventx2-injected (right) are shown (top) with corresponding controls (bottom). Images are at identical scale.

Discussion

Little is known about how organisms scale in size and how size scaling is coordinated at the organismal, cellular, and subcellular levels. Uniquely, between the frogs X. laevis and X. tropicalis, linear size scaling is observed at the level of the genome, subcellular structures, cell, and organism. The specific factors that influence this phenomenon are unknown.

Generating viable le × ts hybrids intermediate in genome size, cell size, and body size between X. laevis and X. tropicalis allowed us to examine whether size scaling in Xenopus results from differences in genome size alone, or whether gene expression plays a role. While genome size clearly correlates with cell and organism size in le × ts hybrids, other factors likely influence these parameters. Even though the genome size of the le × ts hybrid is closer to that of a wild type X. laevis embryo, the nuclear to cell size scaling curve tracked more closely with that of haploid X. laevis embryos. Moreover, changes in nuclear to cell size ratios in le × ts hybrids began at zygotic genome activation, rather than in the early cleaving embryo, which lacks transcription and growth phases. It is therefore likely that size scaling in hybrids is at least in part a consequence of X. tropicalis paternal gene expression rather than from reduced genome size alone.

Two transcription factors from our screen, Ventx2 and Hes7, caused a significant decrease in body length, but did not cause a change in nuclear to cell size scaling as observed in the hybrid. Reduced head-to-tail body length may be caused by different mechanisms. Ventx transcription factors have been observed to maintain pluriopotency and inhibit cell differentiation during Xenopus embryogenesis (Scerbo et al., 2012). Therefore, overexpression may cause a developmental delay that interferes with tissue growth. Moreover, these transcription factors may affect only specific regions of the embryo. For example, in Hes7 injected embryos, tail length is shortened, whereas head and body length remain similar to X. laevis controls. Hes7 is essential for regulating somite segmentation in vertebrates via oscillatory expression in presomitic mesoderm. Dampening Hes7 oscillations either by deleting or overexpressing Hes7 causes somite fusion (Bessho et al., 2003; Takashima et al., 2011) which shortens tail length in mice (Hirata et al., 2004). Interestingly, mutation of Hes7 is similarly implicated in shortening tail length in cats (Xu et al., 2016) and miniaturization of dogs (Willet et al., 2015). It is also involved in human diseases such as spondylocostal dysostosis, which causes abnormal fusion of the bones of the ribs and spine, leading to a type of dwarfism characterized by a short trunk with normal-length arms and legs (Sparrow et al., 2013).

What then precisely regulates cell and subcellular scaling in le × ts hybrids, and how can gene expression influence these parameters? We propose a model whereby cell size in Xenopus is largely dictated by genome size, but can be “fine-tuned” by differential gene expression. Such differential gene expression can also influence organism size, which may be uncoupled from cell size. Our study illustrates an example of both a unique model system and a screening approach to study biological size control and scaling. Future experiments will take advantage of the improving Xenopus genome assemblies to identify and screen other candidate genes, particularly those involved in other biological processes such as growth factor signaling and cell proliferation.

Materials and Methods

Xenopus Frogs

All animal handling and procedures were performed according to the Animal Use Protocol approved by the UC Berkeley Animal Care and Use Committee. Mature X. laevis and X. tropicalis frogs were obtained from NASCO (Fort Atkinson, WI).

Generation of Viable Xenopus le × ts Hybrid Embryos

X. laevis females were primed with 100 IU of pregnant mare serum gonadotropin (PMSG, National Hormone and Peptide Program, Torrance, CA) at least 48 h before use and boosted with 500 IU of HCG (Human Chorionic Gonadotropin CG10, Sigma) 14–16 h before experiments. X. tropicalis males were primed with 250 IU of HGC 24 h before dissection. To obtain testes, X. tropicalis males were euthanized by anesthesia through immersion in double-distilled (dd)H2O containing 0.15% MS222 (tricaine) neutralized with 5 mM sodium bicarbonate before dissection. Testes were collected in Leibovitz L-15 media (Gibco – Thermo Fisher Scientific, Waltham, MA) supplemented with 10% Fetal Bovine Serum (FBS; Gibco), and stored at room temperature until fertilization. To prepare the sperm solution, one testis was added to 1 mL of ddH2O in a 1.5 mL microcentrifuge tube, and homogenized using scissors and a pestle. X. laevis females were squeezed gently to deposit eggs onto petri dishes coated with 1.5% agarose in 1/10X MMR. Any liquid in the petri dishes was removed and the eggs were fertilized with 1 mL of sperm solution per dish. Fertilized embryos were swirled in the solution to form a monolayer on the bottom of the petri dish and incubated for 10 min with the dish slanted to ensure submersion of eggs. Dishes were then flooded with 1/10X MMR, swirled and incubated for 10 min. To remove egg jelly coats, the 1/10X MMR was completely exchanged for freshly prepared Dejellying Solution (2% L-cysteine in ddH2O-NaOH, pH 7.8). After dejellying, eggs were washed extensively (>4X) with 1/10X MMR before incubation at 23°C. At Nieuwkoop and Faber stage 2–3, fertilized embryos were sorted and placed in fresh 1/10X MMR in new petri dishes coated with 1.5% agarose in 1/10X MMR.

