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. Author manuscript; available in PMC: 2019 Nov 17.
Published in final edited form as: Cytokine. 2018 Nov 17;111:551–562. doi: 10.1016/j.cyto.2018.09.016

Figure 1. IL-2 induced phosphorylation of STATs 1 – 6 in the NKL cell line.

Figure 1.

Western blot analysis was performed on NKL cells that were cytokine starved overnight then treated for 30 min with or without IL-2 (100 U/mL). Total protein loaded per sample was 40 μg, with β-actin used as a loading control. Blots were probed for phosphorylated tyrosine and total protein forms: (A) STAT1, (B) STAT2, (C), STAT3, (D) STAT4, (E), STAT5, (F) STAT6. These are representative blots; the experiment was performed one other time with similar results.