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. Author manuscript; available in PMC: 2019 Nov 17.
Published in final edited form as: Cytokine. 2018 Nov 17;111:551–562. doi: 10.1016/j.cyto.2018.09.016

Figure 8. Intracellular IL-10 levels in NKL cells in response to calcitriol or EB1089 treatment.

Figure 8.

The total protein samples from the three independent experiments in Figures 2 – 5 were probed for intracellular IL-10 via western blot. Total protein loaded per sample was 40 μg with β-actin used as a loading control. Representative western blots of IL-10 (Figure 9A, B) are shown. Data from three independent experiments were quantified to determine relative expression levels and quantification is shown to the right of the representative blots. The fold change in the graphs represents IL-10 over corresponding β-actin. Repeated measures ANOVA was used to determine significance compared to 0 nM vehicle control (* p<0.05, ** p<0.01, *** p <0.001). For graphical representation, the vehicle control was set to 1.