Confirmation of Presence of Both X. laevis and X. tropicalis Genomes in le × ts Hybrids

Genomic DNA was extracted from le × ts hybrid embryos by incubating overnight in lysis buffer (50 mM Tris-HCl, 5 mM EDTA, 100 mM NaCl, 0.5% SDS) containing 250 μg/mL Proteinase K (Roche, Basel, Switzerland). DNA was isolated using Phenol-Chloroform extraction and ethanol precipitation. The genomic DNA was used as a PCR template for a single pair of primers that amplify a specific locus that differs ~100 bp in size between all 3 (sub)genomes. In X. tropicalis, the locus is on chromosome 5 and PCR product size is 510 bp. In X. laevis, one locus is on chromosome 5L for which the PCR product size is 408 bp and another one is on chromosome 5S for which the PCR product size is 305 bp. The sequences of the primer pair are fwd GTACTCTTCCCCAGCTTGCTG and rev GCCTGTATGGCTCCTAGGTTTTC.

Generation of Wild Type X. laevis Embryos for Microinjection

Ovulations, euthanasias, dissections, and fertilizations were carried out as described above for le × ts hybrids above, with the following modifications: X. laevis males were primed by injecting 500 IU of HCG 24 h before dissection. Dissected testes were collected in 1X Modified Ringer (MR) (100 mM NaCl, 1.8 mM KCl, 1 mM MgCl2, 5 mM HEPES-NaOH pH 7.6 in ddH2O), and stored at room temp for short periods, or at 4°C for up to 5 days. To make sperm solution, 1/3–1/2 of a testis was added to 1 mL of ddH2O in a 1.5 mL microcentrifuge tube.

Generation of Haploid X. laevis Embryos

Euthanasia of males and dissection/collection of testes proceeded as described for X. laevis males above. 1/3–1/2 of a testis was added to 1.1 mL of ddH2O in a 1.5 mL microcentrifuge tube and homogenized with scissors and a pestle. The tube was briefly centrifuged using a benchtop microcentrifuge for several seconds to pellet large pieces of tissue. One mL of supernatant was removed, avoiding pieces of tissue, and transferred to a non-coated glass petri dish. The open dish was placed into a UV-Crosslinker (Stratalinker, Stratagene) and the sperm solution irradiated twice using 30,000 microjoules. The solution was swirled between the two irradiations. The irradiated sperm solution was then retrieved and used for fertilization by depositing at least 0.5 mL solution on top of freshly squeezed X. laevis eggs in a petri dish coated with 1.5% agarose in 1/10x MMR. Fertilization, dejelly, and embryo storage then proceeded as described for le × ts hybrid embryos above.

Embryo Video Imaging

Imaging dishes were prepared using a homemade PDMS mold designed to print a pattern of 1 mm large wells in agarose that allowed us to image 4 embryos simultaneously within the 3 × 4 mm camera field of view for each type of embryo. Embryos were imaged from stage 2. X. laevis and le × ts hybrid or haploid videos were taken simultaneously using two AmScope MD200 USB cameras, (AmScope, Irvine, CA) each mounted on an AmScope SE305R stereoscope. Time lapse movies were acquired at a frequency of 1 frame every 10 s for 20 h and saved as Motion JPEG using a MATLAB (The MathWorks, Inc., Natick, MA) script. Movie post-processing (cropping, concatenation, resizing, addition of scale bar) was done using MATLAB and Fiji (Schindelin et al., 2012). All MATLAB scripts written for this study are available upon request. Two of the scripts used here were obtained through the MATLAB Central File Exchange: “videoMultiCrop” and “concatVideo2D” by Nikolay S.

Imaging and Measurement of Tailbud, Tadpole, and Frog Body Size

Tailbud stage embryos were placed in an ice-cold agarose-coated imaging chamber and imaged at 12 × magnification using a Wild Heerbrugg M7A StereoZoom microscope coupled to a Leica MC170HD camera and Leica LAS X software. Tadpoles were imaged by placing in a petri dish filled with a limited amount of water to prevent depth-biased measurements. Images were taken with an iphone camera, including a ruler in the field of view. Tadpole measurements were stopped when the tail began to recede at the end of metamorphosis. Froglets were placed in a transparent-bottom container placed on a ruler and fill with a minimal amount of water, and imaged with an iphone camera. Images were analyzed and length measured head to tail for tadpoles, or head to cloaca for froglets. Length measurements were done using the line tool in Fiji.

Erythrocyte Preparation and Measurements

A small drop of blood was collected from the frog foot with a sterile needle, and the drop was smeared on a slide. The smear was then fixed with methanol and stained with Giemsa stain (Sigma GS). Cell were imaged in brightfield using micromanager software (Edelstein et al., 2014) with an Olympus BX51 microscope equipped with an ORCA-II camera (Hamamatsu Photonics, Hamamatsu city, Japan).

RNA Isolation and Sequencing

To isolate RNA, embryos at stage 9 were homogenized mechanically in TRIzol® (Thermo Fisher Scientific, Waltham, MA) using up to a 30-gauge needle and processed according to manufacturer instructions. After resuspension in nuclease-free H2O, RNAs were cleaned using a RNeasy kit (Qiagen Inc.) according to manufacturer instructions. Libraries were prepared using manufacturer's non-standard specific RNA-seq library protocol with poly-A capturing mRNA enrichment method (Illumina, CA, USA). The paired-end 2 × 100 bp reads were generated by the Genome Sequencing and Analysis Facility (GSAF) at the University of Texas at Austin using Illumina HiSeq 2000. Transcriptome data generated in this study are available from NCBI Gene Expression Omnibus (Series record GSE118382).

Gene Expression Analysis

We mapped RNA-seq reads to the database of combined X. laevis and X. tropicalis transcriptome (available at http://genome.taejoonlab.org/pub/xenopus/annotation/; WorldCup_201407 version), using Bowtie1 (version 1.0). To prevent misalignment to other species, we applied stringent criteria, allowing no mismatches (–v 0), and ignoring a read mapped more than one target (–m 1). We estimated relative transcript abundance with “transcripts per million reads (TPM)” calculated by RSEM (version 1.2.19), and differential expression analysis was conducted using edgeR (version 3.36.1), with greater than 2-fold changes and false discovery rate (FDR) less than 0.05 cutoff to determine the significance.

Gene Ontology Analysis

We conducted Gene Ontology analysis with Panther DB (version 13.1). For statistical analysis for overrepresented terms, we used Fischer's exact test and FDR adjustment, and applied FDR < 0.05 as a significance cutoff. To validate our list of candidates, we searched Xenbase (http://www.xenbase.org/) using the gene name as the query.

Cloning and mRNA Synthesis of Candidate Transcription Factors

Total RNA was isolated from X. tropicalis embryos as described above in “RNA isolation and sequencing,” and cDNA was synthesized from RNA using the SuperScript III First Strand Synthesis system (Invitrogen- Thermo Fisher Scientific, Waltham, MA) according to manufacturer instructions. Transcription factor sequences were then PCR-amplified from the cDNA using the following primer sequences (all are written 5′-3′) concatenated with ~30 bp plasmid-homologous sequences: Esr10, fwd ATGGCTCCTTACAGCGCTAC, rev TTCTCTGGAGACCCTGGAAC; Sox3, fwd ATGTATAGCATGTTGGACAC, rev CTGTACCGCTCACTCACATA; Foxi4.2, fwd ATGAACCCAGTCCAGCAACC, rev CTTTGTACCAGGGAAGGTAC; Hes7.1, fwd ATGAAGGGAGCGAGTGAAGT, rev AGACCTGGAGACCTTGGGTA; Mix1, fwd ATGGACTCATTCAGCCAACA, rev TCTGTGTGCTCCTCCACCTT; Tgif2, fwd ATGATGAATTCGACTTTTGA, rev TCACGACAAGCACCCCCAAT; Ventx2.1, fwd ATGAACACAAGGACTACTAC, rev TTGGGCAGCCTCTGGCCTAC; Klf17, fwd ATGAGTGTGGCTTTCTCAAC, rev CATGTGTCTCTTCATGTGCAG; Not, fwd ATGTTACACAGCCCTGTCTTTC, rev CAGTTCAACATCCACATCATC; Oct25 fwd ATGTACAGCCAACAGCCCTTC, rev ACCAATATGGCCGCCCATGG; Sia2 fwd ATGACTTGTGACTCTGAGCTTG, rev GCCCCACATATCCGGATATTG; Id3 fwd ATGAAAGCCATCAGCCCAGTG, rev GTGGCAGACACTGGCGTCCC. These amplified sequences were then subcloned using Gibson assembly (New England Biolabs, Ipswich, MA) into a PCS2 expression vector obtained at the 2013 Advanced Imaging in Xenopus Workshop from the Wallingford lab (UT Austin, USA). mRNAs were synthetized from these expression constructs using mMessage mMachine SP6 Transcription Kit (Ambion – Thermo Fisher Scientific, Waltham, MA) following the manufacturer protocol. The mRNAs were then purified using Phenol-Chloroform extraction, resuspended in ddH2O, aliquoted and stored at −80°C.

Microinjection of Candidate Transcription Factors Into Xenopus Embryos

Stage 1 (one-cell) embryos about 30 min post-fertilization were transferred into a mesh-bottom dish containing 1/9X MMR 3% Ficoll for microinjection. Injections were done using a Picospritzer III microinjection system (Parker, Hollis, NH) equipped with a MM-3 micromanipulator (Narishige, Amityville, NY). To mimic overexpression of each transcription factor, each embryo was injected with 750 picograms of mRNA, a dose we determined was large enough to see phenotypes, but was not associated with embryo toxicity. Injected embryos were transferred to a new dish coated with 1.5% agarose in 1/10x MMR, and incubated at 23°C in 1/9X MMR 3% Ficoll for at least 6 h. The embryos were then transferred to fresh 1/10x MMR in a new agarose-coated dish, and incubated at 23°C with buffer changes into fresh 1/10x MMR several times daily until ready for fixation or imaging.

Embryo Whole Mount Immunofluorescence

Embryos at the desired developmental stage were fixed for 1 h using MAD fixative (2 parts methanol [Thermo Fisher Scientific, Waltham, MA], 2 parts acetone [Thermo Fisher Scientific, Waltham, MA]), 1 part DMSO [Sigma]). After fixation, embryos were dehydrated in methanol and stored at −20°C. Embryos were then processed as previously described (Lee et al., 2008) with modifications. Following gradual rehydration in 0.5 × SSC (1X SSC: 150 mM NaCl, 15 mM Na citrate, pH 7.0), embryos were bleached with 1–2% H2O2 (Thermo Fisher Scientific, Waltham, MA) in 0.5 × SSC containing 5% formamide (Sigma) for 2–3 h under light, then washed in PBT (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 0.1% Triton X-100 [Thermo Fisher Scientific, Waltham, MA]) and 2 mg/mL bovine serum albumin (BSA). Embryos were blocked in PBT supplemented with 10% goat serum (Gibco–Thermo Fisher Scientific, Waltham, MA) and 5% DMSO for 1–3 h and incubated overnight at 4°C in PBT supplemented with 10% goat serum and primary antibodies. The following antibodies were used to label tubulin and DNA, respectively: 1:500 mouse anti-beta tubulin (E7; Developmental Studies Hybridoma Bank, Iowa City, IA), and 1:500 rabbit anti-histone H3 (ab1791; Abcam, Cambridge, MA). Embryos were then washed 4 × 2 h in PBT and incubated overnight in PBT supplemented with 1:500 goat anti-mouse or goat anti-rabbit secondary antibodies coupled either to Alexa Fluor 488 or 568 (Invitrogen–Thermo Fisher Scientific, Waltham, MA). Embryos were then washed 4 × 2 h in PBT and gradually dehydrated in methanol. Embryos were cleared in Murray's clearing medium (2 parts of Benzyl Benzoate, 1 part of Benzyl Alcohol).

Confocal Imaging and Measurement of Embryos, Cells and Nuclei After Whole Mount Immunofluorescence

Embryos were placed in a chamber made using a flat nylon washer (Grainger, Lake Forest, IL) attached with nail polish (Sally Hansen, New York, NY) to a slide, filled with Murray's clearing medium, and covered by a coverslip (Beckman coulter, Brea, CA) for confocal microscopy. Confocal microscopy was performed on a Zeiss LSM 780 NLO AxioExaminer running the Zeiss Zen Software. Embryos were imaged using a Plan-Apochromat 20 × /1.0 water objective and laser power of 12%, on multiple 1024 × 1024 pixel plans spaced 0.68 μm apart in Z.

Nuclear area was measured in Fiji using the ellipse tool. From this, we calculated the diameter of a circle of the same area, a value that we could directly compare the cell size determined through the measurement of the cell diameter at the nucleus central plane. To test whether the nuclear to cell size scaling significantly depends on the embryo types or whether embryos were microinjected or not, we ran an analysis of covariance (ANOCOVA test) using the ‘aoctool’ in MATLAB with a “separate lines” model.

Protein Sequence Alignments

Multiple sequence alignments were performed using Clustal Omega (default parameters). Sequence identities and similarities were determined by pairwise alignments using EMBOSS Needle (default parameters).

Author Contributions

RH and RG designed the project. RG and RA characterized and measured nuclear and cell sizes in hybrids and haploids. TK performed the transcriptome analysis. KM and RG cloned the candidates and conducted the screen. KM measured nuclear and cell sizes in injected embryos. KM wrote the manuscript with inputs from RH and RG.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

We thank members of the Heald laboratory, present and past, for support and discussions, especially C. Cadart for critical reading of the manuscript. We thank E. M. Marcotte and colleagues at the Genome Sequencing and Analysis Facility (UT Austin) for RNA-seq analysis, and J. Chapman for assistance designing primers to loci on chromosome 5 for X. laevis and tropicalis. We also thank students K. Shih, M. Fitzsimmons, and S. Kawada for their dedicated assistance with experiments.

Footnotes

Funding. RG was initially supported by EMBO long-term fellowship ALTF 836-2013 and for most of this project by Human Frontier Science Program long-term fellowship LT 0004252014-L. KM was supported by the National Science Foundation Graduate Research Fellowship Program and National Institutes of Health training grant 2T32GM007232-36. RA was supported in part by a National Science Foundation REU Summer Fellowship in 2014. TK was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Science, ICT and Future Planning (NRF-2016R1C1B2009302), and the UNIST Research Fund (grant number 1.180063.01). RH was supported by NIH MIRA grant R35 GM118183 and the Flora Lamson Hewlett Chair. The confocal microscopy performed in this work was done at the UC Berkeley CRL Molecular Imaging Center, supported by National Science Foundation DBI-1041078.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fphys.2018.01758/full#supplementary-material

Supplementary Figure S1

Protein sequence alignments of Xenopus Ventx2 and Hes7. (A) Ventx2 protein sequences of X. tropicalis, X. laevis L, and S copies aligned using Clustal Omega. (B) Hes7 protein sequences of X. tropicalis, X. laevis L, and S copies aligned using Clustal Omega. For (A,B), identities and similarities were calculated using EMBOSS Needle.

Click here for additional data file. (537KB, TIF)
Supplementary Figure S2

Nuclear to cell size relationships post-zygotic genome activation in injected X. laevis embryos. (A) Nuclear diameter vs. cell diameter in Ventx2-injected X. laevis embryos at stage 10 and 21. Analysis of covariance at stage 10 gave a p-value of 0.865 and at stage 21 of 0.016. (B) Nuclear diameter vs. cell diameter in Hes7-injected X. laevis embryos at stage 10 and 21. Analysis of covariance at stage 10 gave a p-value of 0.820 and at stage 21 of 0.077. (C) Normalized body length of tailbud stage X. laevis embryos co-injected with Ventx2 and Hes7 (top). Percent fatality in Ventx2 and Hes7 co-injected embryos (bottom). Embryos were injected with 375 pg mRNA of each factor for a total injection of 750 pg. Thick line inside box = average length, upper, and lower box boundaries = ±SD. (D) Nuclear diameter vs. cell diameter in Sia2 injected X. laevis embryos at stages 10 and 21. Analysis of covariance at stage 10 gave a p-value of 0.171 and at stage 21 of 0.497.

Click here for additional data file. (530.8KB, tif)
Supplementary Table S1

Significantly differentially expressed transcripts in le × ts hybrids compared to X. laevis embryos.

Click here for additional data file. (12.3KB, XLSX)
Supplementary Movie S1

Characterization of le × ts hybrid embryo development. X. laevis vs. le × ts X. laevis eggs were fertilized with X. laevis (left) or X. tropicalis sperm (right). and simultaneously imaged in separate dishes. The video plays 20 h in 12 s (rate of 120 fps) and the scale bar corresponds to 200 μm.

Click here for additional data file. (20.7MB, AVI)
Supplementary Movie S2

Characterization of haploid X. laevis embryo development. X. laevis vs. haploid X. laevis. X. laevis eggs were fertilized with X. laevis (left) or irradiated X. laevis sperm (right) and simultaneously imaged in separate dishes. The video plays 20 h in 12 s (rate of 120 fps) and the scale bar corresponds to 200 μm.

Click here for additional data file. (26MB, AVI)

References

  1. Bessho Y., Hirata H., Masamizu Y., Kageyama R. (2003). Periodic repression by the bHLH factor Hes7 is an essential mechanism for the somite segmentation clock. Genes Dev. 17, 1451–1456. 10.1101/gad.1092303 [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Björklund M., Taipale M., Varjosalo M., Saharinen J., Lahdenperä J., Taipale J. (2006). Identification of pathways regulating cell size and cell-cycle progression by RNAi. Nature 439, 1009–1013. 10.1038/nature04469 [DOI] [PubMed] [Google Scholar]
  3. Brown K. S., Blower M. D., Maresca T. J., Grammer T. C., Harland R. M., Heald R. (2007). Xenopus tropicalis egg extracts provide insight into scaling of the mitotic spindle. J. Cell Biol. 176, 765–770. 10.1083/jcb.200610043 [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bürki E. (1985). The expression of creatine kinase isozymes in Xenopus tropicalis, Xenopus laevis laevis, and their viable hybrid. Biochem. Genet. 23, 73–88. [DOI] [PubMed] [Google Scholar]
  5. Cavalier-Smith T. (2005). Economy, speed and size matter: evolutionary forces driving nuclear genome miniaturization and expansion. Ann Bot. 147–175. 10.1093/aob/mci010 [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Edelstein A. D., Tsuchida M. a., Amodaj N., Pinkard H., Vale R. D., Stuurman N. (2014). Advanced methods of microscope control using μManager software. J. Biol. Methods 1:e10. 10.14440/jbm.2014.36 [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Elurbe D. M., Paranjpe S. S., Georgiou G., van Kruijsbergen I., Bogdanovic O., Gibeaux R., et al. (2017). Regulatory remodeling in the allo-tetraploid frog Xenopus laevis. Genome Biol. 18:198. 10.1186/s13059-017-1335-7 [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Frawley L. E., Orr-Weaver T. L. (2015). Polyploidy. Curr. Biol. 25, R353–R358. 10.1016/j.cub.2015.03.037 [DOI] [PubMed] [Google Scholar]
  9. Galitski T., Saldanha A. J., Styles C. A., Lander E. S., Fink G. R. (1999). Ploidy regulation of gene expression. Science 285, 251–254. 10.1126/science.285.5425.251 [DOI] [PubMed] [Google Scholar]
  10. Gibeaux R., Acker R., Kitaoka M., Georgiou G., Van Kruijsbergen I., Ford B., et al. (2018). Paternal chromosome loss and metabolic crisis contribute to hybrid inviability in Xenopus. Nature 553, 337–341. 10.1038/nature25188 [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Good M. C., Vahey M. D., Skandarajah A., Fletcher D. A., Heald R. (2013). Cytoplasmic volume modulates spindle size during embryogenesis. Science 342, 856–860. 10.1126/science.1243147 [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gregory T. R. (2001). Coincidence, coevolution, or causation? DNA content, cell size, and the C-value enigma. Biol. Rev. Camb. Philos. Soc. 76, 65–101. 10.1111/j.1469-185X.2000.tb00059.x [DOI] [PubMed] [Google Scholar]
  13. Hamilton L. (1957). Androgenic haploids of a toad, Xenopus laevis. Nature 179:159. 10.1038/179159a0 [DOI] [PubMed] [Google Scholar]
  14. Hamilton L. (1963). An experimental analysis of the development of the haploid syndrome in embryos of Xenopus laevis. J. Embryol. Exp. Morphol. 11, 267–278. [PubMed] [Google Scholar]
  15. Hirata H., Bessho Y., Kokubu H., Masamizu Y., Yamada S., Lewis J., et al. (2004). Instability of Hes7 protein is crucial for the somite segmentation clock. Nat. Genet. 36, 750–754. 10.1038/ng1372 [DOI] [PubMed] [Google Scholar]
  16. James-Zorn C., Ponferrada V. G., Jarabek C. J., Burns K. A., Segerdell E. J., Lee J., et al. (2012). Xenbase: expansion and updates of the Xenopus model organism database. Nucleic Acids Res. 41(Database issue), D865–D870. 10.1093/nar/gks1025 [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Karimi K., Fortriede J. D., Lotay V. S., Burns K. A., Wang D. Z., Fisher M. E., et al. (2018). Xenbase: a genomic, epigenomic and transcriptomic model organism database. Nucleic Acids Res. 46, D861–D868. 10.1093/nar/gkx936 [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kitaoka M., Heald R., Gibeaux R. (2018). Spindle assembly in egg extracts of the Marsabit clawed frog, Xenopus borealis. Cytoskeleton 75, 244–267. 10.1002/cm.21444 [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lee C., Kieserman E., Gray R. S., Park T. J., Wallingford J. (2008). Whole-mount fluorescence immunocytochemistry on Xenopus embryos. CSH Protoc. 2008:pdb.prot4957. 10.1101/pdb.prot4957 [DOI] [PubMed] [Google Scholar]
  20. Lee H. O., Davidson J. M., Duronio R. J. (2009). Endoreplication: polyploidy with purpose. Genes Dev. 23, 2461–2477. 10.1101/gad.1829209 [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Levy D. L., Heald R. (2010). Nuclear size is regulated by importin α and Ntf2 in Xenopus. Cell 143, 288–298. 10.1016/j.cell.2010.09.012 [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Lindsay L. L., Peavy T. R., Lejano R. S., Hedrick J. L. (2003). Cross-fertilization and structural comparison of egg extracellular matrix glycoproteins from Xenopus laevis and Xenopus tropicalis. Comp. Biochem. Physiol. Part A Mol. Integr. Physiol. 136, 343–352. 10.1016/S1095-6433(03)00169-7 [DOI] [PubMed] [Google Scholar]
  23. Loughlin R., Wilbur J. D., McNally F. J., Nedelec F. J., Heald R. (2011). Katanin contributes to interspecies spindle length scaling in xenopus. Cell 147, 1397–1407. 10.1016/j.cell.2011.11.014 [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Marguerat S., Bähler J. (2012). Coordinating genome expression with cell size. Trends Genet. 28, 560–565. 10.1016/j.tig.2012.07.003 [DOI] [PubMed] [Google Scholar]
  25. Marguerat S., Schmidt A., Codlin S., Chen W., Aebersold R., Bähler J. (2012). Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells. Cell 151, 671–683. 10.1016/j.cell.2012.09.019 [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Mirsky A. E. (1951). The desoxyribonucleic acid content of animal cells and its evolutionary significance. J. Gen. Physiol. 34, 451–462. 10.1085/jgp.34.4.451 [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Narbonne P., Simpson D. E., Gurdon J. B. (2011). Deficient induction response in a Xenopus nucleocytoplasmic hybrid. PLoS Biol. 9:e1001197. 10.1371/journal.pbio.1001197 [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Neumann F. R., Nurse P. (2007). Nuclear size control in fission yeast. J. Cell Biol. 179, 593–600. 10.1083/jcb.200708054 [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Nieuwkoop P. D., Faber J. (1994). Normal Table of Xenopus laevis (Daudin). New York, NY: Garland Publishing Inc. [Google Scholar]
  30. Scerbo P., Girardot F., Vivien C., Markov G. V., Luxardi G., Demeneix B., et al. (2012). Ventx factors function as Nanog-like guardians of developmental potential in Xenopus. PLoS ONE 7:e36855. 10.1371/journal.pone.0036855 [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Schindelin J., Arganda-Carreras I., Frise E., Kaynig V., Longair M., Pietzsch T., et al. (2012). Fiji: an open-source platform for biological-image analysis. Nat. Methods 9, 676–682. 10.1038/nmeth.2019 [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Sparrow D. B., Faqeih E. A., Sallout B., Alswaid A., Ababneh F., Al-Sayed M., et al. (2013). Mutation of HES7 in a large extended family with spondylocostal dysostosis and dextrocardia with situs inversus. Am. J. Med. Genet. Part A 161, 2244–2249. 10.1002/ajmg.a.36073 [DOI] [PubMed] [Google Scholar]
  33. Taft R. J., Pheasant M., Mattick J. S. (2007). The relationship between non-protein-coding DNA and eukaryotic complexity. Bioessays 29, 288–299. 10.1002/bies.20544 [DOI] [PubMed] [Google Scholar]
  34. Takashima Y., Ohtsuka T., Gonzalez A., Miyachi H., Kageyama R. (2011). Intronic delay is essential for oscillatory expression in the segmentation clock. Proc. Natl. Acad. Sci. U.S.A. 108, 3300–3305. 10.1073/pnas.1014418108 [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Wilbur J. D., Heald R. (2013). Mitotic spindle scaling during Xenopus development by kif2a and importin α. Elife 2:e00290. 10.7554/eLife.00290 [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Willet C. E., Makara M., Reppas G., Tsoukalas G., Malik R., Haase B., et al. (2015). Canine disorder mirrors human disease: exonic deletion in HES7 causes autosomal recessive spondylocostal dysostosis in miniature schnauzer dogs. PLoS ONE 10:e0117055. 10.1371/journal.pone.0117055 [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Xu X., Sun X., Hu X. S., Zhuang Y., Liu Y. C., Meng H., et al. (2016). Whole genome sequencing identifies a missense mutation in HES7 associated with short tails in Asian Domestic cats. Sci. Rep. 6:31583. 10.1038/srep31583 [DOI] [PMC free article] [PubMed] [Google Scholar]

Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Supplementary Figure S1

Protein sequence alignments of Xenopus Ventx2 and Hes7. (A) Ventx2 protein sequences of X. tropicalis, X. laevis L, and S copies aligned using Clustal Omega. (B) Hes7 protein sequences of X. tropicalis, X. laevis L, and S copies aligned using Clustal Omega. For (A,B), identities and similarities were calculated using EMBOSS Needle.

Click here for additional data file. (537KB, TIF)
Supplementary Figure S2

Nuclear to cell size relationships post-zygotic genome activation in injected X. laevis embryos. (A) Nuclear diameter vs. cell diameter in Ventx2-injected X. laevis embryos at stage 10 and 21. Analysis of covariance at stage 10 gave a p-value of 0.865 and at stage 21 of 0.016. (B) Nuclear diameter vs. cell diameter in Hes7-injected X. laevis embryos at stage 10 and 21. Analysis of covariance at stage 10 gave a p-value of 0.820 and at stage 21 of 0.077. (C) Normalized body length of tailbud stage X. laevis embryos co-injected with Ventx2 and Hes7 (top). Percent fatality in Ventx2 and Hes7 co-injected embryos (bottom). Embryos were injected with 375 pg mRNA of each factor for a total injection of 750 pg. Thick line inside box = average length, upper, and lower box boundaries = ±SD. (D) Nuclear diameter vs. cell diameter in Sia2 injected X. laevis embryos at stages 10 and 21. Analysis of covariance at stage 10 gave a p-value of 0.171 and at stage 21 of 0.497.

Click here for additional data file. (530.8KB, tif)
Supplementary Table S1

Significantly differentially expressed transcripts in le × ts hybrids compared to X. laevis embryos.

Click here for additional data file. (12.3KB, XLSX)
Supplementary Movie S1

Characterization of le × ts hybrid embryo development. X. laevis vs. le × ts X. laevis eggs were fertilized with X. laevis (left) or X. tropicalis sperm (right). and simultaneously imaged in separate dishes. The video plays 20 h in 12 s (rate of 120 fps) and the scale bar corresponds to 200 μm.

Click here for additional data file. (20.7MB, AVI)
Supplementary Movie S2

Characterization of haploid X. laevis embryo development. X. laevis vs. haploid X. laevis. X. laevis eggs were fertilized with X. laevis (left) or irradiated X. laevis sperm (right) and simultaneously imaged in separate dishes. The video plays 20 h in 12 s (rate of 120 fps) and the scale bar corresponds to 200 μm.

Click here for additional data file. (26MB, AVI)

Articles from Frontiers in Physiology are provided here courtesy of Frontiers Media SA

RESOURCES